Conformational dynamics in the disordered region of human CPEB3 linked to memory consolidation.

BACKGROUND: Current understanding of the molecular basis of memory consolidation points to an important function of amyloid formation by neuronal-specific isoforms of the cytoplasmic polyadenylation element binding (CPEB) protein family. In particular, CPEB is thought to promote memory persistence through formation of self-sustaining prion-like amyloid assemblies at synapses, mediated by its intrinsically disordered region (IDR) and leading to permanent physical alterations at the basis of memory persistence. Although the molecular mechanisms by which amyloid formation takes place in CPEB have been described in invertebrates, the way amyloid formation occurs in the human homolog CPEB3 (hCPEB3) remains unclear. Here, we characterize by NMR spectroscopy the atomic level conformation and ps-ms dynamics of the 426-residue IDR of hCPEB3, which has been associated with episodic memory in humans. RESULTS: We show that the 426-residue N-terminal region of hCPEB3 is a dynamic, intrinsically disordered region (IDR) which lacks stable folded structures. The first 29 residues, M1QDDLLMDKSKTQPQPQQQQRQQQQPQP29, adopt a helical + disordered motif, and residues 86-93: P83QQPPPP93, and 166-175: P166PPPAPAPQP175 form polyproline II (PPII) helices. The (VG)5 repeat motif is completely disordered, and residues 200-250 adopt three partially populated α-helices. Residues 345-355, which comprise the nuclear localization signal (NLS), form a modestly populated α-helix which may mediate STAT5B binding. These findings allow us to suggest a model for nascent hCPEB3 structural transitions at single residue resolution, advancing that amyloid breaker residues, like proline, are a key difference between functional versus pathological amyloids. CONCLUSION: Our NMR spectroscopic analysis of hCPEB3 provides insights into the first structural transitions involved in protein-protein and protein-mRNA interactions. The atomic level understanding of these structural transitions involved in hCPEB3 aggregation is a key first step toward understanding memory persistence in humans, as well as sequence features that differentiate beneficial amyloids from pathological ones. AREAS: Biophysics, Structural Biology, Biochemistry & Neurosciences.

Results of the short neck-retaining stem prostheses CFP with a minimum follow-up of 10 years. / Resultado del vástago corto con preservación del cuello femoral CFP con un seguimiento mínimo de 10 años.

BACKGROUND AND OBJECTIVE: Bone-preserving implants in primary total hip arthroplasty have become an increasingly popular treatment choice, especially short stems. Currently there are many models with morphological and biodynamic differences, among which stand out the neck-retaining systems, but there are not many studies with a long-term follow-up of these systems. The objective of this study is to retrospectively analyze the results of the Collum Femoris Preserving (CFP) short neck-retaining stem system with a minimum follow-up of 10 years. MATERIAL AND METHODS: Between 2001 and 2010, 175 prostheses with a CFP stem were performed in 157 patients in our hospital. All patients were operated on by a single surgeon. The mean age at the time of surgery was 56.47 years (range 22-77). RESULTS: 175 prostheses have been evaluated with a minimum follow-up of 10 years and a mean of 13.89 years (range 10-19), with 16 cases having been lost. During the follow-up there were 8 polyethylene replacements, 2 cup replacements due to loosening, 2 osteosynthesis due to fracture without loosening, and 5 stem replacements: 1 due to chronic infection, 2 due to fracture and 2 due to aseptic loosening. CONCLUSIONS: As far as we know, this is the first follow-up work on a short stem implanted in our country with a minimum of 10 years of follow-up. The replacement of this stem due to aseptic loosening has been 2/159 and, therefore, survival for this reason is 98.75% at 10 years.

Resultado del vástago corto con preservación del cuello femoral CFP con un seguimiento mínimo de 10 años / Results of the short neck-retaining stem prostheses CFP with a minimum follow-up of 10 years

Antecedentes y objetivo: Las técnicas de preservación ósea en prótesis totales de cadera primarias han experimentado un gran auge en los últimos años, especialmente los vástagos cortos. Actualmente existen muchos modelos con diferencias morfológicas y biodinámicas, entre los cuales destacan los sistemas con preservación del cuello femoral, pero no existen muchos trabajos con un seguimiento a largo plazo de estos sistemas. El objetivo de este trabajo es analizar retrospectivamente el resultado del sistema de vástago corto con preservación de cuello femoral Collum Femoris Preserving (CFP) con un seguimiento mínimo de 10 años. Material y métodos: Entre los años 2001 y 2010 se realizaron 175 prótesis con vástago CFP en 157 pacientes en nuestro hospital. Todos los pacientes fueron intervenidos por un único cirujano. La edad media en el momento de la cirugía fue de 56,47 años (rango 22-77). Resultados: Se han evaluado 175 prótesis con un seguimiento mínimo de 10 años y una media de 13,89 años (rango 10-19), habiéndose perdido 16 casos. Durante el seguimiento ha habido 8 recambios de polietileno, 2 de cótilo por aflojamiento, 2 osteosíntesis por fractura sin aflojamiento y 5 recambios de vástago: uno por infección crónica, 2 por fractura y 2 por aflojamiento aséptico. Conclusiones: Hasta donde sabemos, es el primer trabajo de seguimiento de un vástago corto implantado en nuestro país con un mínimo de 10 años de evolución. Los recambios de este vástago por aflojamiento aséptico han sido 2/159 y, por tanto, la supervivencia por este motivo es del 98,75% a los 10 años.(AU)

Background and objective: Bone-preserving implants in primary total hip arthroplasty have become an increasingly popular treatment choice, especially short stems. Currently there are many models with morphological and biodynamic differences, among which stand out the neck-retaining systems, but there are not many studies with a long-term follow-up of these systems. The objective of this study is to retrospectively analyze the results of the Collum Femoris Preserving (CFP) short neck-retaining stem system with a minimum follow-up of 10 years. Material and methods: Between 2001 and 2010, 175 prostheses with a CFP stem were performed in 157 patients in our hospital. All patients were operated on by a single surgeon. The mean age at the time of surgery was 56.47 years (range 22–77). Results: 175 prostheses have been evaluated with a minimum follow-up of 10 years and a mean of 13.89 years (range 10–19), with 16 cases having been lost. During the follow-up there were 8 polyethylene replacements, 2 cup replacements due to loosening, 2 osteosynthesis due to fracture without loosening, and 5 stem replacements: 1 due to chronic infection, 2 due to fracture and 2 due to aseptic loosening. Conclusions: As far as we know, this is the first follow-up work on a short stem implanted in our country with a minimum of 10 years of follow-up. The replacement of this stem due to aseptic loosening has been 2/159 and, therefore, survival for this reason is 98.75% at 10 years.(AU)

Results of the short neck-retaining stem prostheses CFP with a minimum follow-up of 10 years / Resultado del vástago corto con preservación del cuello femoral CFP con un seguimiento mínimo de 10 años

Background and objective: Bone-preserving implants in primary total hip arthroplasty have become an increasingly popular treatment choice, especially short stems. Currently there are many models with morphological and biodynamic differences, among which stand out the neck-retaining systems, but there are not many studies with a long-term follow-up of these systems. The objective of this study is to retrospectively analyze the results of the Collum Femoris Preserving (CFP) short neck-retaining stem system with a minimum follow-up of 10 years. Material and methods: Between 2001 and 2010, 175 prostheses with a CFP stem were performed in 157 patients in our hospital. All patients were operated on by a single surgeon. The mean age at the time of surgery was 56.47 years (range 22–77). Results: 175 prostheses have been evaluated with a minimum follow-up of 10 years and a mean of 13.89 years (range 10–19), with 16 cases having been lost. During the follow-up there were 8 polyethylene replacements, 2 cup replacements due to loosening, 2 osteosynthesis due to fracture without loosening, and 5 stem replacements: 1 due to chronic infection, 2 due to fracture and 2 due to aseptic loosening. Conclusions: As far as we know, this is the first follow-up work on a short stem implanted in our country with a minimum of 10 years of follow-up. The replacement of this stem due to aseptic loosening has been 2/159 and, therefore, survival for this reason is 98.75% at 10 years.(AU)

Antecedentes y objetivo: Las técnicas de preservación ósea en prótesis totales de cadera primarias han experimentado un gran auge en los últimos años, especialmente los vástagos cortos. Actualmente existen muchos modelos con diferencias morfológicas y biodinámicas, entre los cuales destacan los sistemas con preservación del cuello femoral, pero no existen muchos trabajos con un seguimiento a largo plazo de estos sistemas. El objetivo de este trabajo es analizar retrospectivamente el resultado del sistema de vástago corto con preservación de cuello femoral Collum Femoris Preserving (CFP) con un seguimiento mínimo de 10 años. Material y métodos: Entre los años 2001 y 2010 se realizaron 175 prótesis con vástago CFP en 157 pacientes en nuestro hospital. Todos los pacientes fueron intervenidos por un único cirujano. La edad media en el momento de la cirugía fue de 56,47 años (rango 22-77). Resultados: Se han evaluado 175 prótesis con un seguimiento mínimo de 10 años y una media de 13,89 años (rango 10-19), habiéndose perdido 16 casos. Durante el seguimiento ha habido 8 recambios de polietileno, 2 de cótilo por aflojamiento, 2 osteosíntesis por fractura sin aflojamiento y 5 recambios de vástago: uno por infección crónica, 2 por fractura y 2 por aflojamiento aséptico. Conclusiones: Hasta donde sabemos, es el primer trabajo de seguimiento de un vástago corto implantado en nuestro país con un mínimo de 10 años de evolución. Los recambios de este vástago por aflojamiento aséptico han sido 2/159 y, por tanto, la supervivencia por este motivo es del 98,75% a los 10 años.(AU)

Asociación entre dedos en resorte múltiples, enfermedades sistémicas y síndrome del túnel carpiano: análisis multivariante / Association between multiple trigger fingers, systemic diseases and carpal tunnel syndrome: a multivariate analysis

Introducción: El dedo en resorte (DR) es una patología frecuente de etiología multifactorial. El objetivo de este estudio fue valorar la relación entre la aparición de DR múltiple y patologías sistémicas o musculoesqueléticas en una muestra de pacientes jóvenes en edad laboral. Material y método: Se analizaron retrospectivamente todos los pacientes con DR intervenidos en nuestro hospital entre 2011 y 2015. Se recogió la existencia de DR múltiple o único y patologías como diabetes mellitus (DM), disfuncionalidad tiroidea, síndrome del túnel carpiano (STC), epicondilalgia o enfermedad de DeQuervain. Los resultados estadísticos incluyen un análisis bivariante y una regresión logística multivariante. Resultados: Se incluyeron 279 pacientes, con una edad media de 48,45+/-9,01años. La mano dominante estuvo afectada en 217 casos. Hubo 59 pacientes con DR múltiple, 21 con DM, 55 STC, 16 epicondilalgia y 14 DeQuervain. La prevalencia de STC fue del 19,7%, significativamente superior a la media poblacional (2-4%). No se hallaron diferencias en cuanto a edad media, sexo, hipotiroidismo, epincondilitis o DeQuervain en DR único o múltiple. El análisis bivariante detectó que la proporción de DM (20,3%) y STC (32,2%) en el grupo de DR múltiple fue significativamente mayor que en el grupo de DR único (DM: 4,1%, p=0,007; STC: 16,4%, p<0,01). El DR múltiple también fue más frecuente en mano dominante (93,2%; p<0,01). La regresión logística multivariante confirmó los hallazgos, mostrando asociación estadísticamente significativa entre el grupo de DR múltiple y DM (OR: 4,98, p<0,01), STC (OR: 2,09, p=0,037) y la mano dominante (OR: 3,76, p=0,016). Conclusiones: La DM, el STC y la mano dominante se relacionan de forma independiente con la aparición de DR múltiples en pacientes jóvenes en edad laboral

Introduction: Trigger finger (TF) is a frequent pathology depending on several factors. The objective of this study was to assess the relationship between multiple TF and systemic or musculoskeletal disorders in a sample of young patients. Material and method: A retrospective study was performed of all patients with TF operated in our hospital between 2011 and 2015. Multiple or single TF diagnosis and pathologies such as diabetes mellitus (DM), thyroid dysfunction, carpal tunnel syndrome (CTS), epicondylalgia or DeQuervain's disease were collected. Statistical results included a bivariate analysis and a multiple logistic regression. Results: Two hundred and seventy-nine patients with a mean age of 48.45years were included. The dominant hand was affected in 217 cases. There were 59 patients with multiple TF, 21 DM, 55 STC, 16 epicondylalgia and 14 DeQuervains. Prevalence of CTS was 19.7%, significantly higher than the general population (2%-4%). No statistical differences were found in age, sex, hypothyroidism, epicondylalgia or DeQuervain in the multiple TF group. Bivariate analysis detected that DM and CTS patients in the multiple TF group was significantly higher than in the single TF group (P=.007, P<.01). Multiple TF was also more frequent on the dominant side (P<.01). Multivariate logistic regression confirmed these findings, showing a statistically significant association between the multiple TF group and DM (OR: 4.98, P<.01), STC (OR: 2.09, P=.037) and dominant side (OR: 3.76, P=.016). Conclusions: Diabetes, CTS and dominant side are independently associated with multiple TF in young patients

Comparison between single portal endoscopic and 1-cm open carpal tunnel release.

Given the controversy about the best surgical technique for carpal tunnel release, this study tested the hypothesis that no significant differences would be observed between single-portal endoscopic release and a short, 1-cm incision open release in a large sample of patients. Consecutive patients were assigned to one of the two techniques. Preoperative and postoperative measurements included grip and pinch strength, a visual analog scale for pain and a satisfaction questionnaire. Eighty-seven patients completed the study: 35 with an endoscopic release and 52 with an open release. Both techniques were effective and safe: grip and pinch strength decreased 1 month after surgery in both techniques but improved significantly at 6 and 12 months (P < 0.05) while the complication rate was low. Subjective results were judged to be "excellent or good" by more than 90% of patients in both groups. No significant between-technique differences in outcomes were observed thus, surgical decisions may be based on criteria other than effectiveness. LEVEL OF EVIDENCE: Level IV; Case Series; Treatment Study.

Association between multiple trigger fingers, systemic diseases and carpal tunnel syndrome: A multivariate analysis. / Asociación entre dedos en resorte múltiples, enfermedades sistémicas y síndrome del túnel carpiano: análisis multivariante.

INTRODUCTION: Trigger finger (TF) is a frequent pathology depending on several factors. The objective of this study was to assess the relationship between multiple TF and systemic or musculoskeletal disorders in a sample of young patients. MATERIAL AND METHOD: A retrospective study was performed of all patients with TF operated in our hospital between 2011 and 2015. Multiple or single TF diagnosis and pathologies such as diabetes mellitus (DM), thyroid dysfunction, carpal tunnel syndrome (CTS), epicondylalgia or DeQuervain's disease were collected. Statistical results included a bivariate analysis and a multiple logistic regression. RESULTS: Two hundred and seventy-nine patients with a mean age of 48.45years were included. The dominant hand was affected in 217 cases. There were 59 patients with multiple TF, 21 DM, 55 STC, 16 epicondylalgia and 14 DeQuervains. Prevalence of CTS was 19.7%, significantly higher than the general population (2%-4%). No statistical differences were found in age, sex, hypothyroidism, epicondylalgia or DeQuervain in the multiple TF group. Bivariate analysis detected that DM and CTS patients in the multiple TF group was significantly higher than in the single TF group (P=.007, P<.01). Multiple TF was also more frequent on the dominant side (P<.01). Multivariate logistic regression confirmed these findings, showing a statistically significant association between the multiple TF group and DM (OR: 4.98, P<.01), STC (OR: 2.09, P=.037) and dominant side (OR: 3.76, P=.016). CONCLUSIONS: Diabetes, CTS and dominant side are independently associated with multiple TF in young patients.

Características clínicas y microbiológicas en queratitis infecciosas bacterianas en un hospital de tercer nivel / Clinical features and microbiological in bacterial keratitis in a tertiary referral hospital

Objetivo: Describir las características clínicas, agentes bacterianos y sensibilidad antibiótica de las queratitis bacterianas en el Servicio de Oftalmología del Hospital Universitario Fundación Jiménez Díaz (HUFJD) de Madrid. Materiales y métodos: Estudio observacional, descriptivo y retrospectivo de los registros clínicos e informes de los raspados corneales en pacientes con queratitis bacterianas en el HUFJD realizados entre los años 2009 y 2014. Resultados: Se tuvo una muestra de 160 pacientes. Las bacterias grampositivas fueron las más prevalentes con un 64,3% (n=103). Staphylococcus coagulasa negativo (20,6%), Staphylococcus aureus (19,4%) y Pseudomonas aeruginosa (12,5%) fueron las bacterias más frecuentes. El factor de riesgo más común fue el uso de lentes de contacto, seguido de enfermedad de la superficie ocular y cirugías oculares previas. Los antibióticos a los que las bacterias fueron más comúnmente sensibles fueron: gentamicina (n=114), cotrimoxazol (n=107), vancomicina (n=106) y ciprofloxacina (n=97) y los antibióticos a los que las bacterias fueron más comúnmente resistentes fueron ampicilina (n=59) y eritromicina (n=45). Conclusiones: En el manejo inicial de las queratitis bacterianas se debería tener en cuenta la sensibilidad y resistencia de las bacterias ante los antibióticos. Recomendamos, con base en nuestros hallazgos, el uso de aminoglucósidos, vancomicina y fluoroquinolonas, e interrumpir el uso de eritromicina, que es ampliamente usada actualmente (AU)

Objective: To describe the clinical features, bacterial agents, and antibiotic sensitivity of bacterial keratitis in the Ophthalmology Department at the University Hospital Fundación Jiménez Díaz (HUFJD) in Madrid. Materials and methods: A retrospective observational descriptive study using clinical records and reports of corneal scrapings in patients with bacterial keratitis at the HUFJD conducted between 2009 and 2014. Results: In a sample of 160 patients, gram-positive bacteria were the most prevalent with 64.3% (n=103). Coagulase negative staphylococcus (20.6%), Staphylococcus aureus (19.4%), and Pseudomonas aeruginosa (12.5%) were the most frequent bacteria. The most common risk factor was the use of contact lenses, followed by disease of the ocular surface, and previous ocular surgeries. The antibiotics to which the bacteria were most commonly susceptible were gentamicin (n=114), cotrimoxazole (n=107), vancomycin (n=106), and ciprofloxacin (n=97). The antibiotics to which the bacteria were most commonly resistant were ampicillin (n=59) and erythromycin (n=45). Conclusions: In the initial management of bacterial keratitis, the sensitivity and resistance of bacteria to antibiotics should be taken into account. Based on our findings, the use of aminoglycosides, vancomycin and fluoroquinolones is recommended, and, although widely used today, the discontinuation of erythromycin (AU)

Clinical features and microbiological in bacterial keratitis in a tertiary referral hospital. / Características clínicas y microbiológicas en queratitis infecciosas bacterianas en un hospital de tercer nivel.

OBJECTIVE: To describe the clinical features, bacterial agents, and antibiotic sensitivity of bacterial keratitis in the Ophthalmology Department at the University Hospital Fundación Jiménez Díaz (HUFJD) in Madrid. MATERIALS AND METHODS: A retrospective observational descriptive study using clinical records and reports of corneal scrapings in patients with bacterial keratitis at the HUFJD conducted between 2009 and 2014. RESULTS: In a sample of 160 patients, gram-positive bacteria were the most prevalent with 64.3% (n=103). Coagulase negative staphylococcus (20.6%), Staphylococcus aureus (19.4%), and Pseudomonas aeruginosa (12.5%) were the most frequent bacteria. The most common risk factor was the use of contact lenses, followed by disease of the ocular surface, and previous ocular surgeries. The antibiotics to which the bacteria were most commonly susceptible were gentamicin (n=114), cotrimoxazole (n=107), vancomycin (n=106), and ciprofloxacin (n=97). The antibiotics to which the bacteria were most commonly resistant were ampicillin (n=59) and erythromycin (n=45). CONCLUSIONS: In the initial management of bacterial keratitis, the sensitivity and resistance of bacteria to antibiotics should be taken into account. Based on our findings, the use of aminoglycosides, vancomycin and fluoroquinolones is recommended, and, although widely used today, the discontinuation of erythromycin.

Patterns of porphyrin-excretion in female estrogen-induced porphyria cutanea tarda.

It has been suggested that the urinary profile of porphyrins excreted by female patients with estrogen-induced porphyria cutanea tarda is peculiar in that heptacarboxylic porphyrin equals or exceeds uroporphyrin. The chromatographic pattern of urinary porphyrin excretion was studied in nine females with porphyria cutanea tarda precipitated by estrogens, 129 porphyric males, and nine females, whose porphyria was not hormone-induced. Both female porphyric groups showed absolutely the same urinary chromatographic pattern and looked quite similar to the pattern observed in male patients. None of our female porphyric patients, whether treated with estrogens or not, showed percentage values for the heptacarboxylic porphyrin higher or equal to the uroporphyrin values. Our results do not support the hypothesis that the profile of urinary porphyrin excretion found in estrogen-induced porphyria cutanea tarda is atypical.