RESUMO
Methanolic extract and two purified compounds (brazilin and hematoxylin) from Caesalpinia sappan were examined for their relaxant effects in isolated rat thoracic aorta. The methanolic extract significantly and dose-dependently relaxed the alpha1-receptor agonist phenylephrine-precontracted aortic rings, without affecting passive tension of these vessels. Removal of the vascular endothelium, inhibition of nitric oxide (NO) synthase with 0.1 mM Nomega-nitro-L-arginine and of cGMP biosynthesis with 10 microM methylene blue abolished the vasorelaxant effects of the herbal extract at doses up to 30 microg/ml. Similar vasorelaxant effects were observed with brazilin and hematoxylin. Therefore, these results suggest that brazilin and hematoxylin may be responsible for the vascular relaxant effects of C. sappan, via endogenous NO and subsequent cGMP formation. The vascular relaxant effects of the plant may contribute to its therapeutic actions.
Assuntos
Aorta Torácica/enzimologia , Medicamentos de Ervas Chinesas , Músculo Liso Vascular/enzimologia , Óxido Nítrico Sintase/metabolismo , Vasodilatadores , Animais , Aorta Torácica/efeitos dos fármacos , Benzopiranos/farmacologia , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Endotélio Vascular/efeitos dos fármacos , Endotélio Vascular/enzimologia , Fabaceae/química , Fabaceae/uso terapêutico , Hematoxilina/farmacologia , Técnicas In Vitro , Masculino , Contração Muscular/efeitos dos fármacos , Músculo Liso Vascular/efeitos dos fármacos , Óxido Nítrico Sintase Tipo III , Fitoterapia , Plantas Medicinais , Ratos , Ratos Sprague-Dawley , Vasodilatadores/química , Vasodilatadores/isolamento & purificação , Vasodilatadores/farmacologiaRESUMO
Geumonoid (1), a new triterpene, was isolated from Geum japonicum. Its structure was elucidated on the basis of 1D, 2D NMR and MS spectroscopic analysis. Compound 1 showed inhibitory activity against HIV-1 protease.
Assuntos
Fármacos Anti-HIV/isolamento & purificação , Inibidores da Protease de HIV/síntese química , HIV-1/enzimologia , Triterpenos/isolamento & purificação , Fármacos Anti-HIV/farmacologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/enzimologia , Inibidores da Protease de HIV/farmacologia , HIV-1/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Triterpenos/farmacologiaRESUMO
AIM: To investigate the chemical constituents of Murraya kwangsiensis. METHODS: Compounds were isolated by silica gel column chromatography and TLC method, respectively. Structures of compounds were elucidated by spectral (UV, IR, MS, 1HNMR and 13CNMR) analysis. RESULTS: Five carbazole alkaloids and two other compounds were isolated and identified. Six of them are known compounds: murrayafoline A, murrayanine, koenine, isomahanine, palmitic acid and beta-sitosterol. One is a new alkaloid, named kwangsine. CONCLUSION: A new alkaloid, named kwangsine, was isolated from Murraya kwangsiensis Var. Six known compounds were isolated from this plant for the first time.
Assuntos
Alcaloides/química , Plantas Medicinais/química , Alcaloides/isolamento & purificaçãoRESUMO
Two new caffeoyl glycosides of phenethyl alcohol, suspensaside A (1) and suspensaside B (2), were isolated from the fruits of Forsythia suspensa. Also obtained in this investigation were two known compounds forsythiaside (3) and suspensaside (4). The structures of compounds 1 and 2 were established by ID and 2D NMR techniques and chemical methods.
Assuntos
Ácidos Cafeicos/isolamento & purificação , Glicosídeos/isolamento & purificação , Magnoliopsida/química , Ácidos Cafeicos/química , Glucosídeos/química , Glicosídeos/química , Espectroscopia de Ressonância Magnética , Modelos QuímicosRESUMO
A new furofuran mono-lactone, named forsythenin, was isolated from the fruits of Forsythia suspensa, together with the known compounds, ocotillone, ocotillol monoacetate, (6'-O-palmitoyl)-sitosterol-3-O-beta-D-glucoside and palmitic acid. The structure of the new compound was elucidated on the basis of spectroscopic means and X-ray crystallography.
