[Concentration of glutathione (GSH), ascorbic acid (vitamin C) and substances reacting with thiobarbituric acid (TBA-rs) in single human brain metastases]. / Stezenie glutationu (GSH), kwasu askorbowego (witamina C) oraz substancji reagujacych z kwasem tiobarbiturowym (TBA-rs) w pojedynczych przerzutach nowotworowych do mózgu.

The aim of the study was to estimate the concentration of glutathione (GSH), ascorbic acid (vitamin C) and thiobarbituric acid (TBA-rs) in single human brain metastases and histologically unchanged nerve tissue. The research was conducted on fragments of neoplasmatic tissue collected from 45 patients undergoing surgery in the Department of Neurosurgery, Medical University of Bialystok in years 1996-2002. Concentration of GSH was evaluated using the GSH-400 method, vitamin C using the method of Kyaw and TBA-rs using the method of Salaris and Babs. It has been found that there is a decrease of concentration of GSH and vitamin C and a considerable increase (p < 0.05) of concentration of TBA-rs in investigated single brain human metastasis in correlation to the concentration of the mentioned above substances in unchanged nerve tissue.

Evaluation of antioxidant enzymes activity and concentration of non-enzymatic antioxidants in human brain tumours.

UNLABELLED: The purpose of our research was to assess the activity of GSH-Px, GSSG-R, SOD-1 and concentration of GSH, Vit.C and reactive substances with thiobarbituric acid, in brain tumours with Ist, IInd, IIIrd and IVth levels of biological malignancy. The research was conducted in 105 samples obtained from patients undergoing surgery in the Department of Neurosurgery of the Medical University of Bialystok between the years 1996-2001. The obtained values of the above enzyme activities and the concentration of the examined substances in brain tumours were compared to the control values determined in 15 samples of histopathology unchanged nerve tissue. THE RESULTS: The increase in all enzymes activities in brain tumours is statistically significant (p < 0.05) as compared to the control level in normal nerve tissue alto a significant decrease (p < 0.05) of the GSH and ascorbate concentration and a significant increase (p < 0.05) in the level of thiobarbituric acid-reactive substances in the examined brain tumours was found in comparison to the concentration of the above substances in normal nerve tissue.

[Activity of glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R) and superoxide dismutase (SOD-1) in single brain metastasis]. / Aktywnosc peroksydazy glutationowej (GSH-Px), reduktazy glutationowej (GSSG-R) i dysmutazy nadtlenkowej (SOD-1) w pojedynczych przerzutach nowotworowych do mózgu.

The aim of the study was to evaluate the activity of glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R) and superoxide dysmutase (SOD-1) in the single brain metastases. The activity of the GSH-Px was evaluated with the use of spectrophotometry, GSSG-R was evaluated basing on the method of Mize and Langdon and SOD-1 with Sykes et al. method. The examinations were carried out in 36 specimens (10 specimens of healthy brain tissue, 12 specimens of brain metastases, 14 specimens of glioma multiforme). The statistical analysis revealed significant increase (p < 0.001) of GSH-Px and GSSG-R activity within the single brain metastases in comparison with the healthy brain tissue.

[Evaluation of surgical treatment results in extreme lateral lumbar disc prolapsed after 12 months of follow-up]. / Ocena wyników chirurgicznego leczenia krancowobocznie wypadnietego jadra miazdzystego w ledzwiowej czesci kregoslupa po dwunastomiesiecznym okresie obserwacji pooperacyjnej.

In 1988-1997, patients with extreme lateral disc prolaps (KBWJM) were operated on the Department of Neurosurgery Medical Academy of Bialystok. Females accounted for 4.9% (2 cases) and 95.1% were males (39 cases) with age range 41-60 years. During a study of clinical state 12 months after the operation, we found in 75.6% of patients good and very good results of the surgical treatment of KBWJM. During that period of time, two patients (4.9%) were reoperated out of 41 all patients.

The decrease in antioxidant potential in human brain tumours.

UNLABELLED: The objective of our research was to estimate the activity of superoxide dismutase (SOD-1), glutathione peroxidase (GSH-Px) and glutathione reductase (GSSG-R), as well as the concentrations of free oxygen radicals "sweepers" (GSH, Vit.C) and the concentrations of the substances reacting with thiobarbituric acid in brain tumours with II, III and IV level of biological malignancy. The research was conducted on 61 samples obtained from the patients operated in the Department of Neurosurgery of the Medical Academy of Bialystok between the years 1996 and 2000. The obtained values of the above enzyme activities and of the concentrations of the examined substances in brain tumours were compared to the adequate values determined in 10 samples of histopathologically unchanged nerve tissue. THE RESULTS: the increase of all enzymes activities in brain tumours is statistically significant (p < 0.05) as compared to the adequate activity level in normal nerve tissue. The significant decrease (p < 0.05) of the GSH and ascorbate concentration and the significant increase (p < 0.05) of the level of the thiobarbituric acid--reactive substances in the examined brain tumours was found in comparison to the adequate concentration of the above substances in normal nerve tissue.