[Mutagenic effects of coal tar used in the treatment of psoriasis]. / Mutagénne úcinky kamennouhol'ného dechtu pouzívaného pri liecbe psoriázy.

BACKGROUND: Pharmaceutic tar used in the treatment of psoriasis has a high contents of polycyclic aromatic carbohydrates which are considered to be carcinogenic. AIM: The study is aimed at the estimation of the genotoxic risk arising from the treatment of psoriatic patients by pharmaceutic tar. The estimation is performed by biomonitoring. METHODS: The used methods included: bacterial test on TA 98 strain of Salmonella typhimurium aimed at the assessment of mutagenity of urine and cytogenetic test aimed at the assessment of chromosomal aberrations in peripheral lymphocytes. RESULTS: The exposition of psoriatic patients to pharmaceutic tar significantly increased the index of mutagenity as an index of the presence of mutagen in urine, as well as the occurrence of chromosomal aberrations in peripheral lymphocytes. CONCLUSIONS: The results of monitoring have indicated that psoriatic patients treated by pharmaceutic tar are endangered by an increased genotoxic risk. (Tab. 2, Ref. 17.)

[Mutagenic activity of urine of patients with psoriasis treated with pharmaceutical black coal tar]. / Mutagénna aktivita mocu psoriatických pacientov liecených farmaceutickým kamennouhol'ným dechtom.

BACKGROUND: Examination of patients exposed for prolonged periods to black coal tar used in dermatology for the treatment of psoriasis revealed its carcinogenic effect. The objective of the present work was to assess the mutagenicity of urine samples from psoriatic patients treated with pharmaceutical black coal tar before treatment, and 14 and 28 days after application of the preparation. METHODS AND RESULTS: To a group of 19 patients with psoriasis vulgaris (12 men, mean age 43.3 +/- 3.96 years and 7 women, mean age 44.7 +/- 4.02 years) daily black coal tar in the form of Sack's tincture for a period of 28 days was applied to 10 - 80% of the body surface, i.e. 1000 - 13,000 cm2 of skin, amounting to 10 - 40 g. The mutagenic activity of urinary extracts was assessed by means of the Ames test on bacterial strain TA 98. It was revealed that urine samples from all patients exposed during the therapeutic procedure has a greater mutagenic activity, as compared with controls, whereby the mutagenicity index was higher in smokers (2.1) than in non-smoker patients (1.88) in the presence of the metabolic activating system. The elevated values of the mutagenicity index were recorder after the 14th day (2.25) and after the 28th day of treatment (2.07), as compared with controls (0.93); p < 0.05. Before the onset of treatment the mutagenicity index was 1.64. CONCLUSIONS: The assembled results confirm the view of an increased genotoxic risk in psoriatic patients treated with preparations containing black coal tar.