[New screening method for aneuploidies based on analysis of cell-free DNA in the maternal blood].

UNLABELLED: The national program of screening for aneuploidies in Bulgaria is based on first trimester combined test, second trimester biochemical test and/or the combination between first and second trimester integrated test. OBJECTIVE: To review the literature for studies analyzing cell-free (cf) DNA in the maternal blood and to report the clinical implementation and validation of the method in the clinical practice. Literature search and study selection extracted studies since 2011 when the first article was published. The data source included searches from PubMed and Medline. The reported results for detection rates (DR) and false positive rates (FPR) in singleton pregnancies were about 99.0% and 0.08% respectively, for trisomy 21, 96.8% and 0.15% for trisomy 18, 92.1% and 0.20% for trisomy 13, 88.6% and 0.12% for monosomy X. For twin pregnancies, the DR was 94.4% and FPR was 0% for trisomy 21. CONCLUSION: Analysis of cell-free DNA in the maternal blood is an effective method of screening for aneuploidies.

[Assesment of the woman's age as a main predictive factor of assisted reproductive technology treatment outcome].

Several factors could be used as a predictors of in vitro fertilization treatment outcome: female age, aetiology of sterility, previous ovarian surgery, embryo morphology and cleavage rates and also the technique of embryo transfer. The aim of the present study is to evaluate the role of the age to be used as a prognostic factor for the sterility treatment outcome. The tasks we assigned were to compare the several parameters: duration of the stimulation, number of retrieved oocytes, fertilization rate, implantation rate and clinical pregnancy rate after IVF/ICSI in different age's groups. The study results clearly demonstrate that in spite of decreasing number of retrieved oocytes after the age of 30, the fertilization rate remains elevated till 40 years. Regardless of this fact, the implantation rate and the clinical pregnancy rate were significantly lower after the age of 35. Reduction of the success rate in these cases showed that ART, despite of its renewals, is not the strategy for getting over the age in human reproduction.