Increased body weight in C57BL/6 female mice after exposure to ionizing radiation or 60Hz magnetic fields.

PURPOSE: The purpose of this investigation was to determine whether early treatment with ionizing radiation and/or chronic magnetic field (MF) exposure affected body weight in female mice. MATERIALS AND METHODS: Weanling C57BL/6 female mice were irradiated with four equal weekly cobalt-60 exposures (total cumulative doses: 3.0, 4.0, 5.1Gy) and/or received chronic lifetime exposure to 1.4 mT 60 Hz circularly polarized MF or ambient MF. The body weights of 2280 mice were recorded at 35 age intervals, and analysis of variance was used to compare the mean differences from baseline weights between treatment groups and sham-exposed controls. RESULTS: A highly statistically significant effect of ionizing radiation on body weight was observed at 28 age intervals (p < or = 0.001), and for MF exposure at 10 age intervals (p < or = 0.001). During the young adult growth phase, mice exposed only to MF exhibited < or =0.5 g greater weight gain relative to sham-exposed controls (p = 0.0001). The effect of ionizing radiation alone was inversely related to dose, with the largest weight increases observed in all of the irradiated groups after 9-12 months (p = 0.0001). CONCLUSIONS: Treatment with split-dose ionizing radiation at an early age and chronic exposure to a residential power frequency MF were found to produce small but significant increases in body weight.

Hematopoietic neoplasia in C57BL/6 mice exposed to split-dose ionizing radiation and circularly polarized 60 Hz magnetic fields.

This study assessed the effect of chronic exposure to a 60 Hz circularly polarized magnetic field on the occurrence of ionizing radiation-induced lymphoma and other hematopoietic neoplasia in mice. Female C57BL/6 mice received lifetime exposure to either a magnetic field flux density of 1.42 mT for 18 h/day, or an ambient magnetic field of 0.13 microT. Beginning on the first day of magnetic field exposure, 1710 mice were treated with one of three levels of split-dose Cobalt-60 gamma-radiation (cumulative 3.0, 4.0 or 5.1 Gy). The remaining 570 mice received sham irradiation treatment. Sections from 10 lymphoid tissues were evaluated histopathologically for hematopoietic neoplasia. The primary statistical analysis used the Poly3 method to compare lymphoma incidences in magnetic field (MF)-exposed and control mice. Secondary analyses used the Cox proportional hazards model to analyze incidence rates for mortality and development of specific types of neoplasia. The mortality incidence rate was increased by ionizing radiation treatment, and all neoplasms were observed sooner in irradiated mice. However, the lifetime incidence of hematopoietic neoplasia was similar in all experimental groups, including those that were not exposed to ionizing radiation. Chronic exposure to MFs did not affect the mortality incidence rates and did not change the relative incidences of hematopoietic neoplasia in mice that received the same ionizing radiation treatment, with the exception of a marginally significant reduced relative risk of 0.97 (P = 0.05) for lymphoblastic lymphoma in mice exposed to a magnetic field and treated with 5.1 Gy. Lymphomas and histiocytic sarcomas were first observed approximately 50 days sooner in mice that were exposed to magnetic fields but not ionizing radiation, although this comparison was not statistically significant and the incidence of hematopoietic neoplasia in these mice was not different from that of mice in the 0 T/0 Gy group.

Determination of organ area. Application to thyroid.

A method has been developed for the quantification of a projected organ area using a standard gamma camera with pinhole collimation and computer processing of two vertically displaced images. Formulae have been derived expressing the uniquely determined quadratic relationship between object area and aperture distance with respect to projective image areas. This technique is extremely simple and its successful application to thyroid imaging is described.

Biplanar cardiac blood-pool tomography.

Dynamic transverse axial wall tomograms of the left ventricle (LV) are reconstructed by a new technique from anterior and LAO views acquired with a conventional scintillation camera imaging the distribution of in-vivo Tc-99m-labeled red blood cells. By confining reconstruction to the singular contiguous uniform concentration of activity in the LV, the requisite angular samplings for a given level of accuracy are substantially reduced in this restricted form of emission computed tomography (ECT). Static phantom studies using a series of volumes having various cross-sectional dimensions demonstrate tomographic edge reconstruction with less than or equal to 12% rms radial error. The dynamic cardiac ECT is demonstrated in a series of representative patient studies by reconstruction of wall tomograms in the end-diastolic and end-systolic phases of the 28-frame cardiac cycle. In contrast to the conventional dual multiframe projection views, the motion tomograms derived from the reconstructions clearly show the complete three-dimensional perspective of wall displacement.

Left-ventricular ejection fraction and segmental wall motion by peripheral first-pass radionuclide angiography.

A computerized edge-detection method was developed to obtain radionuclide ventriculograms for analysis of left-ventricular ejection fraction and segmental wall motion from first-pass studies following i.v. injection of radionuclide. The accuracy of this technique was examined in 21 patients undergoing cardiac catheterization. Tc-99m DTPA was injected into an antecubital vein, with data acquisition in the 30 degrees RAO projection by a gamma scintillation camera interfaced to a computer. A computerized profile analysis was used to determine objectively the edge of the left-ventricular blood pool. Time-activity curves were generated, and the ejection fraction was calculated from sequential end-diastolic and end-systolic count rates. The values for ejection fraction correlated well with those obtained by single-plane contrast ventriculography (r = 0.95). End-diastolic and end-systolic images were reconstructed from the time-activity curve. To analyze segmental wall motion, the left-ventricular outline was divided into five segments and the motion of each segment was graded qualitatively from 1 to 5. Seventy-five of 105 segments had the same grade as the wall motion determined by contrast angiography, and 102 of 105 were within one grade. (P less than 0.001). These findings demonstrate the accuracy of this improved technique for objective, rapid, and noninvasive determination of left-ventricular function.