Galanin decreases spontaneous resting contractions and potentiates acetyl choline-induced contractions of goldfish gut.

Galanin (GAL) is a 29 amino acid peptide, first identified from the porcine intestine and widely distributed within the brain and peripheral tissues. Among GAL biological functions, its role as a potent appetite-stimulating peptide is probably the most studied. With galanin's established role in the modulation of food intake in fish, this study aims to evaluate the effects of GAL on the intestinal motility of the goldfish, Carassius auratus, using an organ bath system. Our results found that application of GAL to the organ bath causes a significant concentration-dependent decrease in the amplitude of spontaneous contractions of goldfish gut. Preincubations of intestinal strips with acetylcholine (ACh) and GAL showed that GAL increases the force of ACh-induced contractions of the goldfish gut. These results provide the first evidence for a role of GAL in gut motility in goldfish. This also suggests a crosstalk between the effects of GAL and ACh in such functions, thus pointing to a putative joint role between the two molecules. These findings offer novel information that strengthens the role of the galaninergic system in fish feeding.

Brain and intestinal expression of galanin-like peptide (GALP), galanin receptor R1 and galanin receptor R2, and GALP regulation of food intake in goldfish (Carassius auratus).

Galanin-like peptide (GALP) is a 60 amino acid neuropeptide originally discovered from porcine hypothalamus, and is involved in the regulation of food intake in mammals. Since its discovery, GALP and its receptors (GALR1 and GALR2) have been characterized in mammals, but no publications are available on GALP in fish and other non-mammals. The present study aimed to characterize brain and intestinal GALP and its receptors using immunohistochemistry in a teleost, the goldfish (Carassius auratus), and to study its effects on feeding behavior. Immunostaining of brain sections shows the presence of GALP- and GALR1- and GALR2-like immunoreactive cells in different encephalic areas, including the telencephalon, some hypothalamic nuclei, the optic tectum, the torus longitudinalis and the cerebellum. Signal for GALP was also observed in the fasciculus retroflexus. In the gut, GALP-and GALR1 and GALR2 immunoreactive cells were detected in the mucosa. Results from the feeding study demonstrate that intracerebroventricular administration of GALP (1ng/g bodyweight) increases goldfish food intake at 1h post-injection. These observations form the first report on the presence of GALP in the fish brain and gut, and also on its modulatory role on fish feeding behavior. GALP, as in mammals, appears to be a functional neuropeptide in goldfish.

Galanin systems in non-mammalian vertebrates with special focus on fishes.

Galanin is a well characterized multifunctional neuropeptide in mammals. Galanin has been identified from several fishes, amphibians, reptiles and birds. A large set of data is available on galanin-like protein distribution and peptide and/or mRNA sequences in non-mammalian vertebrates. Galanin receptor sequences from fishes and birds are known, but its distribution and mechanisms of actions are poorly understood. While some biological actions of galanin are known in non-mammals, the functional role of galanin in lower vertebrates is limited. For example, galanin has been shown to regulate feeding, pituitary hormone secretion and gut motility in fishes. Several aspects of galanin biology remain unknown, yet, there is enough evidence to implicate galanin as an important physiological modulator in lower vertebrates. Majority of the research articles on galanin in non-mammals arise from studies that used fishes. The objective of this chapter is to provide a summarized discussion of current knowledge on galanin peptide and gene sequences and organization, distribution of galanin gene and protein, and physiological functions of galanin in non-mammalian vertebrates with a special focus on fishes.