RESUMO
Abnormal decrease in microviscosity in erythrocytes cytosol during temperature decrease has been established when erythrocytes are in hypertonic sodium chloride solution in the presence of 1,2-propane diol and without it and also in the solution of polyethylene glycol-1500.
Assuntos
Eritrócitos/citologia , Citosol , Humanos , Concentração Osmolar , Polietilenoglicóis/química , Propilenoglicol , Propilenoglicóis/química , Cloreto de Sódio/química , Temperatura , ViscosidadeRESUMO
EPR spin-probe method was used to investigate the dependence of erythrocyte cytosol microviscosity (ECM) on the concentration of different substances and temperature. The critical ECM value has been determined after reaching of which erythrocytes become destroyed. It is shown that this ECM value is reached at different NaCl concentrations depending on the temperature.
Assuntos
Citosol/efeitos dos fármacos , Eritrócitos/efeitos dos fármacos , Espectroscopia de Ressonância de Spin Eletrônica , Ferricianetos/farmacologia , Humanos , Temperatura , Triacetonamina-N-Oxil , Viscosidade/efeitos dos fármacosRESUMO
Possibility of studying erythrocyte dehydration by ESR-spin probe is substantiated. Dehydration of erythrocytes in relation to osmolarity of sodium chloride solutions is investigated. The results are shown to agree with the data obtained by radioisotope method.
Assuntos
Líquidos Corporais/metabolismo , Eritrócitos/metabolismo , Líquido Intracelular/metabolismo , Dessecação , Espectroscopia de Ressonância de Spin Eletrônica , Humanos , Cinética , Marcadores de SpinRESUMO
It was shown by means of ESR-spin probe method that microviscosity of erythrocyte cytosol had a limiting value which could be reached by various concentrations of sodium chloride depending on temperature. With a temperature decrease when reaching this value haemolysis occurs.