RESUMO
Electrophoretic spectra of multiple molecular forms of peroxidase (EC 1.11.1.7), superoxide dismutase (EC 1.15.1.1), phenol oxidase (EC 1.10.3.1), and cytochrome oxidase (EC 1.9.3.1) in seedlings of two aegilops species and eleven genotypes of bread winter wheat differing in the level of their resistance to Fusarium infection are presented. Several izoforms of peroxidase, phenol oxidase, and superoxide dismutase correlate with the level of resistance to Fusarium. Infection of plants with the pathogen enhances expressiveness of some multiple forms of enzymes. Such response to infection in less pronounced in the sensitive genotypes as compared with that in the resistant ones.
Assuntos
Fusarium/patogenicidade , Isoenzimas/metabolismo , Triticum/enzimologia , Complexo IV da Cadeia de Transporte de Elétrons/química , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Eletroforese em Gel de Poliacrilamida , Genótipo , Imunidade Inata , Isoenzimas/química , Monofenol Mono-Oxigenase/química , Monofenol Mono-Oxigenase/metabolismo , Micoses/metabolismo , Peroxidase/química , Peroxidase/metabolismo , Doenças das Plantas/microbiologia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Sementes/enzimologia , Superóxido Dismutase/química , Superóxido Dismutase/metabolismo , Triticum/imunologiaRESUMO
Potato plants of various cultivars, transformed using Agrobacterium tumefaciens with pGV941 plasmid, differed from control plants in glyphosate herbicide tolerance, tryptophane content, intensity of callusogenesis, microtuber formation in vitro and multimolecular forms of peroxidase (EC 1.11.1.7) and superoxide dismutase (EC 1.15.1.11). The results demonstrate the influence of alien DNA on structural gene expression in transgenic plants.
Assuntos
Solanum tuberosum/genética , Transformação Genética , Agrobacterium tumefaciens/genética , Herbicidas/toxicidade , Isoenzimas/metabolismo , Peroxidase/metabolismo , Solanum tuberosum/química , Solanum tuberosum/enzimologia , Superóxido Dismutase/metabolismo , Transgenes , Triptofano/análiseRESUMO
A genetic basis of resistance of winter wheat to Fusarium graminearum causing Fusarium head blight was defined as a result of F1, F2, BC1 hybrid analysis in the crosses of some lines and varieties with highly susceptible variety Odesskaya polukarlikovaya. It was found out that resistance to Fusarium graminearum inherited regardless of resistance to rust, mildew and Septoria.
