[Yield of Chlamydia from an infected cell studied by scanning electron microscopy]. / Izuchenie vykhoda khlamidii iz infitsirovannoi kletki metodom skaniruiushchei élektronnoi mikroskopii.

The study of the monolayer cell culture L-929, infected with strain CP-1 isolated from a patient with Reiter's syndrome, by means of scanning electron microscopy demonstrated that the release of chlamydiae from infected cells occurred in two ways: (1) by active ejection, probably as the consequence of the local rupture of the host cell, and (2) by more quiet "eruption" due to rupture in the plasmolemma . Elementary chlamydial bodies released from the cell had a spherical form, and reticular bodies had an irregularly spherical form, often with recesses and digitiform protrusions.

[Characteristics of the lymphoid cells of rabbits]. / K kharakteristike limfoidnykh kletok krolika.

By means of scanning electron microscopy it has been stated that the thymus gland, spleen, paratracheal lymph node, Peyer's patches and appendix in an intact rabbit of "Shinshilla" strain have T- and B-lymphocytes in different proportion. T-lymphocytes are seen as spherical cells with rough surface, with a few microprocesses, have 3.0 +/- 0.1 mcm--4.7 +/- 0.2 mcm in diameter depending on their location. B-lymphocytes are seen as spherical cells with a considerable amount of microvilli on their surface, have 2.9 +/- 0.1 mcm--4.4 +/- 0.1 mcm in diameter depending also on their location. In the thymus gland, cells with rough surface, a few microprocesses and crater-like hollows prevail. In the spleen and lymph node, most of the cells are of similar diameter and have microvilli on the surface. Lymphoid population in the Peyer's patch and appendix is represented by large cells with microprocesses.

[Study of streptococcal L forms in the scanning electron microscope. II. Dynamics of the development of structural elements of L colonies at different stages of growth]. / Izuchenie L-form streptokokka v skaniruiushchem élektronnom mikroskope. Soobshchenie II. Dinamika razvitiia strukturnykh élementov L-kolonii na raznykh stadiiakh rosta

The method of scanning electron microscopy showed that the L-colonies of streptococcus were formed by the spherical structures 0.1--1.5 micronm in diameter, elements of polygonal shape (large bodies) 10--30 micronm in size, filamentous structures 01--7 micronm in diameter and structureless matrix. A regular replacement of one form by another was observed in the process of the L-colonies development. Thus, the spherical elements appeared in the lag-phase, and polygonal elements were found mostly at the initial stages of the L-colonies formation; as to the filamentous structures -- they were present at all the developmental stages, but their diameter increased, and their structure and number changed at different growth phases. The spherical elements of the L-colonies formed evenly both on the structureless depth matrix of the colonies, on the filamentous structures in the form of buds on the "large bodies", and the disintegration of the latter. The role of the filamentous structures in the development of the L-colonies is discussed.

[A study of Proteus L forms during reversion by means of electron microscopy and ultrathin sections]. / Izuchenie L-form protea v protsesse reversii metodom skaniruiushchei élektronnoi mikroskopii i ul'tratonkikh srezov

The method of scanning electron microscopy was applied to the study of the Proteus L-forms which reversed directly on the millipore filters. It appeared that reversion was accompanied by elongation of the cell, piriform cells forming as the result. Filamentous cells segmented into short fragments and large bodies with a spongy surface structure with numerous ruptures were also revealed in the reversing culture. The method of ultrathin sections showed the other membrane in many cells to be fragile and readily twisting forming vesicles collecting into chains. As supposed, this is connected with degenerative changes involving the outer membrane of the paternal L-forms at the early reversion periods. An interrupted structure of the outer membrane was also demonstrated in many cells. It is supposed that in this case a synthesis of the outer membrane de novo at the later stages of reversion is dealt with. Phenomenon of "extrusion" of the multilayer structures into the periplasmic space, characteristic of spheroplasts, is described. A marked similarity of the morphological picture of reversion and at the initial stages of L-transformation, i.e. of the processes representing a looking glass reflection of one another, is described.

[L-forms of streptococcus in the scanning electron microscope. 1. Structural elements of L-colonies]. / L-formy streptokokka v skaniruiushchem élektronnom mikroskope. Soobshchenie 1. O strukturnykh élementakh L-kolonii

Broth culture of stable L-forms of beta-hemolytic streptococcus, group A, was studied in the log phase. Internal portion of the centrifugate constituting the matrix of the L-population represented a structureless mass in which the borders of individual cells were not always distinct; the cells adhered to one another and were irregular in shape. The described morphological picture corresponded to the internal part of the L-colonies from the semifluid medium in observation under phase and scanning microscope. A more peripheral portion of the centrifugate was formed by the spherical cells, about 2 mum in diameter; penetrating holes or crater-like invaginates and also small spherical structures, about 0.5 mum in diameter were often revealed on these cells. Filamentous structures, up to 200 mum in length which protruded far into the surrounding environment served as the most peripheral elements. Analogous structure were described in the L-colonies examined by phase contrast microscopy. The data of transmission and scanning microscopy are compared.