Influence of stathmin 1 (STMN1) expression on neoangiogenesis in colorectal adenocarcinoma.

Stathmin1 is a microtubular regulatory protein. The expression disorders of this protein result in significant changes in cell migration, invasion, adhesion and colony formation in many malignant tumors. The aim of our research was to investigate the effects of Stathmin1 expression on neoangiogenesis in colorectal adenocarcinoma. Biopsy material that was obtained by the resection of colorectal carcinoma was used. The experimental group consisted of operative biopsies of colorectal cancer (n = 72), and the control group (n = 72) consisted of biopsies of adjacent non-tumor colon tissue. The biopsy material was taken from an operative preparation submitted to the Department of Pathology. After histopathological treatment, classical Hematoxylin- Eosin and immunohistochemical ABC methods with anti-Stathmin1, anti-VEGF and anti CD105 antibodies were applied on 4 µm thick sections. High expression of Stathmin1 is associated with severe (91.9%) and moderate (8.1%) expression of VEGF in a significantly high number of cases. This relation is defined by a highly significant correlation coefficient (r = 0.768; p = 0.000). High expression of Stathmin1 is associated with a high microvascular density index (mvdIDX) in a significant number of cases (73.0%) while low expression of Stathmin1 is in relation with low mvdIDX in a significant 73.7% of cases. This relationship is also defined by a highly significant correlation coefficient (r = 0.566; p = 0.000). ROC analysis showed that the sensitivity for Stathmin1 was 97.4% and the specificity was 91.4%. Based on Stathmin1 expression, it is possible to differentiate patients with increased risk for metastatic disease. The highly significant association of Stathmin1 expression with VEGF expression and microvascular density (MVD) suggests that Stathmin1 may be a serious candidate for therapeutic target.

Clinical and pathological significance of proliferation index and p53 expression in gastric adenocarcinoma.

PURPOSE: The purpose of our work was to investigate the association between proliferative index [proIDX] and expression index p53 (p53IDX) with the clinical and pathological characteristics of gastric adenocarcinoma. METHODS: The biopsy material of 90 patients operated on for gastric cancer was routinely processed in paraffin and archived. After the histopathological report was made, two study groups were formed, the first group (n=45) comprised biopsies with intestinal carcinoma and the second (n=45) biopsies of diffuse gastric cancer. In both cases, the control group consisted of biopsies of surrounding non-tumor tissue The routine Hematoxylin-Eosin and immunohistochemical ABC method with anti-Ki67 and anti-p53 antibodies was applied at sections 3-5 µm thick. The expression of Ki67 and p53 was quantified stereometrically. For statistical analysis SPSS (19.0) was used. RESULTS: Significantly higher Ki67 expression was found in both types of adenocarcinoma compared to the control group, as well as significant association of proIDX with most of testing parameters. Expression of p53 was significantly higher in the intestinal type compared to the diffuse type and the control group and was significantly associated with age and histological grade. Diffuse type particulary showed, significant association of p53IDX with most of the histological parameters tested. CONCLUSION: Our results point a highly significant correlation of the Ki67 and p53 expression with indicators of gastric adenocarcinoma progression, which may help to identify patients with an aggressive gastric adenocarcinoma phenotype.

Clinical significance of Leptin receptor (LEPR) and Endoglin (CD105) expressions in colorectal adenocarcinoma.

PURPOSE: Carcinoma of the colon occurs quite more often in obese than in healthy people. The key molecule in the development of obesity is leptin, a product of Ob gene that expresses its effects through a specific receptor (LEPR), so our goal was to investigate the expression of LEPR in colorectal carcinoma and the association of their expression with neoangiogenesis, with local/regional and distant metastases and with tumor stage according to the Astler-Coller classification. METHODS: In the paraffin blocks taken from 75 patients treated for colorectal cancer, 3-4 µm thick cuts were made using routine hematoxylin-eosin (HE) and immunohistochemical ABC methods with anti-LEPR and anti-CD105 antibodies. After quantitative analysis of LEPR expression, the microvascular density per mm2 was calculated stereometrically. For the statistical processing, the SPSS software (version 13.0) was used. RESULTS: Pronounced expression of LEPR in stages B1 and B2 was present in 9.1% and in 16% of the cases. In the C2 and D stages, pronounced LEPR expression was found in 51.6%, i.e. 57.1% of the cases, which was significantly higher than in the stages B1 and B2. In the C2 and D stages, a high neoangiogenesis index was found in a significantly higher number of cases (67.7% and 100%) than in stages B1 and B2. LEPR expression had a highly significant correlation coefficient associated with tumor stage, neoangiogenesis index, metastases in the lymph nodes and with distant metastases. CONCLUSION: The increase of LEPR expression was accompanied by increased neoangiogenesis and an increase in the metastatic potential of colorectal cancer.

Interplay between expression of leptin receptors and mucin histochemical aberrations in colorectal adenocarcinoma.

BACKGROUND: There is no information on the effects of leptin receptors expression on mucin-histochemical alterations in human colorectal adenocarcinoma. AIM: Testing the correlation of leptin receptors expression with histochemical dysregulation of mucins in colorectal adenocarcinoma. PATIENTS AND METHODS: The study included 75 patients with colorectal adenocarcinoma who underwent surgical resection. Following a routine histopathological tissue analysis, 3-4 µm thick cuts were made onto resected tumors, which underwent a routine Hematoxylin-Eosin, histochemical Alcian Blue-Periodic Acid Schiff, pH 2.5, and High Iron Diamine-Alcian Blue, pH 2.5, methods for mucin differentiation and immunohistochemical Avidin-Biotin peroxidase complex method with anti-Ki67 and anti-leptin receptor antibodies. Following the quantification of results for the statistical analysis, the statistical software package SPSS for Windows (13.0) was used, and the tests for analyzing the significance of differences and correlation analysis - Spearman's rank correlation coefficient, were conducted. RESULTS: Increased expression of leptin receptors is with highly significant correlation coefficient associated with hypersecretion of sialomucins. Significant positive correlation coefficient exists between the leptin receptors expression against neutral-fucomucins secretion. With weak and negative, but a significant correlation coefficient, leptin receptors expression is associated to the sulfomucins generation. CONCLUSIONS: Increased expression of leptin receptors in colorectal adenocarcinoma is associated with mucin-histochemical abnormalities that are manifested by sialomucins hypersecretion and reduction, ultimately resulting in the absence of sulfomucins secretion.

Involvement of leptin receptors expression in proliferation and neoangiogenesis in colorectal carcinoma.

PURPOSE: This study tested whether there exists a correlation between leptin receptors (LEPR) expression with proliferation and neoangiogenesis in colorectal carcinoma. METHODS: Enrolled were 75 patients with colorectal carcinoma, who underwent surgical tumor resection. After routine histopathological preparation, sections 3-4 µm thick were prepared. Routine H&E and immunohistochemical ABC method with anti-LEPR, anti-Ki67 and anti-CD 105 antibodies were performed. RESULTS: Pronounced or moderate LEPR expression in colorectal carcinoma was found in 77.3% of the cases. Absence of expression of LEPR correlated with low rate of proliferation in 94.1% of the cases, while high proliferation rate showed 92% of the cases with pronounced LEPR expression. Low grade neoangiogenesis correlated with absence of LEPR expression in 88.2% of the cases. In 92% of the cases with pronounced LEPR expression, high rate of angiogenesis was observed. The LEPR expression correlated significantly (p<0.001) with proliferation index (proIDX) and neoangiogenesis index (mvdIDX). The corresponded correlation coefficients indicated considerable strength of association between variables (r=0.63 and r=0.66). CONCLUSION: Our results demonstrated that LEPR expression in colorectal carcinoma significantly corresponded to proliferation index of tumor cells and neoangiogenesis, which could have significant therapeutic and prognostic implications.