RESUMO
Quantitative anaerobic culture of urine samples obtained from 593 pregnant women by suprapubic bladder aspiration was performed to establish the involvement of anaerobic bacteria in asymptomatic urinary tract infections. The fluorescent antibody (FA) test was applied to the sediments of bladder aspirates to determine the site of infection. Anaerobic bacteriuria (greater than or equal to 10(4) microorganisms/ml of urine) was found in 34 patients, of whom five were FA-positive. These anaerobes were identified as Lactobacillus minutus, Veillonella parvula (two patients). Clostridium putrefaciens, and Peptostreptococcus anaerobius. Aerobic bacteriuria (greater than 10(4) microorganisms/ml of urine) was detected in 27 patients, of whom 13 were FA-positive. In 10 women with mixed aerobic/anaerobic bacteriuria, no FA-positive bacteria were found. The finding of FA-positive anaerobes may indicate that these organisms are involved in silent renal infection.
Assuntos
Anaerobiose , Anticorpos Antibacterianos , Bacteriúria/imunologia , Metabolismo , Adolescente , Adulto , Bacteroides/isolamento & purificação , Clostridium/imunologia , Clostridium/isolamento & purificação , Enterococcus faecalis/isolamento & purificação , Escherichia coli/isolamento & purificação , Eubacterium/isolamento & purificação , Ácidos Graxos/urina , Feminino , Imunofluorescência , Humanos , Lactobacillus/imunologia , Peptostreptococcus/imunologia , Peptostreptococcus/isolamento & purificação , Gravidez , Staphylococcus/isolamento & purificação , Veillonella/imunologiaRESUMO
The incidence of asymptomatic bacteriuria due to aerobic, facultative anaerobic and strictly anaerobic bacteria during pregnancy was determined in 185 patients admitted for complications of pregnancy, using suprapubic bladder aspiration. Anaerobic bacterial counts of 103 per ml urine or higher were detected in 6.4 per cent of the specimens. The incidence of anaerobic bacteriuria seems comparable to that of bacteriuria due to aerobic and facultative anaerobic micro organisms. In our group of patients the incidence of the latter was 5.4%. The presence of anaerobic bacteria was not confined to the occurrence of a particular species. Peptostreptococcus species, Veillonella species, Bacteroides species, Eubacterium species, Clostridium species and Bifidobacterium species were isolated from specimens in concentrations of more than 103 per ml. Consequences of asymptomatic bacteriuria during pregnancy are totally uncertain. Further investigation is necessary to obtain insight into the effect of bacteriuria due to anaerobes upon the outcome of pregnancy.
