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1.
Microb Pathog ; 136: 103708, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31491552

RESUMO

IL-33 has powerful immunoregulatory activities such as reinforcement of Th2 cell responses. The aim was to assess the circulating IL-33 levels and IL-33 rs1929992 polymorphism in H. pylori-infected peptic ulcer (PU) patients and asymptomatic (AS) subjects. Blood samples were obtained from 100 PU patients, 100 AS subjects and 100 uninfected individuals. Circulating IL-33 levels were detected by ELISA. After DNA extraction, the IL-33 rs1929992 polymorphism was determined using PCR-RFLP method. Serum IL-33 quantities were significantly lower in PU patients compared with AS and uninfected groups. IL-33 levels were higher in AS subjects compared with uninfected group. In PU, AS and uninfected groups, IL-33 levels were significantly higher in women than men. In PU and AS groups, the CagA+H. pylori-infected subjects exhibit higher IL-33 levels compared with carriers of CagA-H. pylori strains. In PU patients, the frequency of genotype GG and allele G at IL-33 rs1929992 was significantly higher compared with all healthy subjects (AS + uninfected groups). The presence of genotypes GG and AG, and allele G in rs1929992 conferred greater risk for PU. In whole H. pylori-infected population (PU + AS groups), IL-33 levels in individuals with genotype AA or allele A at rs1929992 were higher than subjects with GG genotype or allele G. The reduced IL-33 production could contribute to the PU development during H. pylori infection. The IL-33 levels may be affected by individual gender, rs1929992 polymorphism, and the CagA status of bacteria. The rs1929992-related GG genotype and G allele may be associated with PU development.


Assuntos
Antígenos de Bactérias/genética , Proteínas de Bactérias/genética , Infecções por Helicobacter/genética , Infecções por Helicobacter/patologia , Interleucina-33/sangue , Interleucina-33/genética , Úlcera Péptica/patologia , Polimorfismo Genético , Adulto , Doenças Assintomáticas , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Fatores Sexuais
2.
Helicobacter ; 23(4): e12501, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29938865

RESUMO

BACKGROUND: IL-35 modulates immune and inflammatory responses during infections. Here, we investigated IL-35 levels and a single nucleotide polymorphism, rs3761548, in FOXP3 gene in Helicobacter pylori-infected patients with peptic ulcer (PU), to clarify possible associations. MATERIALS AND METHODS: This study includes 100 H. pylori-infected PU patients, 100 H. pylori-infected asymptomatic subjects (AS), and 100 noninfected healthy subjects (NHSs). Serum IL-35 levels and the genotyping were determined using ELISA and RFLP-PCR methods, respectively. RESULTS: In PU patients, the IL-35 levels were lower than AS and NHS groups (P < .001). The IL-35 levels in CagA+ H. pylori-infected participants from PU and AS groups were lower than individuals infected with CagA- strains (P < .02 and P < .04, respectively). Women had higher IL-35 levels than men among PU, AS, and NHS groups (P < .0001). In PU patients, AA genotype and A allele at rs3761548 were more frequent than total healthy subjects (AS + NHS groups) and associated with an increased PU risk (AA genotype: OR = 5.51, P < .0001; A allele: OR = 3.857, P < .002). In PU and AS groups, IL-35 levels were lower in subjects displaying AA genotype or A allele than subjects displaying CC genotype or C allele, respectively (P < .0001 and P < .03 for PU patients; P < .001 and P < .02 for AS group, respectively). CONCLUSIONS: Decreased IL-35 levels could be involved in PU development in H. pylori-infected individuals. IL-35 levels are affected by CagA status of H. pylori, participants gender, and genetic variations at rs3761548. The AA genotype and A allele at rs3761548 could represent a risk factor for PU development.


Assuntos
Antígenos de Bactérias/metabolismo , Proteínas de Bactérias/metabolismo , Fatores de Transcrição Forkhead/genética , Infecções por Helicobacter/sangue , Infecções por Helicobacter/genética , Helicobacter pylori/metabolismo , Interleucinas/sangue , Úlcera Péptica/sangue , Úlcera Péptica/genética , Polimorfismo Genético , Adulto , Antígenos de Bactérias/genética , Proteínas de Bactérias/genética , Feminino , Infecções por Helicobacter/microbiologia , Helicobacter pylori/genética , Humanos , Subunidade p35 da Interleucina-12/sangue , Masculino , Pessoa de Meia-Idade , Antígenos de Histocompatibilidade Menor/sangue , Úlcera Péptica/microbiologia , Fatores Sexuais
3.
Turk J Gastroenterol ; 29(3): 283-291, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29755012

RESUMO

BACKGROUND/AIMS: Toll-like receptors (TLRs), particularly TLR2 and TLR4, take part in elicitation of immune responses against Helicobacter pylori (H. pylori). This study aimed to investigate the relationship between single nucleotide polymorphisms (SNP) rs3804099 in the TLR2 gene and rs4986790 in the TLR4 gene with H. pylori infection and peptic ulcer (PU). MATERIALS AND METHODS: Blood specimens were obtained from 350 individuals, including 100 H. pylori-infected patients with PU, 125 H. pylori-infected asymptomatic subjects (AS), and 125 non-infected healthy subjects (NHS). The DNA was extracted, and the SNPs were determined using ARMS-PCR method. RESULTS: The frequency of CT genotype at TLR2 SNP rs3804099 in both the PU and AS groups was significantly higher than in the NHS group (p<0.05). In total H. pylori-infected individuals (PU+AS), the frequency of the CT genotype at rs3804099 was also significantly higher than in the NHS group (p<0.005). The frequency of the CC genotype at rs3804099 in PU+AS was markedly lower than in the NHS group (p=0.066). PU patients carried CT genotype more frequently than total healthy individuals (AS+NHS) (p<0.03). The distribution of the TT genotype was lower, whereas the frequency of the CT genotype was higher in AS individuals infected with CagA+ strains than those infected with CagA- strains (p<0.03). No significant differences were found among the PU, AS, and NHS groups regarding the genetic differences at rs4986790 in the TLR4 gene. CONCLUSION: These results provide evidence regarding the association of the rs3804099 in the TLR2 gene with H. pylori infection and PU. The rs3804099 may affect vulnerability to H. pylori infection, particularly to CagA+ strains of bacteria.


Assuntos
Infecções por Helicobacter/genética , Helicobacter pylori , Úlcera Péptica/genética , Polimorfismo de Nucleotídeo Único , Receptor 2 Toll-Like/genética , Adulto , Antígenos de Bactérias/genética , Proteínas de Bactérias/genética , Estudos de Casos e Controles , Feminino , Genótipo , Helicobacter pylori/genética , Helicobacter pylori/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , Úlcera Péptica/microbiologia , Receptor 4 Toll-Like/genética
4.
Addict Health ; 3(1-2): 61-7, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-24494118

RESUMO

BACKGROUND: Addiction in pregnant women causes complications such as abortion, asphyxia and cerebral and physical problems. APGAR score assesses vital signs and birth weight and represents the physical and brain growth of newborns. In this study, the effects of opium addiction in mothers on birth weight and APGAR scores of neonates were discussed. METHODS: This study analytic, descriptive study was conducted on 49 pregnant women addicted to oral consumption of opium (0.5-0.8 grams daily) and 49 non-addicted women who referred to Afzalipour Hospital associated with Kerman University of Medical Sciences. Information including various personal characteristics, history of addiction and drug consumption, and the possibility of taking other drugs was collected by a researcher and recorded confidentially in a checklist. Birth weight and APGAR score t first, fifth and tenth minutes were also recorded. Statistical analysis was performed using Pearson correlation test, independent t-test, and repeated measure to evaluate the APGAR scores and other characteristics of the two groups of infants. FINDINGS: Average birth weight of infants with addicted mothers was 2255 grams which had a significant difference with infants born by non-addicted mothers (P < 0.0001). Average APGAR scores at the first minute were 7.6 ± 1.1 and 8.6 ± 1.1 among infants from addicted and non-addicted mothers, respectively. Average APGAR scores over time (at minutes 1, 5 and 10) had a significant difference (P < 0.0001) where an ascending trend was seen. This difference was significant in both groups (P = 0.003). CONCLUSION: Drug addiction in mothers decreases the APGAR score and birth weight of infants.

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