RESUMO
Sixty-three sera of patients with leprosy were tested for the presence of circulating immune complexes (CIC) by C1q solid phase assay (C1qSPA). The mean values in CIC levels in leprosy patients were very high in comparison to native and European controls. No difference was found in the tuberculoid and lepromatous forms, but there was a good correlation between the presence of CIC and autoantibodies.
Assuntos
Complexo Antígeno-Anticorpo/análise , Hanseníase/imunologia , Adolescente , Adulto , Idoso , Complexo Antígeno-Anticorpo/imunologia , Enzimas Ativadoras do Complemento/análise , Complemento C1q , Feminino , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
The polyethylene-glicol (P.E.G.) precipitation assay was used to examine the sera of 91 pregnant women, 30 with normal pregnancies, 16 with EPH-Gestosis, 20 with pregnancy complicated by diabetes and 25 with case histories of recurrent abortions, in order to find evidence of eventual circulating immune complexes (C.I.C.). We also examined 30 amniotic fluids from normal pregnant women and 6 from pregnant women with diabetes. Several sera and all amniotic fluids were also examined by rheumatoid factor (RF) - inhibition test. C.I.C. were not discovered in the normal pregnant women neither in their sera nor in their amniotic fluids, while they were found in the following cases: in 8 patients with EPH-Gestosis (50%), in 8 with diabetes (40%) and in 3 with anamnesis positive for recurrent abortions (12%). The 6 amniotic fluid samples from women with diabetes were all negative. We were only able to examine 11 of the 19 positive cases from 4 days to 5 months after delivery and C.I.C. were absent in the sera of all the patients.
Assuntos
Líquido Amniótico/imunologia , Complexo Antígeno-Anticorpo/análise , Pré-Eclâmpsia/imunologia , Gravidez em Diabéticas/imunologia , Gravidez , Adulto , Complexo Antígeno-Anticorpo/sangue , Diabetes Mellitus/imunologia , Feminino , Humanos , MasculinoRESUMO
The polyethylenglycol (PEG) precipitation technique has been employed for the measurement of immune complexes in the circulation of 100 normal subjects and in 14 patients suffering from a variety of diseases (systemic lupus erythematodes, nephrosic syndrome, cryoglobulinaemia, Buckley's syndrome). Values higher than 0.80 UA on the absorption scale were considered pathological, namely 2 standard deviations above the mean (0.32 UA) in the subjects examined; in the patients, values between a minimum of 0.98 UA and a maximum of 2.38 UA were observed. Longitudinal study of these cases also pointed to the disappearance of immune complexes during therapy. The results suggest that the PEG precipitation technique can play an important part as a screening test in situations in which circulating IC pathology is suspected; it is also a sensitive means of monitoring treatment.
