Relation of changes in dietary lipids and weight, trial years 1-6, to changes in blood lipids in the special intervention and usual care groups in the Multiple Risk Factor Intervention Trial.

For men in the special intervention (SI) group of the Multiple Risk Factor Intervention Trial, the average decrease in serum total cholesterol was 16.9 mg/dL (6.7%); for men in the usual care (UC) group, the average decrease was 9.7 mg/dL (3.8%). The difference between the two groups for plasma total cholesterol was 6.2 mg/dL. Plasma low-density-lipoprotein (LDL) cholesterol decreased 10.6 mg/dL (6.6%) in SI men and 5.4 mg/dL (3.4%) in UC men. Mean weight losses were 3.0 lb (1.36 kg) and 0.1 lb (0.05 kg) for SI and UC men, respectively. Change in blood total cholesterol was directly related to baseline concentration; for men with serum total cholesterol > or = 220 mg/dL, those in the SI group decreased their total cholesterol by 7.8% (design goal: 10%) and those in the UC group by 4.8% (expected: 0%). Change in dietary lipid intake (summarized by the Keys score) for SI men was significantly related to changes in blood total cholesterol, LDL cholesterol, and triglyceride, but not to change in high-density-lipoprotein (HDL) cholesterol. Controlled for weight change, coefficients for Keys score change were smaller but remained significantly related to each blood lipid except HDL cholesterol. Weight loss was associated with favorable effects on all blood lipids. Influences of change in diet and weight on blood lipids were quantitatively less for hypertensive men for serum total cholesterol, HDL cholesterol, and triglyceride than for nonhypertensive men. Nonsmokers had greater decreases than smokers in blood total cholesterol, LDL cholesterol, and triglyceride.

S-100 protein and neuron specific enolase immunoreactivity of normal, hyperplastic and neoplastic chondrocytes in relation to the composition of the extracellular matrix.

S-100 protein and neuron specific enolase (NSE) are no longer considered as specific cell markers indicating a neural origin. Since most of the cells displaying immunoreactivity for both markers also elaborate a stroma rich in chondroid or myxoid mucosubstances, we undertook the present study in order to clarify whether or not the positive immunoreaction is related to the composition of stromal glycosaminoglycans. The study was based on formalin fixed, paraffin embedded material comprising adult resting cartilage, reactive or hyperplastic cartilage, as well as benign and malignant chondroblastic tumors. Histochemical and immunohistochemical methods were applied on parallel sections with the following results: A positive immunoreactivity of the cartilage cells was always found to be related to the participation of chondroitine sulfate A and C in the stromal glycosaminoglycans. A NSE positive reaction was found in all cartilage cells displaying the characteristics of metabolically active cells. It is postulated that S-100 protein, as a calcium binding protein, might be involved in the cellular control mechanisms regulating the glycosaminoglycans-collagen interactions.

S-100 protein and neuron specific enolase (NSE) expression by chordomas in relation to the composition of their stromal mucosubstances.

The immunoreactivity of S-100 protein and neuron specific enolase (NSE.) was correlated with the composition of stromal glycosaminoglycans in chordomas and human notochords, in a combined histochemical and immunohistochemical study. We found that S-100 protein is negative in notochordal and chordoma cells in the absence of stromal mucosubstances or in the presence of small quantities of hyaluronic acid. The positivity of S-100 immunoreaction was found to be related to the presence of stromal glycosaminoglycans of the chondroitine sulfate A and C type. NSE. was found positive in cells presenting features of high metabolic activity. Consequently S-100 protein and NSE. immunoreactivity cannot have any cytogenetic implications, but they could be considered as markers indicating specific cell-stromal functional interactions.