RESUMO
The potentiation of infection is a recognized adverse effect of steroids. This study examined the in vitro effect of glucocorticoids on sheep alveolar macrophage phagocytic function, an essential means of cellular defence in the lungs, by evaluating the phagocytosis of zymosan particles after 30 min of contact between alveolar macrophages and steroids. Hydrocortisone, dexamethasone phosphate and methylprednisolone significantly reduced the phagocytic capability of sheep alveolar macrophages, but the effects of dexamethasone isonicotinate, prednisolone and flumethasone were less marked.
Assuntos
Glucocorticoides/farmacologia , Macrófagos Alveolares/efeitos dos fármacos , Fagocitose/efeitos dos fármacos , Animais , Líquido da Lavagem Broncoalveolar/citologia , Sobrevivência Celular , Células Cultivadas , Dexametasona/farmacologia , Isonicotinato de Dexametasona/farmacologia , Flumetasona/farmacologia , Hidrocortisona/farmacologia , Macrófagos Alveolares/citologia , Macrófagos Alveolares/fisiologia , Metilprednisolona/farmacologia , Prednisolona/farmacologia , Ovinos , ZimosanRESUMO
Broncho-alveolar lavages of sheep lungs were performed for alveolar macrophage identification and purification. The total number of harvested cells was 1.5 x 10(8) cells per 600 ml fluid lavage, with a mean of 2.48 x 10(5) cells/ml. Cell viability was 97.14%. Alveolar macrophages represented 78% of cells harvested. Separation was carried out by adherence to plastic flasks followed by Percoll centrifugation. The best results (purity of macrophage population and cell viability) was obtained with Percoll solution and NaCl 1.5 mol.l-1 with a density of 1.033 and 1.050. Identification was carried out by the non specific esterase staining method. In conclusion, broncho-alveolar lavage of isolated sheep lung is a simple and reliable method of respiratory tract cell collection.
