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1.
Plant Physiol Biochem ; 100: 12-17, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26773540

RESUMO

As part of an evolution-function analysis, two nucleobase cation symporter 1 (NCS1) from the moss Physcomitrella patens (PpNCS1A and PpNCS1B) are examined--the first such analysis of nucleobase transporters from early land plants. The solute specificity profiles for the moss NCS1 were determined through heterologous expression, growth and radiolabeled uptake experiments in NCS1-deficient Saccharomyces cerevisiae. Both PpNCS1A and 1B, share the same profiles as high affinity transporters of adenine and transport uracil, guanine, 8-azaguanine, 8-azaadenine, cytosine, 5-fluorocytosine, hypoxanthine, and xanthine. Despite sharing the same solute specificity profile, PpNCS1A and PpNCS1B move nucleobase compounds with different efficiencies. The broad nucleobase transport profile of PpNCS1A and 1B differs from the recently-characterized Viridiplantae NCS1 in breadth, revealing a flexibility in solute interactions with NCS1 across plant evolution.


Assuntos
Bryopsida , Proteínas de Transporte de Nucleobases , Proteínas de Plantas , Bryopsida/genética , Bryopsida/metabolismo , Teste de Complementação Genética , Proteínas de Transporte de Nucleobases/genética , Proteínas de Transporte de Nucleobases/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo
2.
Plant Physiol Biochem ; 70: 52-60, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23770594

RESUMO

The single cell alga Chlamydomonas reinhardtii is capable of importing purines as nitrogen sources. An analysis of the annotated C. reinhardtii genome reveals at least three distinct gene families encoding for known nucleobase transporters. In this study the solute transport and binding properties for the lone C. reinhardtii nucleobase cation symporter 1 (CrNCS1) are determined through heterologous expression in Saccharomyces cerevisiae. CrNCS1 acts as a transporter of adenine, guanine, uracil and allantoin, sharing similar - but not identical - solute recognition specificity with the evolutionary distant NCS1 from Arabidopsis thaliana. The results suggest that the solute specificity for plant NCS1 occurred early in plant evolution and are distinct from solute transport specificities of single cell fungal NCS1 proteins.


Assuntos
Chlamydomonas reinhardtii/metabolismo , Genes de Plantas , Nitrogênio/metabolismo , Proteínas de Transporte de Nucleobases/metabolismo , Proteínas de Plantas/metabolismo , Purinas/metabolismo , Alantoína/metabolismo , Sequência de Aminoácidos , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Transporte Biológico , Chlamydomonas reinhardtii/genética , Evolução Molecular , Proteínas Fúngicas/metabolismo , Dados de Sequência Molecular , Proteínas de Transporte de Nucleobases/genética , Proteínas de Plantas/genética , Saccharomyces cerevisiae/metabolismo
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