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Acta Cytol ; 44(2): 114-23, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10740593

RESUMO

OBJECTIVE: To compare Papanicolaou staining, enzyme immunoassay (EIA) and the polymerase chain reaction (PCR) techniques for detecting Chlamydia trachomatis in pregnant women. STUDY DESIGN: Endocervical specimens were taken randomly from 125 pregnant women with or without symptoms. These women attended their first medical consultation at the Regional General Ignacio Zaragoza Hospital. Samples were analyzed for detection of C trachomatis. When results differed between tests, specimens were evaluated by direct immunofluorescence staining. RESULTS: The prevalence of chlamydial infection was 2.4%. The characteristics of patients positive for Chlamydia were: average age, 24 years; first sexual encounter at age 21 years, one partner and six to nine months of gestation. The sensitivity, specificity, accuracy, positive predictive values and negative predictive values were 100%, 99.18%, 99.20%, 75% and 100%, respectively, for Papanicolaou staining; 100%, 92.62%, 92%, 25% and 100% for EIA; and 100%, 100%, 100% and 100% for PCR. CONCLUSION: Both Papanicolaou staining and PCR were adequate for diagnosis of C trachomatis infection. EIA was not reliable and therefore is not recommended for use as a diagnostic technique in a pregnant population with low risk and low prevalence.


Assuntos
Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis/isolamento & purificação , Complicações Infecciosas na Gravidez/diagnóstico , Adulto , Antígenos de Bactérias/análise , Infecções por Chlamydia/microbiologia , Chlamydia trachomatis/genética , DNA Bacteriano/análise , Estudos de Avaliação como Assunto , Feminino , Técnica Direta de Fluorescência para Anticorpo , Humanos , Técnicas Imunoenzimáticas/métodos , Teste de Papanicolaou , Reação em Cadeia da Polimerase/métodos , Gravidez , Complicações Infecciosas na Gravidez/microbiologia , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Esfregaço Vaginal/métodos
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