The physicochemical approaches of altering growth and biochemical properties of medicinal plants in saline soils.

Medicinal plants are important sources of biochemical compounds affecting human health. However, because large areas of the world are subjected to different stresses including salinity, it is important to find methods, which may control the growth and biochemical properties of medicinal plants in such conditions. Another aspect of cropping medicinal plants in saline soils is the alteration of their biochemical properties by stress. Due to the significance of planting medicinal plants in saline soils, the objective of the present review article is to investigate and analyze the physicochemical approaches including soil leaching, organic fertilization, mineral nutrition, ozonated water, magnetism, superabsorbent polymers, and zeolite, which may control the effects of salinity stress on the growth and biochemical properties (production of secondary metabolites) of medicinal plants. In our just-published review article, we investigated the biological approaches, which may affect the growth and biochemical properties of medicinal properties in saline soils. Although salinity stress may induce the production of biochemical products in medicinal plants, the use of physicochemical approaches is also recommendable for the improved growth and biochemical properties of medicinal plants in saline soils. More has yet to be indicated on the use of the physicochemical approaches, which may affect the growth and biochemical properties of medicinal plants in salt stress conditions. KEY POINTS: • Growth and physiological alteration of medicinal plants in salt stress conditions. • The physicochemical approaches of such alteration have been reviewed. • More has yet to be indicated on the approaches, which may affect such properties.

The biological approaches of altering the growth and biochemical properties of medicinal plants under salinity stress.

Due to their interesting properties for human health, medicinal plants are of worldwide interest, including Iran. More has yet to be investigated and analyzed on the use of methods affecting medicinal plant growth and biochemical properties under stress. The important question about medicinal plants is the purpose of their plantation, determining their growth conditions. The present review article is about the effects of salinity stress on the growth and production of secondary metabolites (SM) in medicinal plants. In stressful conditions including salinity, while the growth of medicinal plants decreases, the production of secondary metabolites (SM) may increase significantly affecting plant medicinal properties. SMs are self-protective substances that medicinal plants quickly accumulate to resist changes in the external environment. Although previous research has indicated the effects of salt stress on the growth and yield of medicinal plants, more has yet to be indicated on how the use of biological methods including plant growth regulators (PGR) and soil microbes (mycorrhizal fungi and plant growth-promoting rhizobacteria, PGPR) may affect the physiology of medicinal plants and the subsequent production of SM in salt stress conditions. The use of modern omics has become significantly important for the identification and characterization of new SM, transcriptomics, genomics, and proteomics of medicinal plants, as well as for the high production of plant-derived medicines. Accordingly, the possible biological mechanisms, which may affect such properties, have been presented. Future research perspectives for the production of medicinal plants in saline fields, using biological methods, have been suggested. KEY POINTS: • The important question about medicinal plants is the purpose of their plantation. • Secondary metabolites (SM) may significantly increase under salinity stress. • Biological methods, affecting the production of SM by stressed medicinal plants.

Genomic Research Favoring Higher Soybean Production.

Interest in the efficient production of soybean, as one of the most important crop plants, is significantly increasing worldwide. Soybean symbioses, the most important biological process affecting soybean yield and protein content, were revitalized due to the need for sustainable agricultural practices. Similar to many crop species, soybean can establish symbiotic associations with the soil bacteria rhizobia, and with the soil fungi, arbuscular mycorrhizal fungi, and other beneficial rhizospheric microorganisms are often applied as biofertilizers. Microbial interactions may importantly affect soybean production and plant health by activating different genomic pathways in soybean. Genomic research is an important tool, which may be used to elucidate and enhance the mechanisms controlling such actions and interactions. This review presents the available details on the genomic research favoring higher soybean production. Accordingly, new technologies applied to plant rhizosphere and symbiotic microbiota, root-plant endophytes, and details about the genetic composition of soybean inoculant strains are highlighted. Such details may be effectively used to enhance soybean growth and yield, under different conditions, including stress, resulting in a more sustainable production.

The Antioxidant, Anticarcinogenic and Antimicrobial Properties of Verbascum thapsus L.

BACKGROUND: The antioxidant, anticarcinogenic, and antimicrobial activities of Verbascum Thapsus L., known as great mullein, (an important medicinal plant containing different biochemical compounds including sesquiterpenes, flavonoids, saponins and lignins) were determined. There is not much data, to our knowledge, in this respect. METHODS: The antioxidant activities of V. Thapsus were investigated by the DPPH (2, 2- diphenyl- 1-picrylhydrazyl) method. Using GC-MS, the presence of different anticarcinogenic products including 1-hexzanol (2.11%), 2-hexene (1.95%), etc. was determined in the ethanolic extract of V. Thapsus. The antimicrobial activities of V. Thapsus were determined by the minimum inhibiting concentration (MIC) and minimum bactericidal concentration (MBC) methods using the Grampositive and -negative bacterial strains. RESULTS: The least concentration of V. Thapsus L. ethanolic extract (50 mg/l) resulted in only 21.26% inhibition of DPPH free radicals, however, the concentrations of 300 mg/l resulted in almost the highest inhibition (91.31%) of DPPH free radicals. The antioxidant activities of synthesized antioxidant BHT at the concentration of 300 mg/l or higher were similar to the antioxidant activities of V. Thapsus L. ethanolic extract. Both the isolated and the standard Gram-negative bacterial strains were more tolerant to the V. Thapsus ethanolic extract, compared with the Grampositive bacterial strains. Bacillus cereus was the most sensitive bacterial strain among the tested bacterial strains. CONCLUSION: The medicinal plant V. Thapsus L. can be used for the treatment of different diseases, such as cancer and infectious diseases.

Sustainable wheat (Triticum aestivum L.) production in saline fields: a review.

A large part of global agricultural fields, including the wheat (Triticum aestivum L.) ones, are subjected to various stresses including salinity. Given the increasing world population, finding methods and strategies that can alleviate salinity stress on crop yield production is of outmost importance. The presented review has consulted more than 400 articles related to the clean and sustainable production of wheat in saline fields affected by biological, environmental, economical, and social parameters including the important issue of climate change (global warming). The negative effects of salt stress on plant growth and the techniques, which have been so far detected to alleviate salinity stress on wheat growth have been analyzed and presented. The naturally tolerant species of wheat can use a range of mechanisms to alleviate salinity stress including sodium exclusion, potassium retention, and osmoregulation. However, the following can be considered as the most important techniques to enhance wheat tolerance under stress: (1) the biotechnological (crop breeding), biological (soil microbes), and biochemical (seed priming) methods, (2) the use of naturally tolerant genotypes, and (3) their combined use. The proper handling of irrigation water is also an important subject, which must be considered when planting wheat in saline fields. In conclusion, the sustainable and cleaner production of wheat under salt stress is determined by a combination of different parameters including the biotechnological techniques, which if handled properly, can enhance wheat production in saline fields.

The phytochemical variability of fatty acids in basil seeds (Ocimum basilicum L.) affected by genotype and geographical differences.

Basil (Ocimum basilicum) from the Lamiaceae family is among the most important medicinal plants, and its seed fatty acid (FA) composition and quantity affects its nutritional and health values. It was hypothesized basil species and geographical properties significantly affect seed FA composition and quantity, which has not been previously investigated, to our knowledge. The collected seeds of the 18 basil populations were planted in a farmer's field, and the seed saturated (palmitic and stearic) and unsaturated (oleic, linoleic, and linolenic) FA were determined. Shiraz1 (14.7%) and Mobarakeh (5.1%) had the highest and the least rates of total FA, respectively. The populations were significantly different in terms of saturated FA ranging from 10.73% (Ardestan) to 13.51% (Bid Zard). However, the seed unsaturated FA (expect linoleic acid) were not significantly different from each other (average = 87.27%). Basil species and geographical properties significantly affected basil saturated FA and just unsaturated linoleic acid.

Plant hormones as signals in arbuscular mycorrhizal symbiosis.

Arbuscular mycorrhizal (AM) fungi are non-specific symbionts developing mutual and beneficial symbiosis with most terrestrial plants. Because of the obligatory nature of the symbiosis, the presence of the host plant during the onset and proceeding of symbiosis is necessary. However, AM fungal spores are able to germinate in the absence of the host plant. The fungi detect the presence of the host plant through some signal communications. Among the signal molecules, which can affect mycorrhizal symbiosis are plant hormones, which may positively or adversely affect the symbiosis. In this review article, some of the most recent findings regarding the signaling effects of plant hormones, on mycorrhizal fungal symbiosis are reviewed. This may be useful for the production of plants, which are more responsive to mycorrhizal symbiosis under stress.

Soil microbes and plant fertilization.

With respect to the adverse effects of chemical fertilization on the environment and their related expenses, especially when overused, alternative methods of fertilization have been suggested and tested. For example, the combined use of chemical fertilization with organic fertilization and/or biological fertilization is among such methods. It has been indicated that the use of organic fertilization with chemical fertilization is a suitable method of providing crop plants with adequate amount of nutrients, while environmentally and economically appropriate. In this article, the importance of soil microbes to the ecosystem is reviewed, with particular emphasis on the role of plant growth-promoting rhizobacteria, arbuscular mycorrhizal fungi, and endophytic bacteria in providing necessary nutrients for plant growth and yield production. Such microbes are beneficial to plant growth through colonizing plant roots and inducing mechanisms by which plant growth increases. Although there has been extensive research work regarding the use of microbes as a method of fertilizing plants, it is yet a question how the efficiency of such microbial fertilization to the plant can be determined and increased. In other words, how the right combination of chemical and biological fertilization can be determined. In this article, the most recent advances regarding the effects of microbial fertilization on plant growth and yield production in their combined use with chemical fertilization are reviewed. There are also some details related to the molecular mechanisms affecting the microbial performance and how the use of biological techniques may affect the efficiency of biological fertilization.

Arbuscular mycorrhizas enhance nutrient uptake in different wheat genotypes at high salinity levels under field and greenhouse conditions.

Since most experiments regarding the symbiosis between arbuscular mycorrhizal (AM) fungi and their host plants under salinity stress have been performed only under greenhouse conditions, this research work was also conducted under field conditions. The effects of three AM species including Glomus mosseae, G. etunicatum and G. intraradices on the nutrient uptake of different wheat cultivars (including Roshan, Kavir and Tabasi) under field and greenhouse (including Chamran and Line 9) conditions were determined. At field harvest, the concentrations of N, Ca, Mg, Fe, Cu, and Mn, and at greenhouse harvest, plant growth, root colonization and concentrations of different nutrients including N, K, P, Ca, Mg, Mn, Cu, Fe, Zn, Na and Cl were determined. The effects of wheat cultivars on the concentrations of N, Ca, and Mn, and of all nutrients were significant at field and greenhouse conditions, respectively. In both experiments, AM fungi significantly enhanced the concentrations of all nutrients including N, K, P, Ca, Mg, Mn, Cu, Fe, Zn, Na and Cl. The synergistic and enhancing effects of co-inoculation of AM species on plant growth and the inhibiting effect of AM species on Na(+) rather than on Cl(-) uptake under salinity are also among the important findings of this research work.

Hyperaccumulators, arbuscular mycorrhizal fungi and stress of heavy metals.

Use of plants, with hyperaccumulating ability or in association with soil microbes including the symbiotic fungi, arbuscular mycorrhiza (AM), are among the most common biological methods of treating heavy metals in soil. Both hyperaccumulating plants and AM fungi have some unique abilities, which make them suitable to treat heavy metals. Hyperaccumulator plants have some genes, being expressed at the time of heavy metal pollution, and can accordingly localize high concentration of heavy metals to their tissues, without showing the toxicity symptoms. A key solution to the issue of heavy metal pollution may be the proper integration of hyperaccumulator plants and AM fungi. The interactions between the soil microbes and the host plant can also be important for the treatment of soils polluted with heavy metals.

The effects of selenium and other micronutrients on the antioxidant activities and yield of corn (Zea mays L.) under drought stress.

The effects of selenium (Se) on plant growth under drought stress and in the presence of micronutrients are yet to be investigated. Hence, in a field experiment in 2007 the effects of Se and micronutrients including iron (Fe), zinc (Zn), copper (Cu), manganese (Mn), boron (B) and molybdenum (Mo) were evaluated on corn (Zea mays L.) grain yield under drought stress. Main- and sub-plots were devoted to irrigation (control and water stressed at the eight-leaf, blister and grain filling stages) and micronutrients treatments, respectively. Micronutrients were foliarly applied at 2 l ha(-1) at the six-leaf stage, one week before tasseling, using a corn fertilizer, called biomin containing (on the basis of dry weight percentage) Fe (2.6), Zn (4.1), Cu (1.5), Mn (2.6), B (1.5), Mo (0.5) and Mg (4.1). Se was used as sodium selenite (Na2SeO3), at the rate of 20 g ha(-1) two weeks before treating the plants with drought stress. Effects of drought stress on plant growth were determined based on the activity or level of antioxidants. With increasing the stress level, addition of Se or micronutrients significantly enhanced the antioxidant activity and level as well as corn grain yield. The interaction effects between Se and micronutrients adversely affected antioxidant activity as well as corn grain yield. Se addition at the grain filling stage resulted in the highest grain yield under drought stress. The single but not the combined use of Se or micronutrients can alleviate the unfavorable effects of drought stress on corn yield by affecting plant metabolism including antioxidant activity.

Arbuscular mycorrhizal fungi and nitrogen uptake.

Nitrogen (N) is among the most important macro-nutrients significantly affecting plant growth and yield production. Accordingly, N must be supplied adequately so that optimum amounts of yield are resulted. There are different ways of supplying N to the plant including the use of chemical and biological fertilization. The chemical properties of N make it very mobile, especially under humid conditions. Hence, N must not be overfertilized with respect to the economical and environmental points of view. N Biological fertilization includes the use of plant growth-promoting rhizobacteria (PGPR) including the N-fixing bacteria, rhizobium. There are also arbuscular mycorrhizal (AM) fungi in the soil, which are symbiotic to most terrestrial plants enhancing plant growth and yield production through increasing the uptake of water and nutrients by the host plant. Numerous experiments have indicated the important role of AM fungi in enhancing P uptake by plant. However, it is yet a matter of debate that how AM fungi may affect soil N dynamic and hence plant N uptake. Some of the most important and recent aspects regarding such effects by AM fungi are highlighted, which can be of significance to health and productivity of the ecosystem.

Interactions between arbuscular mycorrhizal fungi and soil bacteria.

The soil environment is interesting and complicated. There are so many interactions taking place in the soil, which determine the properties of soil as a medium for the growth and activities of plants and soil microorganisms. The soil fungi, arbuscular mycorrhiza (AM), are in mutual and beneficial symbiosis with most of the terrestrial plants. AM fungi are continuously interactive with a wide range of soil microorganisms including nonbacterial soil microorganisms, plant growth promoting rhizobacteria, mycorrhiza helper bacteria and deleterious bacteria. Their interactions can have important implications in agriculture. There are some interesting interactions between the AM fungi and soil bacteria including the binding of soil bacteria to the fungal spore, the injection of molecules by bacteria into the fungal spore, the production of volatiles by bacteria and the degradation of fungal cellular wall. Such mechanisms can affect the expression of genes in AM fungi and hence their performance and ecosystem productivity. Hence, consideration of such interactive behavior is of significance. In this review, some of the most important findings regarding the interactions between AM fungi and soil bacteria with some new insights for future research are presented.

Fatty acid composition of canola (Brassica napus L.), as affected by agronomical, genotypic and environmental parameters.

Vegetable oils with a high relative amount of unsaturated fatty acids are of great significance for human health. There is not any data on the effects of tillage practices on fatty acid composition of canola (Brassica napus L.). Hence, in a 2-year split plot experiment, the effects of different tillage systems (no (NT), minimum (MT) and conventional tillage (CT)), canola genotypes (Hyola 401 (V1) and PF (V2)) and sowing dates (including Sep. 8, 23 and Oct. 7) on the fatty acid composition of canola were evaluated. Tillage practices and the combination of canola genotypes and sowing dates were randomized to the main and sub-plots, respectively. The highest oleic acid content was the result of combining NT, V1 and Sep. 23, and the lowest was related to the combination of CT, V2 and Oct. 7. While the combination of NT, V1 and D1 resulted in the highest amount of unsaturated fatty acids, this amount was the lowest for the combination of CT, V2 and Sep. 23. For the selection of an appropriate canola producing strategy, all these parameters must be taken into account. The combination of NT, V1 and Sep. 23 may be the most favorable cropping strategy for canola production under a Mediterranean climate.

Yield and yield components of hybrid corn (Zea mays L.) as affected by mycorrhizal symbiosis and zinc sulfate under drought stress.

With respect to the significance of improving hybrid corn performance under stress, this experiment was conducted at the Islamic Azad University, Arak Branch, Iran. A complete randomized block design with three levels of irrigations (at 100%, 75% and 50% crop water requirement), two levels of arbuscular mycorrhizal (AM) fungi (Glumus intraradisis) (including control), and three levels of zinc (Zn) sulfate (0, 25 and 45 kg ha(-1)), was performed. Results of the 2-year experiments indicated that irrigation treatment significantly affected corn yield and its components at P = 1%. AM fungi and increasing Zn levels also resulted in similar effects on corn growth and production. Although AM fungi did not significantly affect corn growth at the non-stressed irrigation treatment, at moderate drought stress AM fungi significantly enhanced corn quality and yield relative to the control treatment. The combined effects of AM fungi and Zn sulfate at 45 kg ha(-1) application significantly affected corn growth and production. In addition, the tripartite treatments significantly enhanced corn yield at P = 1%. Effects of Zn and AM fungi on plant growth under drought stress is affected by the stress level.

Isolation and characterization of ACC deaminase-producing fluorescent pseudomonads, to alleviate salinity stress on canola (Brassica napus L.) growth.

Salinity stress is of great importance in arid and semi-arid areas of the world due to its impact in reducing crop yield. Under salinity stress, the amount of 1-aminocyclopropane-1-carboxylate (ACC), a precursor for ethylene production in plants, increases. Here, we conducted research under the hypothesis that isolated ACC deaminase-producing Pseudomonas fluorescens and Pseudomonas putida can alleviate the stressful effects of salinity on canola (Brassica napus L.) growth. The experiments were conducted in the Soil and Water Research Institute, Tehran, Iran. Seven experimental stages were conducted to isolate and characterize ACC deaminase-producing Pseudomonas fluorescens strains and to determine factors enhancing their growth and, consequently, their effects on the germination of canola seeds. Under salinity stress, in 14% of the isolates, ACC deaminase activity was observed, indicating that they were able to utilize ACC as the sole N-source. Bacterial strains differed in their ability to synthesize auxin and hydrogen cyanide compounds, as well as in their ACC deaminase activity. Under salinity stress, the rate of germinating seeds inoculated with the strains of ACC deaminase-producing Pseudomonas fluorescens and Pseudomonas putida, and seedling growth was significantly higher. These results indicate the significance of soil biological activities, including the activities of plant growth-promoting bacteria, in the alleviation of soil stresses such as salinity on plant growth.