RESUMO
[Objective] To observe the intervention effect on body conditioning cream on the constitution of qi deficiency, Yang deficiency constitution. [Methods] Of the 200 patients with Qi deficiency, Yang deficiency condition of outpatients were randomly divided into experimental group and control group with 100 cases in each. In the experimental group, the control group took general body-distinguished mutrition intervention ,every year, starting from 10d before the winter solstice to the spring after taking 10d ,about taking 60d for a course of treatment. 1 year after the end of the treatment, evaluate the efficacy and safety.[Result] In the experimental group after the intervention, Qi deficiency and yang deficiency constitution overal clinical scores were better than before intervention,and the experimental group intervention was superior to the control group. [Conclusion] The Body-distinguished Cream intervention has strong interaction on Qi deficiency, Yang deficiency.
RESUMO
In this paper, two recombinant plasmids (pVIR-P12AIL18-3C and pVIR-P12A-3C) containing foot and mouth disease virus (FMDV) capsid polypeptide, 3C coding regions of O/NY00 and using/or not swine IL18 as a genetic adjuvant were constructed, and evaluated for their ability to induce humoral and cellular responses in mice and swine. In addition, the ability to protect swine against homologous virus challenge was examined. Mice and swine were given booster vaccination twice and once, respectively, and swine were challenged 10 days after the booster vaccination. Control groups were inoculated with pVAX1 or phosphate-buffered saline (PBS). All animals vaccinated with pVIR-P12AIL18-3C and pVIR-P12A-3C developed specific anti-FMDV ELISA antibody and neutralizing antibody and T lymphocyte proliferation and CTL cytotoxic activity was observed. In addition, we found that pVIR-P12AIL18-3C possessed stronger immunogenicity than pVIR-P12A-3C. The pVIR-P12AIL18-3C and pVIR-P12A-3C provided full protection in 3/4 and 2/4 swine from challenge with FMDV O/NY00, respectively. The results demonstrate the potential viability of a DNA vaccine in the control and prevention of FMDV infections.
