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Anim Reprod Sci ; 196: 230-238, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30115520

RESUMO

The key prerequisite for successful insemination is sperm characterized to have positive values for morphological and biological variables which are determined by, among others, effective antioxidant protection during the lifespan of sperm cells. This study evaluated the activity and relative abundance of mRNA for antioxidant enzymes in stallion testicular and epididymal tissues during breeding (n = 5) and non-breeding (n = 5) seasons. The activity of superoxide dismutase (SOD) was greater (P < 0.05) during the breeding season, in particular in the testes and the caput epididymis, and SOD1 was the predominant isoform of the enzyme. The expression of the SOD3 gene was markedly less in the analyzed tissues, which indicates that this enzyme contributes to the antioxidant protection of the stallion reproductive tract. The activity of catalase (CAT) was less (P < 0.05) in the testes during both seasons while its relative abundances only during the breeding season. The greatest CAT activity was noted in the cauda epididymis during the breeding season. The activity of glutathione peroxidases (GPx) was greater (P < 0.05) in the testes than in other tissues and 10-fold greater during the breeding season. Similarly, relative abundance of GPx5 mRNA was greater (P < 0.05) in the caput epididymis than in the remaining tissues during both seasons. This study demonstrated that season has an ambiguous influence on the antioxidant defense system in stallion reproductive tissues. Seasonal differences in the present study, however, indicate that the reproductive system of stallions adapts well to environmental seasonal changes.


Assuntos
Cruzamento , Epididimo/enzimologia , Cavalos , RNA Mensageiro/metabolismo , Testículo/enzimologia , Animais , Masculino , Estações do Ano , Espermatozoides
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