Relationships of concentrations of true insulin, proinsulin to coronary heart disease: a preliminary study / 中华内分泌代谢杂志

Objective　To determine serum true insulin (TI) and proinsulin (PI) levels in patients with coronary heart disease (CHD), and to explore the relationships of them to other cardiovascular risk factors. Methods　Highly specific ELISA assays for TI and PI and RIA for immunoreactive insulin (IRI) were used. The fasting serum IRI, TI, PI levels in 30 nondiabetic patients with CHD and 30 healthy, age-matched control subjects were investigated. And the fasting and 2h postprandial plasma glucose, serum lipids and blood pressure were also determined in each individuals. Results　The levels of serum IRI, TI and PI were significantly higher in patients with CHD than those in controls (P＜0.05), while the value of insulin sensitivity index (ISI) was lower in patients with CHD than in controls (P＜0.05). There were significant independent correlations between TI, PI and age, BMI, plasma glucose, serum lipids. Conclusion Nondiabetic patients with CHD have hyperinsulinemia and insulin resistance and there is an independent association between proinsulin and dyslipidemia.

Investigation on interleukin-1 receptor antagonist gene polymorphism in old men with osteoporosis / 中华内分泌代谢杂志

Objective To investigate the association of interleukin 1 receptor antagonist (IL 1Ra) gene polymorphism with osteoporosis in Chinese old men. Methods The allele frequency and the genotype distribution of the second intron of IL 1Ra gene in the groups of senile control (normal bone mineral density, n=65) and senile men with osteoporosis (n=68) were detected by polymerase chain reaction (PCR). Results The frequencyofA 2alleleincontrolgroup was higher than that in osteoporosis group (0.192 vs 0.103, P

Associaton of polymorphism of endothelial nitric oxide synthase gene with essential hypertension and type 2 diabetes mellitus / 中华内分泌代谢杂志

Objective To investigate the association of the polymorphism of endothelial nitric oxide synthase (eNOS) gene 27bp variable number of tandem repeats (VNTR) with essential hypertension (EH) and type 2 diabetes mellitus (DM) in Chinese population. Methods (1) Genotypes were determined by polymerase chain reaction (PCR)-agarose gel electrophoresis. (2) Fasting serum nitric oxide metabolites (NOx) were measured by nitrate reductase. (3) During oral glucose tolerance test (OGTT) serum immunoreactive insulin (IRI) and C peptide (CP) were measured by radioimmunoassay. Results (1) The frequenciesofaalleleinEHandtype2DMgroupwere significantly higher than that in control group (0.109 vs 0.051; 0.129vs 0.051, P