Gas Fermentation-A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks.

There is an immediate need to drastically reduce the emissions associated with global fossil fuel consumption in order to limit climate change. However, carbon-based materials, chemicals, and transportation fuels are predominantly made from fossil sources and currently there is no alternative source available to adequately displace them. Gas-fermenting microorganisms that fix carbon dioxide (CO2) and carbon monoxide (CO) can break this dependence as they are capable of converting gaseous carbon to fuels and chemicals. As such, the technology can utilize a wide range of feedstocks including gasified organic matter of any sort (e.g., municipal solid waste, industrial waste, biomass, and agricultural waste residues) or industrial off-gases (e.g., from steel mills or processing plants). Gas fermentation has matured to the point that large-scale production of ethanol from gas has been demonstrated by two companies. This review gives an overview of the gas fermentation process, focusing specifically on anaerobic acetogens. Applications of synthetic biology and coupling gas fermentation to additional processes are discussed in detail. Both of these strategies, demonstrated at bench-scale, have abundant potential to rapidly expand the commercial product spectrum of gas fermentation and further improve efficiencies and yields.

Carbon recovery by fermentation of CO-rich off gases - Turning steel mills into biorefineries.

Technological solutions to reduce greenhouse gas (GHG) emissions from anthropogenic sources are required. Heavy industrial processes, such as steel making, contribute considerably to GHG emissions. Fermentation of carbon monoxide (CO)-rich off gases with wild-type acetogenic bacteria can be used to produce ethanol, acetate, and 2,3-butanediol, thereby, reducing the carbon footprint of heavy industries. Here, the processes for the production of ethanol from CO-rich off gases are discussed and a perspective on further routes towards an integrated biorefinery at a steel mill is given. Recent achievements in genetic engineering as well as integration of other biotechnology platforms to increase the product portfolio are summarized. Already, yields have been increased and the portfolio of products broadened. To develop a commercially viable process, however, the extraction from dilute product streams is a critical step and alternatives to distillation are discussed. Finally, another critical step is waste(water) treatment with the possibility to recover resources.

2,3-butanediol production by acetogenic bacteria, an alternative route to chemical synthesis, using industrial waste gas.

2,3-Butanediol (23BD) is a high-value chemical usually produced petrochemically but which can also be synthesized by some bacteria. To date, the best microbial 23BD production rates have been observed using pathogenic bacteria in fermentation systems that depend on sugars as the carbon and energy sources for product synthesis. Here we present evidence of 23BD production by three nonpathogenic acetogenic Clostridium species-Clostridium autoethanogenum, C. ljungdahlii, and C. ragsdalei-using carbon monoxide-containing industrial waste gases or syngas as the sole source of carbon and energy. Through an analysis of the C. ljungdahlii genome, the complete pathway from carbon monoxide to 23BD has been proposed. Homologues of the genes involved in this pathway were also confirmed for the other two species investigated. A gene expression study demonstrates a correlation between mRNA accumulation from 23BD biosynthetic genes and the onset of 23BD production, while a broader expression study of Wood-Ljungdahl pathway genes provides a transcription-level view of one of the oldest existing biochemical pathways.

Fermentative production of ethanol from carbon monoxide.

'Too much Carbon Monoxide for me to bear…' are the opening lyrics of the CAKE song Carbon Monoxide (from their 2004 album Pressure Chief), and while this may be the case for most living organisms, several species of bacteria both thrive on this otherwise toxic gas, and metabolize it for the production of fuels and chemicals. Indeed CO fermentation offers the opportunity to sustainably produce fuels and chemicals without impacting the availability of food resources or even farm land. Mounting commercial interest in the potential of this process has in turn triggered greater scrutiny of the molecular and genetic basis for CO metabolism, as well as the challenges associated with the implementation and operation of gas fermentation at scale.

Focusing characteristics of a planar solid-immersion mirror.

The focusing characteristics of a planar waveguide solid-immersion mirror with parabolic design have been investigated. The solid-immersion mirror is integrated into an optical waveguide, and light focusing is achieved with a parabolic mirror parallel to the waveguide plane and waveguide mode confinement normal to the waveguide plane. Optical-quality tantala silica planar waveguides can be obtained by evaporation. The parabolic sidewall reflects over 50% of the incident waveguide mode and generates a diffraction-limited focus. The measured spot size for the solid-immersion mirror described here is less than one third of the wavelength. Polarization analysis shows that the electric field near the focal region has components parallel and normal to the polarization state of the incident beam. The planar solid-immersion mirror is essentially free of chromatic aberration, and the alignment of the illumination beam is within a fraction of degrees.

Miniature planar solid immersion mirror with focused spot less than a quarter wavelength.

We describe a microoptical planar waveguide solid immersion mirror with high optical throughput, and show that it can focus light to spot sizes of ~90 nm at a wavelength of 413 nm. Scanning near field optical microscope images of the light within the device are in good agreement with a simple theoretical model. This device is accurately mass-produced with lithographic and thin film deposition techniques known from modern integrated circuit processing.