RESUMO
The human Apoptosis Inducing Factor (hAIF) is a bifunctional NAD(P)H-dependent flavoreductase involved in both mitochondrial energy metabolism and caspase-independent cell death. Even though several studies indicate that both functions are redox controlled by NADH binding, the exact role of hAIF as a reductase in healthy mitochondria remains unknown. Upon reduction by NADH, hAIF dimerizes and produces very stable flavin/nicotinamide charge transfer complexes (CTC), by stacking of the oxidized nicotinamide moiety of the NAD(+) coenzyme against the re-face of the reduced flavin ring of its FAD cofactor. Such complexes are critical to restrict the hAIF efficiency as a reductase. The molecular basis of the hAIF reductase activity is here investigated by analyzing the role played by residues contributing to the interaction of the FAD isoalloxazine ring and of the nicotinamide moiety of NADH at the active site. Mutations at K177 and E314 produced drastic effects on the hAIF ability to retain the FAD cofactor, indicating that these residues are important to set up the holo-enzyme active site conformation. Characterization of P173G hAIF indicates that the stacking of P173 against the isoalloxazine ring is relevant to determine the flavin environment and to modulate the enzyme affinity for NADH. Finally, the properties of the F310G and H454S hAIF mutants indicate that these two positions contribute to form a compact active site essential for NADH binding, CTC stabilization, and NAD(+) affinity for the reduced state of hAIF. These features are key determinants of the particular behavior of hAIF as a NADH-dependent oxidoreductase.
Assuntos
Fator de Indução de Apoptose/química , Fator de Indução de Apoptose/metabolismo , Mitocôndrias/enzimologia , Sequência de Aminoácidos , Fator de Indução de Apoptose/genética , Domínio Catalítico , Sequência Conservada , Humanos , Modelos Moleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Mutação , NAD/metabolismo , Multimerização Proteica , Estrutura Quaternária de ProteínaAssuntos
Síndrome de Cockayne/genética , Códon sem Sentido , Enzimas Reparadoras do DNA/genética , Fatores de Transcrição/genética , Encéfalo/patologia , Paralisia Cerebral/diagnóstico , Síndrome de Cockayne/diagnóstico , Síndrome de Cockayne/epidemiologia , Síndrome de Cockayne/patologia , Códon/genética , Erros de Diagnóstico , Progressão da Doença , República Dominicana/etnologia , Seguimentos , Homozigoto , Humanos , Lactente , Imageamento por Ressonância Magnética , Masculino , Fenótipo , Análise de Sequência de DNARESUMO
Plastocyanin and cytochrome c6 from the green alga Scenedesmus vacuolatus were immunoquantified in cells grown under different concentrations of copper and iron. Plastocyanin expression was constitutive, its synthesis was not significantly affected by iron availability, and increases with copper availability. On the contrary, cytochrome c6 synthesis is repressed by copper, and only residual amounts of the protein were detected at 0.1 micromol/L copper. Under copper deficiency, cytochrome c6 is slightly dependent on iron. In natural environments, plastocyanin seems to be the predominant electron donor to P700.
