RESUMO
Confirmation of the presence of Cryptosporidium in environmental samples is laborious, costly and often difficult. We report here a simple and economic slide agglutination test (co-agglutination test) for detecting cryptosporidial antigen in stool, serum and water. The results show that as a screening method co-agglutination is clearly superior to enzyme-linked immunosorbent assay (ELISA) and modified Ziehl-Neelsen staining, although ELISA is more accurate. The co-agglutination test is recommended for application as a new tool for detecting cryptosporidial antigen in large-scale epidemiological surveys.
Assuntos
Testes de Aglutinação/métodos , Antígenos de Protozoários/análise , Criptosporidiose/diagnóstico , Cryptosporidium parvum/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Doença Aguda , Testes de Aglutinação/normas , Animais , Antígenos de Protozoários/sangue , Antígenos de Protozoários/imunologia , Sangue/parasitologia , Estudos de Casos e Controles , Criança , Pré-Escolar , Doença Crônica , Criptosporidiose/sangue , Criptosporidiose/complicações , Criptosporidiose/imunologia , Criptosporidiose/parasitologia , Diarreia/parasitologia , Modelos Animais de Doenças , Egito , Ensaio de Imunoadsorção Enzimática/normas , Fezes/parasitologia , Humanos , Programas de Rastreamento/métodos , Programas de Rastreamento/normas , Camundongos , Sensibilidade e Especificidade , Água/parasitologiaRESUMO
Confirmation of the presence of Cryptosporidium in environmental samples is laborious, costly and often difficult. We report here a simple and economic slide agglutination test [co-agglutination test] for detecting cryptosporidial antigen in stool, serum and water. The results show that as a screening method co-agglutination is clearly superior to enzyme-linked immunosorbent assay [ELISA] and modified Ziehl-Neelsen staining, although ELISA is more accurate. The co-agglutination test is recommended for application as a new tool for detecting cryptosporidial antigen in large-scale epidemiological surveys
