Acute exposure to perfluorooctane sulfonate exacerbates heat-induced oxidative stress in a tropical coral species.

Perfluorooctane sulfonate (PFOS) is among the most commonly per- and poly-fluoroalkyl substances (PFAS) found in environmental samples. Nevertheless, the effect of this legacy persistent organic contaminant has never been investigated on corals to date. Corals are the keystone organisms of coral reef ecosystems and sensitive to rising ocean temperatures, but it is not understood how the combination of elevated temperature and PFOS exposure will affect them. Therefore, the aims of the present study were (1) to evaluate the time-dependent bioconcentration and depuration of PFOS in the scleractinian coral Stylophora pistillata using a range of PFOS exposure concentrations, and (2) to assess the individual and combined effects of PFOS exposure and elevated seawater temperature on key physiological parameters of the corals. Our results show that the coral S. pistillata rapidly bioconcentrates PFOS from the seawater and eliminates it 14 days after ceasing the exposure. We also observed an antagonistic effect between elevated temperature and PFOS exposure. Indeed, a significantly reduced PFOS bioconcentration was observed at high temperature, likely due to a loss of symbionts and a higher removal of mucus compared to ambient temperature. Finally, concentrations of PFOS consistent with ranges observed in surface waters were non-lethal to corals, in the absence of other stressors. However, PFOS increased lipid peroxidation in coral tissue, which is an indicator of oxidative stress and enhanced the thermal stress-induced impairment of coral physiology. This study provides valuable insights into the combined effects of PFOS exposure and ocean warming for coral's physiology. PFOS is usually the most prevalent but not the only PFAS defected in reef waters, and thus it will be also important to monitor PFAS mixture concentrations in the oceans and to study their combined effects on aquatic wildlife.

Preferential grazing and repackaging of small polyethylene microplastic particles (≤ 5 µm) by the ciliate Sterkiella sp.

Microplastic (MP) particles are pollutants of global concern and are ubiquitously distributed in the ocean by physical and biological processes. It has been shown that zooplankton can ingest MP yet the interaction between ciliates and MP is still poorly understood. The discrimination and preferential uptake of MP rather than algal prey by ciliates was assessed in this study. The ciliate Sterkiella sp. was fed a diet that consisted of only Isochrysis galbana or a mixture of the same algae and similarly sized polyethylene beads in a 1:3 ratio. Significant, preferential MP grazing was observed in the Plastic-Algae treatment, which is the first reported evidence of proto-zooplankton preferentially ingesting MP over algal food. The mixed treatment contained fecal pellets with embedded MP. Preferential uptake of MP suggests that Sterkiella sp. is capable of ingesting and then "repackaging" MP that would otherwise be too small for larger taxa. This process would thus offer a mechanism for the reintroduction of MP into different compartments of the marine food web. As a consequence, it is necessary to account for small-sized MP (<5 µm) particles, that may have additional and yet unknown, impacts on marine food webs.

Large-scale survey of lithium concentrations in marine organisms.

Trace metals such as Cu, Hg, and Zn have been widely investigated in marine ecotoxicological studies considering their bioaccumulation, transfer along trophic webs, and the risks they pose to ecosystems and human health. Comparatively, Li has received little attention, although this element is increasingly used in the high-tech, ceramics/glass, and medication industries. Here, we report Li concentrations in more than 400 samples, including whole organisms and different organs of bivalves, cephalopods, crustaceans, and fish. We investigated species from three contrasting biogeographic areas, i.e. temperate (Bay of Biscay, northeast Atlantic Ocean), tropical (New Caledonia, Pacific Ocean), and subpolar climates (Kerguelen Islands, southern Indian Ocean), among diverse trophic groups (filter-feeders to meso-predators) and habitats (benthic, demersal, and pelagic). Although Li is homogeneously distributed in the ocean (at 0.18 µg/mL), Li concentrations in soft tissues vary greatly, from 0.01 to 1.20 µg/g dry weight. Multiple correspondence analyses reveal two clusters of high and low Li concentrations. Li distributions in marine organisms appear to be mostly geographically independent, though our results highlight a temperature dependency in fish muscles. Li is consistently bio-reduced through the trophic webs, with filter-feeders showing the highest concentrations and predatory fish the lowest. Strong variations are observed among organs, consistent with the biochemical similarity between Na and Li during transport in the brain and in osmoregulatory organs. Fish gills and kidneys show relatively high Li concentrations (0.26 and 0.15 µg/g, respectively) and fish brains show a large range of Li contents (up to 0.34 µg/g), whereas fish liver and muscles are Li depleted (0.07 ± 0.03 and 0.06 ± 0.08 µg/g, respectively). Altogether, these results provide the first exhaustive baseline for future Li ecotoxicology studies in marine coastal environments.

210Po bioaccumulation and trophic transfer in marine food chains in the northern Arabian Gulf.

The tendency of 210Po to concentrate in body tissue poses a serious concern of radiological safety. This study compiles available information and presents recent 210Po data for the marine food web in the northern Gulf waters. Since 210Po is concentrated in marine biota, a large number of samples of various marine organisms covering several trophic levels, from microalgae to sharks, were analyzed. 210Po was found to be highly concentrated in several marine species with the highest 210Po concentrations found in yellowfin tuna, i.e. 37.3-44.9, 451-548, and 1511-1693 Bq kg-1 wwt in muscle, digestive system and liver, respectively. In most dissected fish samples, 210Po showed increasing concentrations in the following order: edible tissue, gills, digestive system, liver and fecal matter. Fish feces had 210Po concentrations several orders of magnitude higher than that in seawater, fish muscle, and the fishes' ingested food. The high 210Po concentration in fish fecal matter suggests that the bulk of 210Po content in fish is eventually excreted back into the environment as fecal pellets. In most fish high concentrations were noted in liver, with the highest 210Po concentration recorded in yellowfin tuna liver. Moreover, 210Po concentration in the soft tissue of tunicate and bryozoan samples were 872-1012 and 402-527 Bq kg-1 wwt, respectively, far higher than that in fish muscle (0.04-44.9 Bq kg-1 wwt). It was observed that the maximum 210Po concentration in edible fish tissue among the fish in trophic level 2 was an order of magnitude lower than those in trophic level 3 and two orders of magnitude lower compared to fish in trophic level 4. The highest concentrations in the muscle tissue were observed in the following order: tunicate > bryozoan > mollusc > crustacean > algae > fish. Among all the biota analyzed, the highest overall concentration of 210Po was noted in yellowfin tuna (Thunnus albacores) indicating a potential biomagnification of 210Po in this particular top predator species. In general, 210Po concentrations found in the commercially important fish from Kuwaiti waters were comparable to levels that have been reported for similar fish species from several other marine areas worldwide.

First description of the neuro-anatomy of a larval coral reef fish Amphiprion ocellaris.

The present study described the neuro-anatomy of a larval coral reef fish Amphiprion ocellaris and hypothesized that morphological changes during the transition from the oceanic environment to a reef environment (i.e. recruitment) have the potential to be driven by changes to environmental conditions and associated changes to cognitive requirements. Quantitative comparisons were made of the relative development of three specific brain areas (telencephalon, mesencephalon and cerebellum) between 6 days post-hatch (dph) larvae (oceanic phase) and 11 dph (at reef recruitment). The results showed that 6 dph larvae had at least two larger structures (telencephalon and mesencephalon) than 11 dph larvae, while the size of cerebellum remained identical. These results suggest that the structure and organization of the brain may reflect the cognitive demands at every stage of development. This study initiates analysis of the relationship between behavioural ecology and neuroscience in coral reef fishes.

Meeting the food and nutrition needs of the poor: the role of fish and the opportunities and challenges emerging from the rise of aquaculture.

People who are food and nutrition insecure largely reside in Asia and Sub-Saharan Africa and for many, fish represents a rich source of protein, micronutrients and essential fatty acids. The contribution of fish to household food and nutrition security depends upon availability, access and cultural and personal preferences. Access is largely determined by location, seasonality and price but at the individual level it also depends upon a person's physiological and health status and how fish is prepared, cooked and shared among household members. The sustained and rapid expansion of aquaculture over the past 30 years has resulted in >40% of all fish now consumed being derived from farming. While aquaculture produce increasingly features in the diets of many Asians, it is much less apparent among those living in Sub-Saharan Africa. Here, per capita fish consumption has grown little and despite the apparently strong markets and adequate biophysical conditions, aquaculture has yet to develop. The contribution of aquaculture to food and nutrition security is not only just an issue of where aquaculture occurs but also of what is being produced and how and whether the produce is as accessible as that from capture fisheries. The range of fish species produced by an increasingly globalized aquaculture industry differs from that derived from capture fisheries. Farmed fishes are also different in terms of their nutrient content, a result of the species being grown and of rearing methods. Farmed fish price affects access by poor consumers while the size at which fish is harvested influences both access and use. This paper explores these issues with particular reference to Asia and Africa and the technical and policy innovations needed to ensure that fish farming is able to fulfil its potential to meet the global population's food and nutrition needs.

Biokinetics of Hg and Pb accumulation in the encapsulated egg of the common cuttlefish Sepia officinalis: radiotracer experiments.

Uptake and depuration kinetics of dissolved (203)Hg and (210)Pb were determined during the entire embryonic development of the eggs of the cuttlefish, Sepia officinalis (50d at 17 degrees C). (203)Hg and (210)Pb were accumulated continuously by the eggs all along the development time reaching load/concentration ratio (LCR) of 467+/-43 and 1301+/-126g, respectively. During the first month, most of the (203)Hg and (210)Pb remained associated with the eggshell indicating that the latter acted as an efficient shield against metal penetration. From this time onwards, (203)Hg accumulated in the embryo, indicating that it passed through the eggshell, whereas (210)Pb did not cross the chorion during the whole exposure time. It also demonstrated that translocation of Hg associated with the inner layers of the eggshell is a significant source of exposure for the embryo. This study highlighted that the maturing embryo could be subjected to the toxic effects of Hg in the coastal waters where the embryonic development is taking place.

Bioaccumulation and detoxification processes of Hg in the king scallop Pecten maximus: field and laboratory investigations.

Hg bioaccumulation was investigated in the king scallop Pecten maximus in the laboratory and in the field. In controlled conditions, scallops were exposed to (203)Hg through seawater, sediment and food in order to determine its uptake and depuration kinetics. In the field, Hg and metallothionein (MT) concentrations and the metal subcellular distribution were determined in scallops from two sites of the Bay of Seine (France) differently subjected to the Seine river inputs. While Hg concentrations in the whole soft parts and kidneys (viz. the highest accumulator organ) did not differ between scallops from both sites (74-156 ng g(-1)dry wt), they did for the digestive gland and the gills. According to the experimental results, a higher exposure to dissolved Hg might occur in the site close to the estuary whereas Hg would be mainly incorporated via the dietary pathway in the site away from the estuary. Within the cells of wild scallops, Hg was mainly associated to the cytosolic fraction in the digestive gland and gills (60-100%). However, the lack of relationship between Hg and MT levels suggests that Hg detoxification in P. maximus involves other, non-MT, soluble compounds. In kidneys, insoluble compounds played an important role in Hg sequestration. No effect of scallop age was observed neither on Hg and MT concentrations nor on the subcellular distribution of the metal. Finally, according to FAO/WHO recommendations (maximum weekly Hg intake), our results clearly indicate that the low Hg contents in the edible part of the king scallops from the Bay of Seine prevent any risk for human consumers.

Accumulation of nine metals and one metalloid in the tropical scallop Comptopallium radula from coral reefs in New Caledonia.

Uptake of waterborne Cd, Co, Mn and Zn was determined in laboratory experiments using radiotracer techniques (109Cd, 57Co, 54Mn and 65Zn). Labelled Zn was mainly accumulated in the digestive gland (65%) and Co in kidneys (81%); Cd and Mn were similarly distributed in digestive gland and gills. In a complementary field study, Ag, As, Cd, Co, Cr, Cu, Fe, Mn, Ni, and Zn were analysed in scallops collected at two stations showing different contamination levels. Digestive gland and kidneys displayed the highest concentrations. Ag, As, Cd, and Fe differed in soft tissues from the two stations, suggesting that Comptopallium radula could be a valuable local biomonitor species for these elements. Low Mn and Zn concentrations found in kidneys suggest that their content in calcium-phosphate concretions differs from the other pectinids. Preliminary risk considerations suggest that As would be the only element potentially leading to exposure of concern for seafood consumers.

Nickel bioaccumulation in bivalves from the New Caledonia lagoon: seawater and food exposure.

The New Caledonian lagoon is submitted to intense heavy metal input from land-based Ni mining. Therefore, the use of sentinel species is strongly recommended in order to develop and implement coastal zone management programmes of the metal contamination. The tropical oysters Isognomon isognomon and Malleus regula and the clam Gafrarium tumidum were previously proposed as such possible sentinel organisms and were thus investigated in this context. The three species were exposed to Ni via seawater or food using radiotracer techniques. Results indicate that uptake and retention efficiencies of Ni are independent of the dissolved Ni concentrations in the surrounding seawater. Hence, for the three species, body concentrations of Ni taken up from the dissolved phase are directly proportional to the ambient dissolved concentrations. Biokinetic patterns indicated that the major part of Ni was rapidly lost from bivalves during the first days of depuration, whereas 7 to 47% of 63Ni were retained in tissues with a biological half-life not significantly different from infinity. Finally, feeding experiments showed that Ni ingested with food (phytoplankton) was assimilated more efficiently in clams (assimilation efficiency, AE = 61%) than in oysters (AE = 17%), and was strongly retained (T(b1/2) > or = 35 d) in the tissues of both bivalves. It is concluded that the investigated species examined are efficient bioaccumulators of Ni from both the surrounding seawater and the food, and that they would be useful bioindicators for monitoring the status of Ni contamination in tropical coastal waters.

Allometric relationships in the bioconcentration of heavy metals by the edible tropical clam Gafrarium tumidum.

Although metal contamination is a problem of major concern in the lagoon of New Caledonia due to intense mining activities conducted on land, very little is known on the metal ecotoxicology of local marine organisms. The clam Gafrarium tumidum was investigated to assess its usefulness as a bioindicator species of metal contamination in this lagoon. More particularly, allometric relationships between metal accumulation and clam size were determined for five common metals in New Caledonian lagoon waters (Cd, Cr, Co, Zn and Ag) using a highly sensitive radiotracer technique. Experimental results showed that allometric relationships were dependent on the element and on the body compartment considered. As a rule, allometric relationships of metal concentration factor were more pronounced in shell than in soft parts. Significant relationships with clam size for Cd, Cr, Co and Zn followed inverse power functions. In contrast, the degree of Ag bioaccumulation was positively correlated with size. In view of the literature on Ag in bivalves, the latter observation suggests the occurrence of a specific detoxification mechanism (sequestration) that would be more efficient in old individuals. Overall, the experimental results indicate that the use of G. tumidum as a bioindicator in monitoring programmes requires selecting individuals of a specific size range in order to obtain comparable information about ambient metal levels. Since the size effect is greatest among smaller individuals, it is recommended to select clams with a shell width greater than 35 mm.