1.
Plant Cell Rep
; 8(4): 247-50, 1989 Apr.
Artigo
em Inglês
| MEDLINE
| ID: mdl-24233148
RESUMO
Protoplasts were isolated from hypocotyls of etiolated seedlings from a diploid and the corresponding autotetraploid variety of common buckwheat (Fagopyrum esculentum). The isolated protoplasts started to divide after 4 days in culture in a modified MS medium. Maximum plating efficiency was approximately 1%. Regenerated calli derived from the tetraploid genotype developed roots easily but were recalcitrant to form shoots. Eighteen months following the initiation of cultures, tetraploid embryoids and shoots emerged after 3 weeks on an MS medium containing 0.1 mg/l gibberellic acid.
2.
Jpn Circ J
; 42(1): 63, 1978 Jan.
Artigo
em Inglês
| MEDLINE
| ID: mdl-633603