Expert consensus on protocol of rehabilitation for COVID-19 patients using framework and approaches of WHO International Family Classifications.

Coronavirus disease 2019 (COVID-19) has widely spread all over the world and the numbers of patients and deaths are increasing. According to the epidemiology, virology, and clinical practice, there are varying degrees of changes in patients, involving the human body structure and function and the activity and participation. Based on the World Health Organization (WHO) International Classification of Functioning, Disability and Health (ICF) and its biopsychosocial model of functioning, we use the WHO Family of International Classifications (WHO-FICs) framework to form an expert consensus on the COVID-19 rehabilitation program, focusing on the diagnosis and evaluation of disease and functioning, and service delivery of rehabilitation, and to establish a standard rehabilitation framework, terminology system, and evaluation and intervention systems based the WHO-FICs.

The complete mitochondrial genome sequence of Margaritiana dahurica Middendorff.

In this study, the total mitochondrial genome of a freshwater pearl mussel Margaritiana dahurica Middendorff (Eulamellibranchia: Margaritanidae) was first determined. The genome is 16,112 bp in length. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 HORF. The overall nucleotide composition of the H-strand of M. dahurica Middendorff mitochondrial genome is A: 37.85%, G: 13.22%, T: 23.74% and C: 25.19%, respectively, with an A + T rich feature (61.59%). These results provide useful information to further study of the molecular systematics, species identification and conservation genetics of M. dahurica Middendorff.

LPS-induced TNFα factor (LITAF) in the snail Cipangopaludina chinensis: gene cloning and its apoptotic effect on NCI-H446 cells.

LPS-induced TNFα factor (LITAF) is a transcription factor mediating TNF-α expression under LPS stimulation, and playing important roles in immune responses. In the present study, partial cDNA sequence of a LITAF (designated CcLITAF) gene was cloned and identified from snail Cipangopaludina chinensis. It contains an open reading frame of 348 nucleotides encoding a predicted protein of 115 amino acids, with a conserved LITAF domain at C-terminal, and shares a similarity ranging from 34% to 96% with other LITAF from oyster to mammals. CcLITAF mRNA ubiquitously expressed in all analyzed tissues. Interestingly, cLITAF could induce apoptosis in human tumor cell line, NCI-H446 cells, and caspase 3 play key roles in CcLITAF-mediated apoptosis. Present studies provide new insight into the biological function of CcLITAF.