Injection of the diaphragmatic crus with methylene blue for coloration of the canine thoracic duct.

OBJECTIVE: To describe and compare onset and intensity of thoracic duct (TD) coloration after injection of methylene blue into the diaphragmatic crus and mesenteric lymph node. STUDY DESIGN: Experimental study. ANIMALS: Adult dogs (n = 18). METHODS: Methylene blue (≤0.5 mg/kg 1% solution) was injected into the left (n = 9) or right (n = 9) diaphragmatic crus via right 10th intercostal thoracotomy. TD coloration was graded over 10 minutes. A right paracostal laparotomy was then performed in all dogs, and an equal volume of methylene blue injected into a mesenteric lymph node (n = 18). TD color grading was repeated. Statistical analysis was performed on subject weight, volume of contrast agent injected between left and right crus, and number of successful outcomes between diaphragmatic crus injection and mesenteric lymph node injection. RESULTS: TD coloration occurred in 6 dogs with left crus injection and 4 dogs with right crus injection with obvious staining present in 2 and 3 dogs, respectively. Successful outcome was noted in all dogs with mesenteric lymph node injection. The number of successful outcomes was significantly greater after mesenteric lymph node injection compared with diaphragmatic crus injection (P < .001). CONCLUSIONS: Methylene blue injected into the diaphragmatic crura and mesenteric lymph node was successful in coloring the TD; however, mean thoracic duct color grade and number of successful outcomes were significantly higher after mesenteric injection.

Diaphragmatic support of a thoracic wall defect in a dog.

A large, caudal thoracic mass was removed along with ribs 11 and 12, resulting in an approximate 16 x 14-cm, caudal thoracic wall defect in a dog. The diaphragmatic musculature was mobilized and used to support the thoracic wall defect. To our knowledge, this method of thoracic wall repair has not been previously reported. This procedure allowed for airtight closure of the thoracic cavity, provided physical support, eliminated the need for muscle flaps or commercially available meshes, and provided a good cosmetic appearance without negatively affecting the dog's athletic performance.