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Objective To explore the impact of heat shock factor 2 (HSF2) on the development of lung cancer by promoting interleukin (IL)-10 expression.Methods 50 lung cancer tissues and adjacent normal tissues selected from 50 patients,the expression level of mRNA and protein of HSF2 and IL-10 were respectively detected by RT-PCR,Western blot and Immunohistochemistry;To interfere with expression of HSF2 in A549 cells by siRNA,the expression level of IL-10 was detected by Western blot.Results Compared with the adjacent normal tissues,HSF2 of 76％ (38 of 50) cases were up-regulated (P＜0.01),IL-10 of 80％ (40 of 50) eases were up-regulated (P＜0.01),protein level consistent with mRNA level.The up-regulation expression of IL-10 in lung cancer tissues and HSF2 positively correlated (R2 =0.921 6).The expression of IL-10 in A549 cells was weakened through interference with HSF2 by siRNA.Conclusion HSF2 may contribute to the development of lung cancer by facilitating the expression of IL-10.
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Objective The study aimed to analyze the clinical data of patients with pulmonary embolism and summarize the clinical characteristics of pulmonary embolism,in order to improve the correct diagnostic rate and the prognosis. Methods Data of 178 patients who were cured in our hospital from January 2011 to December 2014 were analyzed retrospectively,including relative risk factors,clinical behaviors,examination data,misdiagnosis and prognosis. Results Patients with pulmonary embolism had related basic diseases and risk factors. Their clinical symptoms,signs and ordinary detection methods indicated pneumatorexis and cardiovascular injury,but lacked of specificity. The misdiagnosis rate of the first diagnosis was 48.88%,including 22 cases of the misdiagnosis to COPD, 18 cases to pneumonia and 16 cases to coronary heart disease. The misdiagnosis rate of elderly patients was 56.36%, while that of the young and middle-aged patients was 36.80%,the difference was statistically significant,P 0.05). Conclusion Although the related risk factors of pulmonary embolism in patients existed,the misdiagnosed rate of the disease was high and prognosis was poor due to many confusing basic diseases. And the symptoms,signs and ordinary inspection of the patients lacked specificity. CTPA,being utilized to diagnose pulmonary embolism reliably,should be promoted.
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Objective To study the significance of changes in the blood C-reactive protein(CRP)concentration in patients with chronic obstructive pulmonary disease(COPD)at different stages and different classification and further analyze the relationship between the degree of impairment of pulmonary function and CRP concentrations.Methods Fouty-two cases of COPD patients were enrolled,forced expiratory volume in one second(FEV1),the forced expiratory volume in one second/forced vital capacity(FEV1/FVC),a second rate were tested in these patients at admission and discharged from hospital after recovery.Arterial blood gas including PaO2,PaCO2 were analyzed.The CRP level was measured by ELISA from venous blood collected in the early morning.At the same time,10 healthy people were selected as control group.Results The CRP level in COPD group was significantly higher than that of the control group(P ＜ 0.05).It displayed highest in the Ⅳ grade of COPD patients,but there was no statistically significant differences among the levels(P ＞ 0.05).The CRP level in COPD patients at acute exacerbations was significantly higher than that in COPD patients at remission and differences was statistically significant([6.283 ± 3.168]mg/L vs[4.104 ± 1.013]mg/L,P ＜0.05).The changes of serum CRP levels was correlated negatively with FEV1(r =-0.618,P ＜ 0.05),with FEV1/FVC(r =-0.629,P ＜ 0.05)and with PaO2(r =-0.605,P ＜ 0.05).The changes of serum CRP levels was correlated positively with PaCO2(r =0.613,P ＜ 0.05).Conclusion The increase of CRP level may be associated with the damage of lung function and the degree of dyspnea in COPD patients.But CRP as a independent predictor of COPD outcomes and prognosis remains to be further studied.
