[The use of cyclophosphane in the immunodepressive therapy of severe aplastic anemia]. / Ispol'zovanie tsiklofosfana v immunodepressivnoi terapii tiazheloi aplasticheskoi anemii.

Low course doses of cyclophosphamide (1.9 +/- 0.2 g) were given to postsplenectomy patients with grave aplastic anemia (GAA). A total of 27 patients aged 14-42 years were treated, 27 control GAA patients did not receive cyclophosphamide. The cyclophosphamide therapy was controlled by T-lymphocyte functional activity assessed in active rosette formation test with levamisole in vitro. 19 (70.3%) patients were treated twice. The remission was achieved in 22 patients (81.5%), partial response was in 1 patient (3.7%), 4 patients died (14.8%). In control subjects the remission was recorded only in 4 subjects, 23 patients died 3 to 12 months after splenectomy. It is evident that low-dose courses of cyclophosphamide are highly effective in case of control over immunological activity of the pathological process.

[Biochemical and cytochemical criteria for predicting the course of acute lymphoblastic leukemia in children]. / Biokhimicheskie i tsitokhimicheskie kriterii prognozirovaniia techeniia ostrogo limfoblastnogo leikoza u detei.

Cytochemical parameters of intracellular metabolism of leukemia cells and bone marrow polyamines were studied in 113 acute lymphoblastic leukemia patients aged from 1 to 14 years. The parameters analyzed characterized the severity of the disease course, cytostatic therapy effectiveness and primary resistance. It is shown that determination of biochemical and cytochemical criteria before the beginning of acute lymphoblastic leukemia therapy may be conducive to the correct choice of treatment.

[Significance of polyamines for the early diagnosis of spontaneous hemoblastoses in AKR mice]. / Znachenie poliaminov dlia rannei diagnostiki spontannykh gemoblastozov myshei AKR.

Polyamines levels were studied in various tissue specimens obtained from high-leukemia AKR mice aged 12 months and less. Organ tissues of leukemic animals revealed increased levels of polyamines and changes in their profile. In symptomatic mice, this was matched by relevant morphologic changes. It is inferred that putrescine is a marker of latent leukemia development.