Radiation Oncology in the Philippines: Current State and Future Directions.

Purpose: As global cancer incidence continues to rise, low- to middle-income countries like the Philippines are projected to experience a disproportionate increase in cancer burden, further straining already limited resources. Radiation therapy (RT) is an essential and cost-effective modality in cancer care, both in the curative and palliative settings. In this article, we provide a brief narrative on the history of the field of radiation oncology in the Philippines and review the current challenges to effective and equitable RT service delivery in the country. Methods and Materials: We gathered data from the official websites of the Philippine government's health and statistics agencies, the Philippine Radiation Oncology Society, and the Directory of Radiotherapy Centers of the International Atomic Energy Agency, to review available human and infrastructure resources related to RT delivery in the country. Using the 6 health care dimensions of the World Health Organization's Building Blocks of Health Systems framework, we identified barriers to access and proposed possible initiatives for development. Results: Despite the remarkable growth of radiation oncology in the country in the past 2 decades, many challenges remain in the areas of human resources, infrastructure, policymaking, health economics, education, and service delivery. Radiation health workers and facilities are concentrated in the National Capital Region, limiting accessibility in rural areas. Out-of-pocket spending on RT-related expenditures remains high. The proper allocation of resources according to varying regional needs is impeded by the lack of a robust national cancer registry. Legislative reforms have been initiated but have yet to be fully implemented. Conclusions: Addressing these gaps in RT access will require in-depth study and multi-sectoral commitment aimed at establishing and implementing a nationwide framework for RT service delivery that can be readily adapted to varying regional needs. Despite many complex geographic, social, and economic obstacles, efforts by private and public sectors of society to provide ready access to RT services for all Filipinos continue to gain momentum.

Fractalkine isoforms differentially regulate microglia-mediated inflammation and enhance visual function in the diabetic retina.

Diabetic retinopathy (DR) affects about 200 million people worldwide, causing leakage of blood components into retinal tissues, leading to activation of microglia, the resident phagocytes of the retina, promoting neuronal and vascular damage. The microglial receptor, CX3CR1, binds to fractalkine (FKN), an anti-inflammatory chemokine that is expressed on neuronal membranes (mFKN), and undergoes constitutive cleavage to release a soluble domain (sFKN). Deficiencies in CX3CR1 or FKN showed increased microglial activation, inflammation, vascular damage, and neuronal loss in experimental mouse models. To understand the mechanism that regulates microglia function, recombinant adeno-associated viral vectors (rAAV) expressing mFKN or sFKN were delivered to intact retinas prior to diabetes. High-resolution confocal imaging and mRNA-seq were used to analyze microglia morphology and markers of expression, neuronal and vascular health, and inflammatory mediators. We confirmed that prophylactic intra-vitreal administration of rAAV expressing sFKN (rAAV-sFKN), but not mFKN (rAAV-mFKN), in FKNKO retinas provided vasculo- and neuro-protection, reduced microgliosis, mitigated inflammation, improved overall optic nerve health by regulating microglia-mediated inflammation, and prevented fibrin(ogen) leakage at 4 weeks and 10 weeks of diabetes induction. Moreover, administration of sFKN improved visual acuity. Our results elucidated a novel intervention via sFKN gene therapy that provides an alternative pathway to implement translational and therapeutic approaches, preventing diabetes-associated blindness.

Running Habits and Injury Frequency Following COVID-19 Restrictions in Adolescent Long-Distance Runners.

PURPOSE: A decline in youth running was observed at the start of the COVID-19 pandemic. We investigated whether the resumption of organized running after social distancing restrictions changed running habits or injury frequency in adolescent runners. METHODS: Adolescents (age = 16.1 [2.1] y) who participated in long-distance running activities completed an online survey in the Spring and Fall of 2020. Participants self-reported average weekly running habits and whether they sustained an injury during the Fall 2020 season. Poisson regression models and 1-way analysis of variance compared running habits while Fisher exact test compared differences in frequencies of injuries during Fall 2020 among season statuses (full, delayed, and canceled). RESULTS: All runners, regardless of season status, increased weekly distance during Fall 2020. Only runners with a full Fall 2020 season ran more times per week and more high-intensity runs per week compared with their Spring 2020 running habits. There were no differences in running volume or running-related injury frequency among Fall 2020 season statuses. CONCLUSIONS: There were no significant differences in running-related injury (RRI) frequency among runners, regardless of season status, following the resumption of cross-country. Health care providers may need to prepare for runners to increase running volume and intensity following the resumption of organized team activities.

Pre-pubertal runners demonstrate greater variability in running kinematics than post-pubertal runners.

BACKGROUND: Adolescents undergo a period of motor incoordination during puberty characterized by high movement variability. It is unknown if differences in running kinematics variability exist among adolescent long-distance runners. RESEARCH QUESTION: Is kinematic variability different among male and female adolescent long-distance runners of different stages of physical maturation? METHODS: We enrolled 114 adolescent long-distance runners (ages 8-19, F = 55, M = 59) in this secondary analysis of a larger cross-sectional study. Participants completed a three-dimensional overground running analysis at a comfortable self-selected speed. Peak frontal, sagittal, and transverse plane hip, knee, and ankle/shoe joint angles from the right leg were identified during stance phase for at least five trials. Variability in running kinematics was quantified as the standard deviation of the peak joint angles among the running trials for each participant. Participants were stratified by sex and stage of physical maturation (pre-, mid-, post-pubertal) and two-way ANOVAs compared between-subjects variability among groups (p ≤ .05). RESULTS: Significant sex by maturation interactions were observed for hip external rotation and ankle external rotation variability. Sex differences were observed for hip internal rotation, with males demonstrating greater variability, and ankle internal rotation, with females demonstrating greater variability. Pre-pubertal runners demonstrated significantly greater variability than mid-pubertal runners for hip flexion, and greater variability than post-pubertal runners for hip flexion, hip adduction, hip internal rotation, and knee flexion. SIGNIFICANCE: Pre-pubertal adolescent long-distance runners demonstrate greater stance phase variability in running kinematics than post-pubertal adolescent long-distance runners, while adolescent males and females demonstrate similar variability. Anthropometric and neuromuscular changes that occur during puberty likely influence running patterns and may contribute to more consistent kinematic patterns for post-pubertal runners.

Can Clinicians Trust Objective Measures of Hip Muscle Strength From Portable Dynamometers? A Systematic Review With Meta-analysis and Evidence Gap Map of 107 Studies of Reliability and Criterion Validity Using the COSMIN Methodology.

OBJECTIVE: To summarize the evidence on reliability and criterion validity of hip muscle strength testing using portable dynamometers. DESIGN: Systematic review with meta-analysis. LITERATURE SEARCH: Five databases were searched from inception to March 2023. STUDY SELECTION CRITERIA: We included studies investigating reliability or criterion validity of hip flexor, extensor, abductor, adductor, or internal/external rotator strength testing with portable dynamometers in injury-free individuals or those with pelvic/lower limb musculoskeletal disorders. DATA SYNTHESIS: We performed meta-analyses for each muscle group, position, and method of fixation. We rated pooled results as sufficient (>75% of studies with correlations ≥0.70), insufficient (>75% of studies with correlations <0.70), or inconsistent (sufficient/insufficient results). We assessed the quality of evidence, created evidence gap maps, and made clinical recommendations. RESULTS: We included a total of 107 studies (reliability 103, validity 14). The intrarater and interrater reliability for hip muscle strength testing across different positions and methods of fixation was sufficient (intraclass correlation coefficient = 0.78-0.96) with low- to high-quality evidence. Criterion validity was less investigated and mostly inconsistent (very low-to moderate-quality evidence) with a wide range of correlations (r = 0.40-0.93). CONCLUSION: Hip muscle strength testing using portable dynamometers is reliable. The use of portable dynamometers as clinical surrogates for measuring strength using an isokinetic dynamometer requires further investigation. Clinicians testing hip muscle strength with portable dynamometers should use external fixation seated for hip flexors, prone or supine for hip extensors, side-lying or supine for abductors and adductors, and prone and seated for internal and external rotators. J Orthop Sports Phys Ther 2023;53(11):655-672. Epub 3 October 2023. doi:10.2519/jospt.2023.12045.

Synthesis of a Novel Boronic Acid Transition State Inhibitor, MB076: A Heterocyclic Triazole Effectively Inhibits Acinetobacter-Derived Cephalosporinase Variants with an Expanded-Substrate Spectrum.

Class C Acinetobacter-derived cephalosporinases (ADCs) represent an important target for inhibition in the multidrug-resistant pathogen Acinetobacter baumannii. Many ADC variants have emerged, and characterization of their structural and functional differences is essential. Equally as important is the development of compounds that inhibit all prevalent ADCs despite these differences. The boronic acid transition state inhibitor, MB076, a novel heterocyclic triazole with improved plasma stability, was synthesized and inhibits seven different ADC ß-lactamase variants with Ki values <1 µM. MB076 acted synergistically in combination with multiple cephalosporins to restore susceptibility. ADC variants containing an alanine duplication in the Ω-loop, specifically ADC-33, exhibited increased activity for larger cephalosporins, such as ceftazidime, cefiderocol, and ceftolozane. X-ray crystal structures of ADC variants in this study provide a structural context for substrate profile differences and show that the inhibitor adopts a similar conformation in all ADC variants, despite small changes near their active sites.

Preferred temporal-spatial parameters, physical maturation, and sex are related to vertical and braking forces in adolescent long-distance runners.

For adults, increasing cadence reduces ground reaction forces, but a lower preferred cadence does not predispose adults to experience higher ground reaction forces. Pubertal growth and motor control changes influence running mechanics, but it is unknown if preferred cadence or step length are associated with ground reaction forces for pre-adolescent and adolescent runners. Pre-adolescent and adolescent runners underwent an overground running analysis at a self-selected speed. Mixed model multiple linear regressions investigated the associations of preferred cadence, step length, physical maturation, and sex on ground reaction forces, while accounting for running speed and leg length. Running with a lower preferred cadence or longer preferred step length was associated with larger peak braking and vertical forces (p ≤ .01), being less physically mature was associated with larger vertical impact peak force and vertical loading rate (p ≤ .01), and being a male was associated with larger loading rates (p ≤ .01). A lower preferred cadence or longer preferred step length were associated with higher braking and vertical forces and being less physically mature or a male were associated with higher loading rates. An intervention to increase cadence/decrease step length could be considered if ground reaction forces are a concern for an adolescent runner.

Sulfonamidoboronic Acids as "Cross-Class" Inhibitors of an Expanded-Spectrum Class C Cephalosporinase, ADC-33, and a Class D Carbapenemase, OXA-24/40: Strategic Compound Design to Combat Resistance in Acinetobacter baumannii.

Acinetobacter baumannii is a Gram-negative organism listed as an urgent threat pathogen by the World Health Organization (WHO). Carbapenem-resistant A. baumannii (CRAB), especially, present therapeutic challenges due to complex mechanisms of resistance to ß-lactams. One of the most important mechanisms is the production of ß-lactamase enzymes capable of hydrolyzing ß-lactam antibiotics. Co-expression of multiple classes of ß-lactamases is present in CRAB; therefore, the design and synthesis of "cross-class" inhibitors is an important strategy to preserve the efficacy of currently available antibiotics. To identify new, nonclassical ß-lactamase inhibitors, we previously identified a sulfonamidomethaneboronic acid CR167 active against Acinetobacter-derived class C ß-lactamases (ADC-7). The compound demonstrated affinity for ADC-7 with a Ki = 160 nM and proved to be able to decrease MIC values of ceftazidime and cefotaxime in different bacterial strains. Herein, we describe the activity of CR167 against other ß-lactamases in A. baumannii: the cefepime-hydrolysing class C extended-spectrum ß-lactamase (ESAC) ADC-33 and the carbapenem-hydrolyzing OXA-24/40 (class D). These investigations demonstrate CR167 as a valuable cross-class (C and D) inhibitor, and the paper describes our attempts to further improve its activity. Five chiral analogues of CR167 were rationally designed and synthesized. The structures of OXA-24/40 and ADC-33 in complex with CR167 and select chiral analogues were obtained. The structure activity relationships (SARs) are highlighted, offering insights into the main determinants for cross-class C/D inhibitors and impetus for novel drug design.

One size does not fit all: Influence of sex and maturation on temporal-spatial parameters for adolescent long-distance runners.

Runners and coaches are often interested in identifying the "ideal" running form to reduce the risk of injury and improve performance. While differences in pelvis and hip motion have been reported among adolescent female and male long-distance runners of different stages of physical maturation, the influence of sex and/or maturation on temporal-spatial parameters is unknown for adolescent runners. Adolescent runners of different stages of physical maturation (pre-, mid-, post-pubertal) completed an overground running analysis at a self-selected speed. We performed 2 × 3 ANCOVAs (covariate = running speed) to compare temporal-spatial parameters among sex and maturation groups. Pre-adolescents ran with higher cadences and shorter step lengths than mid- (p ≤ .01) and post-pubertal adolescents (p ≤ .01), respectively. Mid-pubertal males and post-pubertal females also ran with higher cadences and shorter step lengths than post-pubertal males (p ≤ .01). When step length was normalized to leg length, less physically mature runners demonstrated longer normalized step lengths (p ≤ .01). Caution is advised when using a "one-size-fits-all" approach for recommending an "ideal" cadence and/or step length for adolescent long-distance runners. A runner's sex, stage of physical maturation and leg length should be considered when assessing and prescribing cadence and/or step length.

Cadence in youth long-distance runners is predicted by leg length and running speed.

BACKGROUND: Lower cadence has been previously associated with injury in long-distance runners. Variations in cadence may be related to experience, speed, and anthropometric variables. It is unknown what factors, if any, predict cadence in healthy youth long-distance runners. RESEARCH QUESTION: Are demographic, anthropometric and/or biomechanical variables able to predict cadence in healthy youth long-distance runners. METHODS: A cohort of 138 uninjured youth long-distance runners (M = 62, F = 76; Mean ± SD; age = 13.7 ± 2.7; mass = 47.9 ± 13.6 kg; height = 157.9 ± 14.5 cm; running volume = 19.2 ± 20.6 km/wk; running experience: males = 3.5 ± 2.1 yrs, females = 3.3 ± 2.0 yrs) were recruited for the study. Multiple linear regression (MLR) models were developed for total sample and for each sex independently that only included variables that were significantly correlated to self-selected cadence. A variance inflation factor (VIF) assessed multicollinearity of variables. If VIF≥ 5, variable(s) were removed and the MLR analysis was conducted again. RESULTS: For all models, VIF was > 5 between speed and normalized stride length, therefore we removed normalized stride length from all models. Only leg length and speed were significantly correlated (p < .001) with cadence in the regression models for total sample (R2 = 51.9 %) and females (R2 = 48.2 %). The regression model for all participants was Cadence = -1.251 *Leg Length + 3.665 *Speed + 254.858. The regression model for females was Cadence = -1.190 *Leg Length + 3.705 *Speed + 249.688. For males, leg length, cadence, and running experience were significantly predictive (p < .001) of cadence in the model (R2 = 54.7 %). The regression model for males was Cadence = -1.268 *Leg Length + 3.471 *Speed - 1.087 *Running Experience + 261.378. SIGNIFICANCE: Approximately 50 % of the variance in cadence was explained by the individual's leg length and running speed. Shorter leg lengths and faster running speeds were associated with higher cadence. For males, fewer years of running experience was associated with a higher cadence.

Validity of an artificial intelligence, human pose estimation model for measuring single-leg squat kinematics.

Few studies have investigated the validity of 2D pose estimation models to evaluate kinematics throughout a motion and none have included adolescents. Adolescent athletes completed single-leg squats while 3D kinematic data and 2D sagittal and frontal plane videos were recorded. Sagittal and frontal plane joint motion throughout the single-leg squats and angles at peak knee flexion were compared among 2D pose estimation, 3D motion analysis, and traditional 2D motion analysis techniques. Statistical parametric mapping compared waveforms while Pearson's correlation compared the relationships of joint angles at peak knee flexion among techniques, respectively. We observed significant waveform differences between 2D pose estimation and 3D motion analysis at the beginning and end of the squat for sagittal plane hip and knee motion, for most of the squat for frontal plane hip motion, and throughout the entire squat for sagittal plane ankle motion and frontal plane pelvic motion. We observed moderate-to-strong relationships (r = 0.68-0.94) for sagittal plane joint angles between 2D pose estimation and 3D techniques. We observed fair correlations (r = 0.53-0.54) for frontal plane pelvic and hip joint angles between 2D pose estimation and 3D motion analysis. We observed poor relationships for the frontal plane knee angle between 3D motion analysis with 2D pose estimation (r = 0.20) and traditional 2D motion analysis (r = 0.05), respectively, but observed a strong relationship (r = 0.95) between the 2D techniques. 2D pose estimation is a valid alternative to 3D motion analysis and traditional 2D motion analysis for evaluating most sagittal and frontal plane angles during a single-leg squatting task.

Brachial plexus birth injury and cerebral palsy lead to a common contracture phenotype characterized by reduced functional muscle length and strength.

Introduction: Brachial plexus birth injury (BPBI) and cerebral palsy (CP) both cause disabling contractures for which no curative treatments exist, largely because contracture pathophysiology is incompletely understood. The distinct neurologic nature of BPBI and CP suggest different potential contracture etiologies, although imbalanced muscle strength and insufficient muscle length have been variably implicated. The current study directly compares the muscle phenotype of elbow flexion contractures in human subjects with BPBI and CP to test the hypothesis that both conditions cause contractures characterized by a deficit in muscle length rather than an excess in muscle strength. Methods: Subjects over 6 years of age with unilateral BPBI or hemiplegic CP, and with elbow flexion contractures greater than 10 degrees on the affected side, underwent bilateral elbow flexion isokinetic strength testing to identify peak torque and impulse, or area under the torque-angle curve. Subjects then underwent needle microendoscopic sarcomere length measurement of bilateral biceps brachii muscles at symmetric joint angles. Results: In five subjects with unilateral BPBI and five with hemiplegic CP, peak torque and impulse were significantly lower on the affected versus unaffected sides, with no differences between BPBI and CP subjects in the percent reduction of either strength measurement. In both BPBI and CP, the percent reduction of impulse was significantly greater than that of peak torque, consistent with functionally shorter muscles. Similarly, in both conditions, affected muscles had significantly longer sarcomeres than unaffected muscles at symmetric joint angles, indicating fewer sarcomeres in series, with no differences between BPBI and CP subjects in relative sarcomere overstretch. Discussion: The current study reveals a common phenotype of muscle contracture in BPBI and CP, with contractures in both conditions characterized by a similar deficit in muscle length rather than an excess in muscle strength. These findings support contracture treatments that lengthen rather than weaken affected muscles. Moreover, the discovery of a common contracture phenotype between CP and BPBI challenges the presumed dichotomy between upper and lower motor neuron lesions in contracture pathogenesis, instead revealing the broader concept of "myobrevopathy", or disorder of short muscle, warranting increased investigation into the poorly understood mechanisms regulating muscle length.

Mesowestern Blot: Simultaneous Analysis of Hundreds of Submicroliter Lysates.

Western blotting is a widely used technique for molecular-weight-resolved analysis of proteins and their posttranslational modifications, but high-throughput implementations of the standard slab gel arrangement are scarce. The previously developed Microwestern requires a piezoelectric pipetting instrument, which is not available for many labs. Here, we report the Mesowestern blot, which uses a 3D-printable gel casting mold to enable high-throughput Western blotting without piezoelectric pipetting and is compatible with the standard sample preparation and small (∼1 µL) sample sizes. The main tradeoffs are reduced molecular weight resolution and higher sample-to-sample CV, making it suitable for qualitative screening applications. The casted polyacrylamide gel contains 336, ∼0.5 µL micropipette-loadable sample wells arranged within a standard microplate footprint. Polyacrylamide % can be altered to change molecular weight resolution profiles. Proof-of-concept experiments using both infrared-fluorescent molecular weight protein ladder and cell lysate (RIPA buffer) demonstrate that the protein loaded in Mesowestern gels is amenable to the standard Western blotting steps. The main difference between Mesowestern and traditional Western is that semidry horizontal instead of immersed vertical gel electrophoresis is used. The linear range of detection is at least 32-fold, and at least ∼500 attomols of ß-actin can be detected (∼29 ng of total protein from mammalian cell lysates: ∼100-300 cells). Because the gel mold is 3D-printable, users with access to additive manufacturing cores have significant design freedom for custom layouts. We expect that the technique could be easily adopted by any typical cell and molecular biology laboratory already performing Western blots.

Global Positioning System Watches and Electronic Journals: Are Training-Load Measures Similar in High School Cross-Country Runners?

PURPOSE: Running programs are designed to progress training loads by manipulating the duration, frequency, and/or intensity of running sessions. While some studies use journals to monitor training load, others have used wearable technology. The purpose of this study was to compare the validity of self-reported and global positioning system (GPS)-watch-derived measures of external and internal loads in high school cross-country runners. METHODS: Twenty-two high school cross-country runners participated in the study during fall 2020. Participants recorded running sessions using a GPS watch and self-reported the running session using an electronic journal. External (distance and duration) and internal loads (session rating of perceived exertion [sRPE], average, and maximum heart rate) were retrieved from the GPS watch and electronic journal. Correlations compared relationships, and Bland-Altman plots compared agreements between GPS-watch-derived and self-reported measures of training loads. RESULTS: We found moderate relationships between self-reported and GPS-watch-derived measures of external loads (distance: r = .76, moving duration: r = .74, and elapsed duration: r = .70) and poor relationships between internal loads (sRPE vs average heart rate: ρ = .11, sRPE vs maximal heart rate: ρ = .13). We found mean differences of -0.8 km (95% = -6.3 to +4.8 km) for distance, -4.5 minutes (95% = -27.8 to +33.2 min) for moving duration, and 2.7 minutes (95% = -27.8 min to +33.2 min) for elapsed duration. CONCLUSIONS: High school runners overreported running distance and duration using self-reports, and self-reported and GPS-watch-derived measures of internal loads demonstrated poor agreement. Coaches and clinicians should use caution when comparing results from studies using different methods of monitoring training loads.

Risk of persistent and new clinical sequelae among adults aged 65 years and older during the post-acute phase of SARS-CoV-2 infection: retrospective cohort study.

OBJECTIVE: To characterize the risk of persistent and new clinical sequelae in adults aged ≥65 years after the acute phase of SARS-CoV-2 infection. DESIGN: Retrospective cohort study. SETTING: UnitedHealth Group Clinical Research Database: deidentified administrative claims and outpatient laboratory test results. PARTICIPANTS: Individuals aged ≥65 years who were continuously enrolled in a Medicare Advantage plan with coverage of prescription drugs from January 2019 to the date of diagnosis of SARS-CoV-2 infection, matched by propensity score to three comparison groups that did not have covid-19: 2020 comparison group (n=87 337), historical 2019 comparison group (n=88 070), and historical comparison group with viral lower respiratory tract illness (n=73 490). MAIN OUTCOME MEASURES: The presence of persistent and new sequelae at 21 or more days after a diagnosis of covid-19 was determined with ICD-10 (international classification of diseases, 10th revision) codes. Excess risk for sequelae caused by infection with SARS-CoV-2 was estimated for the 120 days after the acute phase of the illness with risk difference and hazard ratios, calculated with 95% Bonferroni corrected confidence intervals. The incidence of sequelae after the acute infection was analyzed by age, race, sex, and whether patients were admitted to hospital for covid-19. RESULTS: Among individuals who were diagnosed with SARS-CoV-2, 32% (27 698 of 87 337) sought medical attention in the post-acute period for one or more new or persistent clinical sequelae, which was 11% higher than the 2020 comparison group. Respiratory failure (risk difference 7.55, 95% confidence interval 7.18 to 8.01), fatigue (5.66, 5.03 to 6.27), hypertension (4.43, 2.27 to 6.37), memory difficulties (2.63, 2.23 to 3.13), kidney injury (2.59, 2.03 to 3.12), mental health diagnoses (2.50, 2.04 to 3.04), hypercoagulability 1.47 (1.2 to 1.73), and cardiac rhythm disorders (2.19, 1.76 to 2.57) had the greatest risk differences compared with the 2020 comparison group, with similar findings to the 2019 comparison group. Compared with the group with viral lower respiratory tract illness, however, only respiratory failure, dementia, and post-viral fatigue had increased risk differences of 2.39 (95% confidence interval 1.79 to 2.94), 0.71 (0.3 to 1.08), and 0.18 (0.11 to 0.26) per 100 patients, respectively. Individuals with severe covid-19 disease requiring admission to hospital had a markedly increased risk for most but not all clinical sequelae. CONCLUSIONS: The results confirm an excess risk for persistent and new sequelae in adults aged ≥65 years after acute infection with SARS-CoV-2. Other than respiratory failure, dementia, and post-viral fatigue, the sequelae resembled those of viral lower respiratory tract illness in older adults. These findings further highlight the wide range of important sequelae after acute infection with the SARS-CoV-2 virus.

Influence of hamstring flexibility on running kinematics in adolescent long-distance runners.

BACKGROUND: Studies have found no or minimal differences in running kinematics between flexible and inflexible adult runners. The interaction between hamstring flexibility and running kinematics has not been reported in adolescent long-distance runners. RESEARCH QUESTION: Does hamstring flexibility influence running kinematics in adolescent long-distance runners? METHODS: Adolescent long-distance runners (n = 140, ages 9-19) were enrolled in our cross-sectional study. Hamstring flexibility was assessed with the forward bending Beighton task. Runners were categorized if they tested positive or negative on the forward bending task. Participants ran at a comfortable self-selected speed on a treadmill. Runners who tested positive on the forward bending task (n = 17) were matched with runners who tested negative on the task (n = 17) according to sex, physical maturation, and running speed. Statistical parametric mapping compared trunk, pelvis, hip, and knee kinematic waveforms throughout the gait cycle and independent sample t tests compared temporal-spatial parameters between the groups. RESULTS: Runners who tested positive on the forward bending task demonstrated significantly greater anterior pelvic tilt during stance (average difference = 4.8° ± 0.4°, p < .001) and swing (average difference = 4.3° ± 0.2°, p < .01) compared to runners who tested negative on the forward bending task. No significant differences were found between groups for the remaining kinematic waveforms or for any temporal-spatial parameters (p > .05). SIGNIFICANCE: This is the first study to report the interaction between hamstring flexibility and running kinematics in adolescent long-distance runners. The greater anterior pelvic tilt demonstrated by runners with greater hamstring flexibility may place more eccentric demands on the hamstring musculature. However, as there were no other differences in joint kinematics or temporal-spatial parameters between groups, greater hamstring flexibility does not appear to have a significant interaction with running kinematics when running at sub-maximal speeds. Our results suggest hamstring flexibility does not predispose adolescent long-distance runners to sub-optimal segment positions associated with running-related injuries.

Acute changes in sagittal plane kinematics while wearing a novel belt device during treadmill running.

Somatosensory feedback is used in walking retraining; however, its utility in running is less feasible due to the greater associated speeds. The purpose of this study was to examine the acute effects of wearing a novel running belt device on sagittal plane running kinematics. Ten healthy runners ran on a treadmill with and without the use of a running belt device within a repeated measures study design. Temporal-spatial characteristics and sagittal plane knee and ankle kinematics were recorded with three-dimensional motion analysis. Wilcoxon Signed-Rank Tests revealed significant decreases in centre of mass vertical displacement (z = -2.083, p = 0.003), tibial inclination at initial contact (z = -2.803, p = 0.003), and stance phase knee joint excursion (z = -2.701, p = 0.003), and greater knee flexion at initial contact (z = -2.803, p = 0.003) when the belt was donned. No differences were observed in step rate (z = -0.351, p = 0.363), foot inclination angle at initial contact (z = -2.090, p = 0.018), or peak knee flexion during stance (z = -1.172, p = 0.121). Findings suggest that donning a running belt can minimise specific high-risk biomechanical characteristics in runners with particular kinematic profiles.

The influence of maturation and sex on pelvis and hip kinematics in youth distance runners.

OBJECTIVES: This study aimed to investigate differences in stance phase pelvic and hip running kinematics based on maturation and sex among healthy youth distance runners. DESIGN: Cross-Sectional. METHODS: 133 uninjured youth distance runners (M = 60, F = 73; age = 13.5 ±â€¯2.7 years) underwent a three-dimensional running analysis on a treadmill at a self-selected speed (2.8 ±â€¯0.6 m·s-1). Participants were stratified as pre-pubertal, mid-pubertal, or post-pubertal according to the modified Pubertal Maturational Observation Scale. Stance phase pelvis and hip range of motion (RoM) and peak joint positions were extracted. Two-way ANCOVAs (sex, maturation; covariate of running velocity) were used with Bonferroni-Holm method to control for multiple comparisons with a target alpha level of 0.05. RESULTS: A two-way interaction between sex and maturation was detected (p = 0.009) for frontal plane pelvic obliquity RoM. Post-hoc analysis identified a maturation main effect only among females (p˂0.008). Pelvic obliquity RoM was significantly greater among post-pubertal (p = 0.001) compared to pre-pubertal females. Significant main effects of sex (p = 0.02), and maturation (p = 0.01) were found for hip adduction RoM. Post-hoc analysis indicated a significant increase in hip adduction RoM from pre-pubertal to post-pubertal female runners (p = 0.001). A significant main effect of sex was found for peak hip adduction angle (p = 0.001) with female runners exhibiting greater maximum peak hip adduction compared to males. CONCLUSIONS: Maturation influences pelvic and hip kinematics greater in female than male runners. Sex differences became more pronounced during later stages of puberty. These differences may correspond to an increased risk for running-related injuries in female runners compared to male runners.

Quantification Method and Training Load Changes in High School Cross-Country Runners Across a Competitive Season.

CONTEXT: Running programs traditionally monitor external loads (eg, time and distance). Recent efforts have encouraged a more comprehensive approach to also account for internal loads (eg, intensity, measured as the session rating of perceived exertion [sRPE]). The combination of external and internal loads accounts for the possible interaction between these loads. Although weekly changes in training loads have been reported between external loads and the combination of external and internal loads during 2- and 4-week training cycles, no authors have indicated whether these differences occur during an entire cross-country season in high school runners. OBJECTIVE: To compare changes in training loads, as measured by (1) external loads and (2) combined external and internal loads in high school runners during an interscholastic cross-country season. DESIGN: Case series. SETTING: Community-based setting with daily online surveys. PATIENTS OR OTHER PARTICIPANTS: Twenty-four high school cross-country runners (females = 14, males = 10, age = 15.9 ± 1.1 years, running experience = 9.9 ± 3.2 years). MAIN OUTCOME MEASURE(S): Week-to-week percentage changes in training load were measured by external loads (time, distance) and combined external and internal loads (time × sRPE [timeRPE] and distance × sRPE [distanceRPE]). RESULTS: Overall, the average weekly change was 7.1% greater for distanceRPE than for distance (P = .04, d = 0.18). When the weekly running duration decreased, we found the average weekly change was 5.2% greater for distanceRPE than for timeRPE (P = .03, d = 0.24). When the weekly running duration was maintained or increased, the average weekly change was 10% to 15% greater when external and internal loads were combined versus external loads alone, but these differences were nonsignificant (P = .11-.22, d = 0.19-0.34). CONCLUSIONS: Progression in the training load may be underestimated when relying solely on external loads. The interaction between internal loads (sRPE) and external loads (distance or time) appears to provide a different measure of the training stresses experienced by runners than external loads alone.