Bone marrow trephine biopsy involvement by lymphoma: pattern of involvement and concordance with flow cytometry, in 10 years from a single institution.

PURPOSE: To evaluate the features of bone marrow (BM) biopsy involvement by lymphoma, pattern of infiltration, morphological analysis and flow cytometry were reviewed at all lymphoma patients over a period of 10 years. METHODS/PATIENTS: 413 cases were included in the study if BM biopsy slides were available. Only 356 patients had both BM trephine biopsy and flow cytometry. RESULTS: The most frequent subtype was diffuse large B cell (31.2%), followed by follicular lymphoma (18.9%). The predominant pattern was mixed (nodular-interstitial) (9.2%). Morphological marrow infiltration was found in 138 cases, and flow cytometry identified 117 cases with BM involvement. A concordance between the two methods was detected in 305 cases (85.7%). There was discordance in 51 cases (14.3%): morphology positive/FC negative in 33 cases and morphology negative/FC positive in 18. CONCLUSIONS: Flow cytometry is slightly more useful in detecting involvement when the BM is affected, but this finding is not conclusive.

Comparison between a conventional technique and two bone regeneration techniques in periradicular surgery.

AIM: The aim was to demonstrate the efficacy of two materials for bone regeneration during periradicular surgery and their effects on the healing of periapical tissues. METHODOLOGY: Twenty-eight patients (30 surgical sites) were selected and distributed into three groups:group A, conventional technique; group B, conventional technique plus nonbioabsorbable GoreTex R Augmentation membrane; and group C, conventional technique plus the same membrane placed over a synthetic bioactive resorbable graft of a hydroxylapatite (OsteoGen) product in the bony defect. Clinical and radiological evaluations were completed immediately prior to surgery, a week later and every 3 months after surgery up to 12 months. Two histological evaluations were performed (at the beginning and at 12 months). RESULTS: The results showed complete clinical and radiographic healing (eight cases) for group C, with histologic evidence of trabecular bone in all cases. For group B, six out of nine cases showed complete radiographic healing, incomplete in one case and uncertain in two cases, with histologic evidence of trabecular bone in five out of eight cases, scar tissue in one case and granuloma in two cases. For group A, there was complete radiographic healing in four out of nine cases, incomplete in four and unsuccessful in one case, with evidence of granuloma in four out of eight, scar tissue in two cases and trabecular bone in two cases. CONCLUSIONS: It was concluded that the conventional technique was less predictable in its healing response during the 12 months of this study. The use of bone regeneration materials, such as nonbioabsorbablemembranes and resorbable hydroxylapatite improved the predictability of clinical, radiographic and histological healing.