Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 14 de 14
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
2.
Trends Cogn Sci ; 25(4): 265-268, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33608214

RESUMO

Legacy conferences are costly and time consuming, and exclude scientists lacking various resources or abilities. During the 2020 pandemic, we created an online conference platform, Neuromatch Conferences (NMC), aimed at developing technological and cultural changes to make conferences more democratic, scalable, and accessible. We discuss the lessons we learned.


Assuntos
Pandemias , Humanos
3.
PLoS Comput Biol ; 14(5): e1006157, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29782491

RESUMO

In recent years, two-photon calcium imaging has become a standard tool to probe the function of neural circuits and to study computations in neuronal populations. However, the acquired signal is only an indirect measurement of neural activity due to the comparatively slow dynamics of fluorescent calcium indicators. Different algorithms for estimating spike rates from noisy calcium measurements have been proposed in the past, but it is an open question how far performance can be improved. Here, we report the results of the spikefinder challenge, launched to catalyze the development of new spike rate inference algorithms through crowd-sourcing. We present ten of the submitted algorithms which show improved performance compared to previously evaluated methods. Interestingly, the top-performing algorithms are based on a wide range of principles from deep neural networks to generative models, yet provide highly correlated estimates of the neural activity. The competition shows that benchmark challenges can drive algorithmic developments in neuroscience.


Assuntos
Potenciais de Ação/fisiologia , Cálcio/metabolismo , Biologia Computacional/métodos , Modelos Neurológicos , Algoritmos , Animais , Cálcio/química , Cálcio/fisiologia , Bases de Dados Factuais , Camundongos , Imagem Molecular , Imagem Óptica , Retina/citologia , Neurônios Retinianos/citologia , Neurônios Retinianos/metabolismo
4.
Surv Ophthalmol ; 63(5): 700-710, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-28887138

RESUMO

Cataract extraction is a safe and effective surgery that has been performed in its modern form for several decades. Many studies have noted that cataract extraction could also have a clinically significant role in the control of comorbid glaucoma. Lens extraction decreases the pressure within the eye, and intraocular pressure (IOP) is the only controllable risk factor in glaucoma proven to be effective. A systematic analysis of current evidence is needed to establish strong practice patterns and identify areas where further research is required. We performed systematic review and meta-analysis of the clinical data to estimate the net effect of cataract surgery on IOP. A total of 37 treatment arms from 32 different studies from January 1997 to January 2017 were included. IOP reduction was highly correlated across follow-up periods. For angle-closure glaucoma, results showed an IOP decrease of -6.4 mmHg (95% CI: -9.4 to -3.4) at final follow-up (12 months and longer). For the open-angle glaucoma group, there was an overall IOP change of -2.7 mmHg (95% CI -3.7 to -1.7) from baseline. For pseudoexfoliation glaucoma further research is needed to reach an adequate evidence-based conclusion. The influence of inherent sources of bias, including loss to follow-up, washout and medication use, and lack of a control group, was evaluated numerically. These sources of bias pulled the IOP estimate in opposite directions and are therefore unlikely to affect the main conclusions substantially. Future prospective clinical trials, including other outcomes such as quality of life, clinical severity information, and cost-effectiveness analysis, are needed to determine the role of phacoemulsification alone within the glaucoma treatment algorithm.


Assuntos
Glaucoma/cirurgia , Hipertensão Ocular/cirurgia , Facoemulsificação/métodos , Anti-Hipertensivos/uso terapêutico , Glaucoma/fisiopatologia , Humanos , Pressão Intraocular/fisiologia
5.
J Neurosci ; 36(35): 9227-39, 2016 08 31.
Artigo em Inglês | MEDLINE | ID: mdl-27581462

RESUMO

UNLABELLED: Psychophysical studies have shown that subjects are often unaware of visual stimuli presented around the time of an eye movement. This saccadic suppression is thought to be a mechanism for maintaining perceptual stability. The brain might accomplish saccadic suppression by reducing the gain of visual responses to specific stimuli or by simply suppressing firing uniformly for all stimuli. Moreover, the suppression might be identical across the visual field or concentrated at specific points. To evaluate these possibilities, we recorded from individual neurons in cortical area V4 of nonhuman primates trained to execute saccadic eye movements. We found that both modes of suppression were evident in the visual responses of these neurons and that the two modes showed different spatial and temporal profiles: while gain changes started earlier and were more widely distributed across visual space, nonspecific suppression was found more often in the peripheral visual field, after the completion of the saccade. Peripheral suppression was also associated with increased noise correlations and stronger local field potential oscillations in the α frequency band. This pattern of results suggests that saccadic suppression shares some of the circuitry responsible for allocating voluntary attention. SIGNIFICANCE STATEMENT: We explore our surroundings by looking at things, but each eye movement that we make causes an abrupt shift of the visual input. Why doesn't the world look like a film recorded on a shaky camera? The answer in part is a brain mechanism called saccadic suppression, which reduces the responses of visual neurons around the time of each eye movement. Here we reveal several new properties of the underlying mechanisms. First, the suppression operates differently in the central and peripheral visual fields. Second, it appears to be controlled by oscillations in the local field potentials at frequencies traditionally associated with attention. These results suggest that saccadic suppression shares the brain circuits responsible for actively ignoring irrelevant stimuli.


Assuntos
Neurônios/fisiologia , Movimentos Sacádicos/fisiologia , Córtex Visual/citologia , Córtex Visual/fisiologia , Campos Visuais/fisiologia , Percepção Visual/fisiologia , Potenciais de Ação/fisiologia , Animais , Atenção/fisiologia , Potenciais Evocados Visuais/fisiologia , Feminino , Análise de Fourier , Macaca mulatta , Masculino , Estimulação Luminosa , Tempo de Reação/fisiologia , Estatísticas não Paramétricas
6.
Nat Commun ; 7: 12270, 2016 08 02.
Artigo em Inglês | MEDLINE | ID: mdl-27481398

RESUMO

The primary visual cortex of higher mammals is organized into two-dimensional maps, where the preference of cells for stimulus parameters is arranged regularly on the cortical surface. In contrast, the preference of neurons in the rodent appears to be arranged randomly, in what is termed a salt-and-pepper map. Here we revisited the spatial organization of receptive fields in mouse primary visual cortex by measuring the tuning of pyramidal neurons in the joint orientation and spatial frequency domain. We found that the similarity of tuning decreases as a function of cortical distance, revealing a weak but statistically significant spatial clustering. Clustering was also observed across different cortical depths, consistent with a columnar organization. Thus, the mouse visual cortex is not strictly a salt-and-pepper map. At least on a local scale, it resembles a degraded version of the organization seen in higher mammals, hinting at a possible common origin.


Assuntos
Orientação/fisiologia , Células Piramidais/fisiologia , Córtex Visual/fisiologia , Vias Visuais/fisiologia , Percepção Visual/fisiologia , Animais , Feminino , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Modelos Animais , Modelos Neurológicos , Estimulação Luminosa , Córtex Visual/citologia , Campos Visuais/fisiologia
7.
J Neurosci ; 36(24): 6382-92, 2016 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-27307228

RESUMO

UNLABELLED: We do not fully understand how behavioral state modulates the processing and transmission of sensory signals. Here, we studied the cortical representation of the retinal image in mice that spontaneously switched between a state of rest and a constricted pupil, and one of active locomotion and a dilated pupil, indicative of heightened attention. We measured the selectivity of neurons in primary visual cortex for orientation and spatial frequency, as well as their response gain, in these two behavioral states. Consistent with prior studies, we found that preferred orientation and spatial frequency remained invariant across states, whereas response gain increased during locomotion relative to rest. Surprisingly, relative gain, defined as the ratio between the gain during locomotion and the gain during rest, was not uniform across the population. Cells tuned to high spatial frequencies showed larger relative gain compared with those tuned to lower spatial frequencies. The preferential enhancement of high-spatial-frequency information was also reflected in our ability to decode the stimulus from population activity. Finally, we show that changes in gain originate from shifts in the operating point of neurons along a spiking nonlinearity as a function of behavioral state. Differences in the relative gain experienced by neurons with high and low spatial frequencies are due to corresponding differences in how these cells shift their operating points between behavioral states. SIGNIFICANCE STATEMENT: How behavioral state modulates the processing and transmission of sensory signals remains poorly understood. Here, we show that the mean firing rate and neuronal gain increase during locomotion as a result in a shift of the operating point of neurons. We define relative gain as the ratio between the gain of neurons during locomotion and rest. Interestingly, relative gain is higher in cells with preferences for higher spatial frequencies than those with low-spatial-frequency selectivity. This means that, during a state of locomotion and heightened attention, the population activity in primary visual cortex can support better spatial acuity, a phenomenon that parallels the improved spatial resolution observed in human subjects during the allocation of spatial attention.


Assuntos
Atenção/fisiologia , Locomoção/fisiologia , Neurônios/fisiologia , Orientação Espacial/fisiologia , Córtex Visual/citologia , Córtex Visual/fisiologia , Potenciais de Ação/fisiologia , Animais , Feminino , Modelos Lineares , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Estimulação Luminosa , Transdução Genética
8.
Neuron ; 85(3): 615-27, 2015 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-25600124

RESUMO

Traveling waves of neural activity are frequently observed to occur in concert with the presentation of a sensory stimulus or the execution of a movement. Although such waves have been studied for decades, little is known about their function. Here we show that traveling waves in the primate extrastriate visual cortex provide a means of integrating sensory and motor signals. Specifically, we describe a traveling wave of local field potential (LFP) activity in cortical area V4 of macaque monkeys that is triggered by the execution of saccadic eye movements. These waves sweep across the V4 retinotopic map, following a consistent path from the foveal to the peripheral representations of space; their amplitudes correlate with the direction and size of each saccade. Moreover, these waves are associated with a reorganization of the postsaccadic neuronal firing patterns, which follow a similar retinotopic progression, potentially prioritizing the processing of behaviorally relevant stimuli.


Assuntos
Desempenho Psicomotor/fisiologia , Tempo de Reação/fisiologia , Movimentos Sacádicos/fisiologia , Córtex Visual/fisiologia , Percepção Visual/fisiologia , Animais , Macaca mulatta , Estimulação Luminosa/métodos , Distribuição Aleatória
9.
Neuron ; 79(5): 833-5, 2013 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-24011999

RESUMO

Visual objects tend to be found in predictable combinations (e.g., pens with paper). How does the brain represent these regularities? In this issue of Neuron, Stansbury et al. (2013) use fMRI to study the brain's representation of visual scene categories.


Assuntos
Aprendizagem/fisiologia , Reconhecimento Visual de Modelos/fisiologia , Córtex Visual/fisiologia , Humanos
10.
Artigo em Inglês | MEDLINE | ID: mdl-23533106

RESUMO

Local field potentials (LFP) reflect the properties of neuronal circuits or columns recorded in a volume around a microelectrode (Buzsáki et al., 2012). The extent of this integration volume has been a subject of some debate, with estimates ranging from a few hundred microns (Katzner et al., 2009; Xing et al., 2009) to several millimeters (Kreiman et al., 2006). We estimated receptive fields (RFs) of multi-unit activity (MUA) and LFPs at an intermediate level of visual processing, in area V4 of two macaques. The spatial structure of LFP receptive fields varied greatly as a function of time lag following stimulus onset, with the retinotopy of LFPs matching that of MUAs at a restricted set of time lags. A model-based analysis of the LFPs allowed us to recover two distinct stimulus-triggered components: an MUA-like retinotopic component that originated in a small volume around the microelectrodes (~350 µm), and a second component that was shared across the entire V4 region; this second component had tuning properties unrelated to those of the MUAs. Our results suggest that the LFP reflects neural activity across multiple spatial scales, which both complicates its interpretation and offers new opportunities for investigating the large-scale structure of network processing.

11.
Proc Natl Acad Sci U S A ; 109(16): E972-80, 2012 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-22308392

RESUMO

Neurons in the medial superior temporal (MST) area of the primate visual cortex respond selectively to complex motion patterns defined by expansion, rotation, and deformation. Consequently they are often hypothesized to be involved in important behavioral functions, such as encoding the velocities of moving objects and surfaces relative to the observer. However, the computations underlying such selectivity are unknown. In this work we have developed a unique, naturalistic motion stimulus and used it to probe the complex selectivity of MST neurons. The resulting data were then used to estimate the properties of the feed-forward inputs to each neuron. This analysis yielded models that successfully accounted for much of the observed stimulus selectivity, provided that the inputs were combined via a nonlinear integration mechanism that approximates a multiplicative interaction among MST inputs. In simulations we found that this type of integration has the functional role of improving estimates of the 3D velocity of moving objects. As this computation is of general utility for detecting complex stimulus features, we suggest that it may represent a fundamental aspect of hierarchical sensory processing.


Assuntos
Macaca mulatta/fisiologia , Percepção de Movimento/fisiologia , Lobo Temporal/fisiologia , Vias Visuais/fisiologia , Potenciais de Ação/fisiologia , Algoritmos , Animais , Modelos Neurológicos , Neurônios/fisiologia , Estimulação Luminosa , Lobo Temporal/citologia , Córtex Visual/citologia , Córtex Visual/fisiologia
12.
J Neurophysiol ; 105(1): 474-86, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21068271

RESUMO

Single neurons carry out important sensory and motor functions related to the larger networks in which they are embedded. Understanding the relationships between single-neuron spiking and network activity is therefore of great importance and the latter can be readily estimated from low-frequency brain signals known as local field potentials (LFPs). In this work we examine a number of issues related to the estimation of spike and LFP signals. We show that spike trains and individual spikes contain power at the frequencies that are typically thought to be exclusively related to LFPs, such that simple frequency-domain filtering cannot be effectively used to separate the two signals. Ground-truth simulations indicate that the commonly used method of estimating the LFP signal by low-pass filtering the raw voltage signal leads to artifactual correlations between spikes and LFPs and that these correlations exert a powerful influence on popular metrics of spike-LFP synchronization. Similar artifactual results were seen in data obtained from electrophysiological recordings in macaque visual cortex, when low-pass filtering was used to estimate LFP signals. In contrast LFP tuning curves in response to sensory stimuli do not appear to be affected by spike contamination, either in simulations or in real data. To address the issue of spike contamination, we devised a novel Bayesian spike removal algorithm and confirmed its effectiveness in simulations and by applying it to the electrophysiological data. The algorithm, based on a rigorous mathematical framework, outperforms other methods of spike removal on most metrics of spike-LFP correlations. Following application of this spike removal algorithm, many of our electrophysiological recordings continued to exhibit spike-LFP correlations, confirming previous reports that such relationships are a genuine aspect of neuronal activity. Overall, these results show that careful preprocessing is necessary to remove spikes from LFP signals, but that when effective spike removal is used, spike-LFP correlations can potentially yield novel insights about brain function.


Assuntos
Potenciais de Ação/fisiologia , Algoritmos , Teorema de Bayes , Potenciais Evocados Visuais/fisiologia , Córtex Visual/fisiologia , Animais , Fenômenos Eletrofisiológicos , Macaca mulatta , Modelos Animais , Rede Nervosa/fisiologia
13.
Artigo em Inglês | MEDLINE | ID: mdl-22255055

RESUMO

Surround suppression is a common feature of sensory neurons. For neurons of the visual cortex, it occurs when a visual stimulus extends beyond a neuron's classical receptive field, reducing the neuron's firing rate. While several studies have been attributing the suppression effect on horizontal, long-range lateral or feedback connections, the underlying circuitry for surround modulation remain unidentified. Since most of these models have been relying on single neuron recordings, the contribution of lateral connections can only be suggested from the surround field properties. A more straightforward approach would be to detect these connections and their dynamics using simultaneous recordings from multiple neurons in one or more visual areas. We have developed a method for estimating these connections and we analyzed data obtained from 100-electrode Utah arrays chronically implanted into area V4 of the macaque monkey. Using a method based on the nonlinear Volterra modeling approach, we computed estimates of the strength and statistical reliability of connections among neurons, including nonlinear interactions and excitatory and inhibitory connections. Our results thus far reveal a pattern of connectivity within V4 that conforms to the results of previous anatomical work: Excitatory connections are far more common than inhibitory connections (∼65%), stronger connections are found among neurons that are physically near one another, and connections are stronger among neurons with similar receptive field properties. However, this connectivity is capable of reorganizing on short time scales according to the stimulus: Stimuli that evoke strong suppression at the single-unit level introduce stronger inhibition among V4 neurons, identifying recurrent connectivity as the source of the suppression. Overall, these results provide insight into the dynamic nature of neuronal organization within V4 and its contribution to surround suppression.


Assuntos
Córtex Visual/fisiologia , Animais , Macaca mulatta , Neurônios/fisiologia , Córtex Visual/citologia
14.
J Vis ; 9(10): 17.1-24, 2009 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-19810798

RESUMO

Classification images provide compelling insight into the strategies used by observers in psychophysical tasks. However, because of the high-dimensional nature of classification images and the limited quantity of trials that can practically be performed, classification images are often too noisy to be useful unless denoising strategies are adopted. Here we propose a method of estimating classification images by the use of sparse priors in smooth bases and generalized linear models (GLMs). Sparse priors in a smooth basis are used to impose assumptions about the simplicity of observers' internal templates, and they naturally generalize commonly used methods such as smoothing and thresholding. The use of GLMs in this context provides a number of advantages over classic estimation techniques, including the possibility of using stimuli with non-Gaussian statistics, such as natural textures. Using simulations, we show that our method recovers classification images that are typically less noisy and more accurate for a smaller number of trials than previously published techniques. Finally, we have verified the efficiency and accuracy of our approach with psychophysical data from a human observer.


Assuntos
Modelos Lineares , Modelos Psicológicos , Psicofísica/métodos , Percepção Visual/fisiologia , Adulto , Classificação , Simulação por Computador , Humanos , Funções Verossimilhança , Masculino , Distribuição Normal
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...