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1.
Front Immunol ; 13: 1000006, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119038

RESUMO

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) led to a global health outbreak known as the COVID-19 pandemic which has been lasting since March 2020. Vaccine became accessible to people only at the beginning of 2021 which greatly helped reducing the mortality rate and severity of COVID-19 infection afterwards. The efficacy of vaccines was not fully known and studies documenting the immune responses following vaccination are continuing to emerge. Recent evidence indicate that natural infection prior vaccination may improve the antibody and cellular immune responses, while little is known about the factors influencing those processes. Here we investigated the antibody responses following BNT162b2 vaccination in relation to previous-infection status and age, and searched for possible biomarkers associated with the observed changes in immune responses. We found that the previous-infection status caused at least 8-times increase in the antibody titres, effect that was weaker in people over 60 years old and unaltered by the vitamin D serum levels. Furthermore, we identified adiponectin to positively associate with antibody responses and negatively correlate with pro-inflammatory molecules (MCP-1, factor D, CRP, PAI-1), especially in previously-infected individuals.


Assuntos
COVID-19 , Vacinas Virais , Adipocinas , Adiponectina , Anticorpos Antivirais , Formação de Anticorpos , Vacina BNT162 , COVID-19/prevenção & controle , Fator D do Complemento , Humanos , Pessoa de Meia-Idade , Pandemias , Inibidor 1 de Ativador de Plasminogênio , SARS-CoV-2 , Vacinação , Vitamina D , Vitaminas
2.
Rev Med Chir Soc Med Nat Iasi ; 116(4): 1171-6, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23700908

RESUMO

UNLABELLED: The aim of the present study was to investigate S. aureus isolates for the presence of methicillin-resistance and Panton-Valentine leukocidin (PVL) genes and to further characterize positive strains by means of antibiotic resistance patterns. MATERIAL AND METHODS: We used a triplex Real-Time PCR method for simultaneous detection of nuc, mecA and pvl genes in clinical isolates from 188 patients admitted to "Sf. Parascheva" Infectious Diseases Hospital lasi, during a 3 year period (2008-2010). RESULTS: The study revealed a relatively high rate of PVL-producing strains (23.93%), mainly community-associated (CA-MRSA) (51.11%). Most pvl-positive CA-MRSA isolates were resistant to erythromycin (91.3%), but none was resistant to clindamycin, fluoroquinolones, rifampicin, chloramphenicol or fusidic acid. CONCLUSIONS: Antibiotic susceptibility testing showed a high rate of multidug-resistance among strains classified as CA-MRSA (54.83%), but not among PVL-producers (4.44%). Although resistance to fusidic acid was previously proposed as a marker for PVL-producing CA-MRSA, our data suggest that we cannot rely on resistance to fusidic'acid to screen for PVL-producing CA-MRSA in our setting.


Assuntos
Proteínas de Bactérias/genética , Toxinas Bacterianas/genética , Exotoxinas/genética , Leucocidinas/genética , Staphylococcus aureus Resistente à Meticilina/genética , Nuclease do Micrococo/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Biomarcadores/sangue , Infecções Comunitárias Adquiridas/microbiologia , Humanos , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Staphylococcus aureus Resistente à Meticilina/patogenicidade , Testes de Sensibilidade Microbiana , Proteínas de Ligação às Penicilinas , Fenótipo , Romênia , Infecções Estafilocócicas/microbiologia
3.
Rev Med Chir Soc Med Nat Iasi ; 115(2): 531-5, 2011.
Artigo em Romano | MEDLINE | ID: mdl-21870752

RESUMO

UNLABELLED: Resistance to carbapenems by KPC (Klebsiella pneumoniae carbapenemase) production in Klebsiella pneumoniae clinical isolates was first described ten years ago in the U.S.A. and recently reported in other countries. This enzyme inactivates all beta-lactam antibiotics and is associated with fluoroquinolone and aminoglycoside resistance. MATERIAL AND METHODS: We investigated the carbapenem resistance in 498 Escherichia coli and Klebsiella pneumoniae strains isolated from urinary tract infections during 2009 in the lasi "Dr. C. I. Parhon" Clinical Hospital. Antimicrobial susceptibility testing was performed according to CLSI guidelines. To detect ESBL (extended spectrum beta-lactamase) and KPC production we used phenotypic tests and molecular biology methods (PCR). RESULTS: From all tested strains, only two K. pneumoniae strains showed modified susceptibility to carbapenems. The modified Hodge test was positive for the strain resistant to ertapenem, meropenem, imipenem (KA) and negative for the strain resistant only to ertapenem (K(B)). Both KA and K(B) isolates were negative for blaKPC and blaTEM genes, but harbored blaSHV and bla(CTX-M) genes, respectively. CONCLUSIONS: We report the first carbapenem resistant K. pneumoniae strains from Northeast Romania. The resistance is not mediated by KPC-carbapenemase; the possibility of dual mechanisms through cefalosporinases production and porins loss is suggested.


Assuntos
Proteínas de Bactérias/biossíntese , Carbapenêmicos/farmacologia , Escherichia coli/enzimologia , Klebsiella pneumoniae/enzimologia , Infecções Urinárias/tratamento farmacológico , Infecções Urinárias/microbiologia , beta-Lactamases/biossíntese , Antibacterianos/farmacologia , Carbapenêmicos/uso terapêutico , Ertapenem , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Humanos , Imipenem/farmacologia , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/isolamento & purificação , Meropeném , Testes de Sensibilidade Microbiana , Fenótipo , Reação em Cadeia da Polimerase , Porinas/genética , Tienamicinas/farmacologia , beta-Lactamas/farmacologia
4.
Rev Med Chir Soc Med Nat Iasi ; 115(4): 1208-13, 2011.
Artigo em Romano | MEDLINE | ID: mdl-22276471

RESUMO

UNLABELLED: The aim of our study was to evaluate the antimicrobial susceptibility profile and the presence of metallo-beta-lactamases (MBLs) among carbapenem-resistant Pseudomonas aeruginosa clinical isolates. MATERIAL AND METHODS: A total of 84 P. aeruginosa clinical isolates collected between January 2007- February 2011 from four university hospitals in Iasi (North-East region of Romania) were randomly selected. Antimicrobial susceptibility testing was performed according to CLSI 2010 (Clinical and Laboratory Standards Institute) guidelines. The isolates were tested for MBLs using EPI (EDTA-phenanthroline-imipenem) phenotypic test and polymerase chain reaction (PCR) for bla(VIM) and bla(IMP). RESULTS: Fifty-eight carbapenem resistant strains were identified, from which 24 (41,3%) were positive for VIM-type MBLs. No IMP - type MBL was detected. All MBL-producing isolates displayed a MDR (multidrug resistant) phenotype, two of them were XDR (extensively drug-resistant). Colistin remained the most effective antibiotic. CONCLUSIONS: The high proportion of MBL producing P. aeruginosa clinical isolates urges the need for a better use of antibiotics and for efficient infection control measures to prevent dissemination of MBL producers. This is the first report of VIM-like enzymes in P. aeruginosa isolates from the Iasi area.


Assuntos
Antibacterianos/farmacologia , Carbapenêmicos/farmacologia , Farmacorresistência Bacteriana/genética , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/genética , beta-Lactamases/genética , Antibacterianos/uso terapêutico , Carbapenêmicos/uso terapêutico , Humanos , Testes de Sensibilidade Microbiana , Infecções por Pseudomonas/tratamento farmacológico , Pseudomonas aeruginosa/isolamento & purificação
6.
Rev Med Chir Soc Med Nat Iasi ; 109(3): 638-41, 2005.
Artigo em Romano | MEDLINE | ID: mdl-16607764

RESUMO

A 42-year-old man was diagnosed with hepatosplenic T-cell lymphoma in November 2003. Remission was incompletely achieved despite 10 courses of chemotherapy. He developed fever (38.5-39 degrees C), chills, cough and morning expectoration after the 61 courses of chemotherapy. During this period, an ultrasound of the abdomen showed multiple hypodense lesions of the liver. Culture of the sputum yielded Scedosporium apiospermum. Fungal cultures from blood remained negative. The patient was treated with voriconazol. After one month from therapy, an ultrasound examination showed decreasing of hypodense lesions of the liver, and no further lesions appeared. Based on significant improvement of liver lesions with voriconazole therapy, we proposed the diagnostic "probable" disseminated infections due to S.apiospermum.


Assuntos
Hospedeiro Imunocomprometido , Linfoma Anaplásico de Células Grandes/complicações , Micetoma/microbiologia , Infecções Oportunistas/microbiologia , Scedosporium/isolamento & purificação , Adulto , Antifúngicos/uso terapêutico , Evolução Fatal , Humanos , Linfoma Anaplásico de Células Grandes/diagnóstico , Linfoma Anaplásico de Células Grandes/tratamento farmacológico , Masculino , Micetoma/complicações , Micetoma/diagnóstico , Micetoma/tratamento farmacológico , Infecções Oportunistas/complicações , Infecções Oportunistas/diagnóstico , Infecções Oportunistas/tratamento farmacológico , Pirimidinas/uso terapêutico , Triazóis/uso terapêutico , Voriconazol
7.
Rev Med Chir Soc Med Nat Iasi ; 109(2): 387-91, 2005.
Artigo em Romano | MEDLINE | ID: mdl-16607806

RESUMO

Modified Hodge test and a method using a disk with imipenem plus 1000 mg of EDTA were used to determine the presence of metallo-beta-lactamase producing gram-negative rods among 166 clinical isolates from hospitals in Iasi and Galati. Of 9 imipenem resistant strains found, only one Pseudomonas aeruginosa gave positive results with both tests and other two P. aeruginosa clinical isolates gave negative results with both tests. The rest of the strains (2 P. aeruginosa, 2 Acinetobacter baumanii, 1 Sphingomonas paucimobilis) did not give conclusive results. These screening methods are useful, simple and accessible to clinical laboratories. PCR is needed to confirm the presence of metallo-beta-lactamase gene in bacteria and to determine the type of the enzymes.


Assuntos
Proteínas de Bactérias/análise , Bactérias Gram-Negativas/enzimologia , Pseudomonas aeruginosa/enzimologia , beta-Lactamases/análise , Antibacterianos/farmacologia , Proteínas de Bactérias/biossíntese , Sangue/microbiologia , Carbapenêmicos/farmacologia , Líquido Cefalorraquidiano/microbiologia , Farmacorresistência Bacteriana , Bactérias Gram-Negativas/efeitos dos fármacos , Humanos , Programas de Rastreamento , Testes de Sensibilidade Microbiana , Estudos Multicêntricos como Assunto , Derrame Pleural/microbiologia , Reação em Cadeia da Polimerase , Pseudomonas aeruginosa/efeitos dos fármacos , Romênia , Supuração/microbiologia , Urina/microbiologia , beta-Lactamases/biossíntese
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