RESUMO
Ethanol-soluble, but saline-insoluble antigens were prepared as saline suspensions and studied in double diffusion reactions in a soft agarose gel. Positive reactions were observed with syphilis and SLE sera tested against the Kahn antigen as well as against commercial cardiolipin reagents. Also, ethanol-soluble brain antigen was studied for organ-specific reactions with rabbit immune sera. It was shown that double diffusion in gel can be employed as an analytical procedure for studies on reactions of saline suspensions of ethanol-soluble antigens.
Assuntos
Anticorpos Antibacterianos/sangue , Reações Antígeno-Anticorpo , Antígenos de Bactérias/química , Antígenos de Bactérias/imunologia , Encéfalo/imunologia , Animais , Anticorpos Antibacterianos/imunologia , Antígenos de Bactérias/metabolismo , Cardiolipinas/imunologia , Bovinos , Meios de Cultura , Etanol , Testes de Floculação , Humanos , Imunodifusão , Especificidade de Órgãos , Coelhos , Solubilidade , Suínos , Sífilis/diagnóstico , Sífilis/imunologiaRESUMO
Previous studies on the appearance in pathological human sera of antibodies to an antigen of normal mammalian organs were continued. In gel precipitation reactions, antibodies combining with saline extracts of mammalian organs were found in 20 of 63 cancer sera and in 4 of 15 syphilis sera, but only in 3 of 56 other pathological sera. Furthermore, an identical antigen was demonstrated in 5 of 58 pathological sera. The antigen under study did not belong to any group of known heterophile antigens and it was devoid of organ and tissue specificity.
Assuntos
Antígenos de Neoplasias/imunologia , Antígenos/imunologia , Neoplasias do Colo/imunologia , Sífilis/imunologia , Animais , Anticorpos Heterófilos/imunologia , Antígenos de Neoplasias/sangue , Bovinos , Neoplasias do Colo/sangue , Humanos , Imunodifusão , Fígado/imunologia , Sífilis/sangue , Sorodiagnóstico da Sífilis , Extratos de Tecidos/imunologiaRESUMO
INTRODUCTION: Previous numerous papers by the senior author dealt with the human serum factor referred to as anti-antibody which is specifically directed against IgG antibodies that underwent molecular transformation in the course of the reactions with their corresponding antigens. The reactions of this serum factor could be conveniently detected by means of agglutination of Rh-positive erythrocytes sensitized by anti Rh antibodies. No precipitation tests could be developed. MATERIAL AND METHODS: Most studies were conducted by means of double diffusion in gel precipitation. RESULTS: A rheumatoid arthritis serum, G, was noted that produced a strong reaction of double diffusion in gel precipitation with serum samples of a renal graft recipient, T. Further screening detected one more rheumatoid arthritis serum reacting with T; of 28 sera from renal graft recipients, 6 reacted in a similar way to T, but the reactions were considerably weaker and poorly reproducible. Evidence was presented that the precipitin in the two rheumatoid arthritis sera under study had properties of previously described anti-antibody. CONCLUSIONS: Sera with precipitating anti-antibodies may serve as exquisite reagents for detection of soluble immune complexes in human sera.