RESUMO
In this work, a new magnetic nanocomposite was prepared for the specific isolation of CD45 positive cells. Acrolein (AC) and acrylated ß-cyclodextrin (ACD) were used as monomers in order to polymerize on the surface of Fe3O4 magnetic nanoparticles (MNPs) via surface initiated radical polymerization. Polyacrolein-ß-CD (PACD) functionalized Fe3O4 MNPs (Fe3O4@PACD) was achieved and subsequently, fluorescein conjugated IgG anti-human CD45 was immobilized onto Fe3O4@PACD. Antibody conjugation onto the latter nanocomposite was accomplished according to IgG encapsulation in cyclodextrin cavity and Schiff base formation between aldehyde groups of MNPs and primary amines of antibody. The characterization analysis of the bare Fe3O4, Fe3O4@PACD nanocomposite, and Fe3O4@PACD-Ab were investigated using XRD, VSM, FT-IR, H-NMR, TGA, SEM, TEM, and flow cytometry techniques. The results showed that the antibody has been successfully immobilized onto Fe3O4@PACD. Moreover, the fabricated nanocomposite was used for selective capturing of CD45+ cells among other peripheral blood mononuclear cells (PBMCs). The results of TEM and fluorescence microscopy confirmed the ability of the conjugated system for efficient cell capturing.
