RESUMO
Leishmania infantum and Leishmania tropica are the species responsible for visceral leishmaniasis and cutaneous leishmaniasis respectively. In Greece, both diseases are endemic. The dog is considered the main reservoir of L. infantum, whereas the role of other animals for both L. infantun and L. tropica is unknown. Spleens from wild Rattus norvegicus, live trapped in Greece, were examined for the presence of Leishmania parasites by PCR. Out of 16 samples examined, only one was found positive for L. infantum with scant amount of parasitic DNA present. This is the first documented case of detection of L. infantum in R. norvegicus in Greece. The results of this preliminary study indicate that R. norvegicus is unlikely to be a reservoir for Leishmania parasites in Greece.
Assuntos
Leishmania infantum/genética , Leishmania tropica/genética , Leishmaniose Cutânea/veterinária , Leishmaniose Visceral/veterinária , Doenças dos Roedores/parasitologia , Animais , DNA de Protozoário/análise , DNA de Protozoário/genética , Reservatórios de Doenças , Grécia , Leishmania infantum/isolamento & purificação , Leishmania tropica/isolamento & purificação , Leishmaniose Cutânea/sangue , Leishmaniose Cutânea/parasitologia , Leishmaniose Visceral/sangue , Leishmaniose Visceral/parasitologia , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterinária , Ratos , Doenças dos Roedores/sangue , Doenças dos Roedores/epidemiologia , RoedoresRESUMO
Allele frequencies for 16 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA, and SE33) were calculated for a sample of 300 unrelated individuals from Greece. No deviations from Hardy-Weinberg equilibrium were observed for all loci. The combined power of discrimination (PD) and the combined power of exclusion (PE) for the 16 tested STR loci were 0.999999999 and 0.999999816, respectively. Population comparisons were carried out and low genetic distances were found between our data and those previously published for other neighbouring European populations.
Assuntos
Repetições de Microssatélites/genética , População Branca/genética , DNA/sangue , DNA/genética , DNA/isolamento & purificação , Europa (Continente) , Frequência do Gene/genética , Genética Populacional , Grécia , Humanos , Técnicas de Amplificação de Ácido Nucleico , Reação em Cadeia da Polimerase/métodosRESUMO
We report the absence of beta-catenin mutations in 63 sporadic colorectal carcinomas (SCRCs) with demonstrated decreased beta-catenin and E-cadherin mRNA expression and E-cadherin protein expression in a subset of carcinomas examined, suggesting that beta-catenin mutations are an extremely rare phenomenon in SCRCs and are not responsible for the transcriptional impairment of the beta-catenin/E-cadherin adhesion complex observed in these tumours.
