Evaluation of axial length/total corneal refractive power ratio as a potential marker for ocular diagnosis of Marfan's syndrome in children.

AIM: To investigate whether the axial length (AL)/total corneal refractive power (TCRP) ratio is a sensitive and simple factor that can be used for the early diagnosis of Marfan's syndrome (MFS) in children. METHODS: The relationship between the AL/TCRP ratio and the diagnosis of MFS for 192 eyes in 97 children were evaluate. The biological characteristics, including age, sex, AL, and TCRP, were collected from medical records. Receiver operating characteristic (ROC) curve analysis was performed to investigate whether the AL/TCRP ratio effectively distinguishes MFS from other subjects. The Youden index was used to re-divide the whole population into two groups according to an AL/TCRP ratio of 0.59. RESULTS: Of 96 subjects (mean age 7.46±3.28y) evaluated, 56 (110 eyes) had a definite diagnosis of MFS in childhood based on the revised Ghent criteria, 41 (82 eyes) with diagnosis of congenital ectopia lentis (EL) were included as a control group. AL was negatively correlated with TCRP, with a linear regression coefficient of -0.36 (R 2=0.08). A significant correlation was found between age and the AL/TCRP ratio (P=0.023). ROC curve analysis showed that the AL/TCRP ratio distinguished MFS from the other patients at a threshold of 0.59. MFS patients were present in 24/58 (41.38%) patients with an AL/TCRP ratio of ≤0.59 and in 34/39 (87.18%) patients with an AL/TCRP ratio of >0.59. CONCLUSION: An AL/TCRP ratio of >0.59 is significantly associated with the risk of MFS. The AL/TCRP ratio should be measured as a promising marker for the prognosis of children MFS. Changes in the AL/TCRP ratio should be monitored over time.

[The progress of proteomics approaches in cataract research].

Researches on lens proteomics are becoming prevalent in recent years. Characterized by their high resolution and efficiency, proteomics approaches provide reliable methodological support for lenses proteomic research, which demonstrates a new orientation for revealing the mechanism of cataractogenesis. This review outlines the separation, identification and some novel approaches of proteomic techniques applied in recent lenses proteomic research.

[Clinical study of diffuse lamellar keratitis after laser in situ keratomileusis].

OBJECTIVE: To investigate a new clinical grading scale of diffuse lamellar keratitis (DLK) following laser in situ keratomileusis (LASIK) and to observe the clinic outcomes of eyes treated with corresponding classified therapy. METHODS: It was a prospective case-control study. Eyes that developed DLK after LASIK were diagnosed by a new criterion of clinical grading scale based on both severity and scope of inflammatory cells infiltrations in the corneal interface. Dose and frequency of topical or systemic corticosteroid were determined by the classified profiles according to each grade. Clinical symptoms, corneal inflammatory cells infiltrations, visual acuity, refractive error and intraocular pressure (IOP) were evaluated on 1, 3, 5, 7, 10 days and 1 month after diagnosis as well as at the time of diagnosis. RESULTS: Among the 35 eyes of 29 patients that developed DLK, mild inflammatory cells infiltrations were mostly seen. Thirty eyes had degree I DLK and 3 had degree II DLK. One eye had degree III DLK and one had degree IV DLK. Inflammatory cells infiltrations in 20 eyes invaded peripheral zone (zone 1) and those in 11 eyes invaded mid-peripheral zone (zone 2). Inflammatory cells infiltrates in 4 eyes invaded central zone (zone 3). Nineteen eyes had degree I zone 1 DLK. Thirty three eyes were diagnosed between the 1st day and the 3rd day after surgery. Uncorrected visual acuity (UCVA) was worse than preoperative best spectacle corrected visual acuity (BSCVA). Inflammatory cells infiltrations retreated within 6.06 2.04 days after classified corticosteroid therapy. One month after the treatment, UCVA had improved to the level of preoperative BSCVA. BSCVA in 30 eyes were equal to or better than those before surgery. Spherical equivalent of the refractive error was (-0.21 +/- 1.16) D. There was no statistic difference in the retreat time of inflammatory cells infiltrations, recovery of visual acuity and refractive error among the different grades of DLK or among the DLK treated with different therapy profiles. No eye suffered from high intraocular pressure during the treatments. CONCLUSIONS: Clinical grading scale and corresponding classified therapy were proved to be effective in the treatment for DLK.