RESUMO
Molecular cloning, nucleotide sequencing, and characterization of the flaA gene from additional isolates of urease-positive thermophilic Campylobacter (UPTC) were performed. These isolates were obtained from the natural environment in Northern Ireland (n = 9 from mussels) and in England (n = 1 from sea water). All isolates carried the shorter flaA gene, [open reading frames (ORFs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaA pseudogenes. The UPTC isolates were well discriminated by the neighbor joining (NJ) phylogenetic tree constructed based on the putative flaA genes ORFs nucleotide sequence information. In addition, the NJ tree constructed based on the flaA-short variable region sequence information discriminated the Campylobacter lari isolates with a similar degree of discrimination power.
Assuntos
Bivalves/microbiologia , Campylobacter/genética , Flagelina/genética , Proteínas Recombinantes/genética , Água do Mar/microbiologia , Análise de Sequência de DNA/métodos , Sequência de Aminoácidos , Animais , Campylobacter/classificação , Campylobacter/isolamento & purificação , Campylobacter/metabolismo , Campylobacter lari/classificação , Campylobacter lari/genética , Campylobacter lari/isolamento & purificação , Campylobacter lari/metabolismo , Clonagem Molecular , Inglaterra , Flagelina/química , Flagelina/metabolismo , Dados de Sequência Molecular , Irlanda do Norte , Fases de Leitura Aberta , Filogenia , Reação em Cadeia da Polimerase , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Urease/metabolismoRESUMO
A case is presented of infective endocarditis caused by a previously undescribed Helcococcus-like organism, in a patient with human immunodeficiency virus infection. The organism, which could not be identified phenotypically in the routine laboratory, was identified using partial 16S rRNA gene sequencing.