1.
Mol Biol Rep
; 10(4): 227-30, 1985 Oct.
Artigo
em Inglês
| MEDLINE
| ID: mdl-4069108
RESUMO
Preincubation of synaptosomes in standard physiological medium stimulates 2-fold Ca2+ uptake as compared to non-preincubated synaptosomes. When the sodium concentration in the preincubation medium has been halved, Ca2+ uptake was reduced by approximately 50 percent. The addition of ouabain to the preincubation medium decreases depolarization-stimulated Ca2+ uptake by about 40 percent. A steady-state level of Ca2+ uptake is achieved by synaptosomes preincubated for 0, 5 or 10 min. These findings suggest that Ca2+ uptake might depend on the Na-gradient formed during the preincubation of synaptosomes under control conditions.
Assuntos
Cálcio/metabolismo , Sódio/farmacologia , Sinaptossomos/metabolismo , Animais , Transporte Biológico Ativo/efeitos dos fármacos , Cinética , Masculino , Ratos , Ratos Endogâmicos , Sinaptossomos/efeitos dos fármacos
2.
Anal Biochem
; 90(1): 365-73, 1978 Oct 01.
Artigo
em Inglês
| MEDLINE
| ID: mdl-727475