Use of continuous wavelet transform approach for simultaneous quantitative determination of multicomponent mixture by UV-Vis spectrophotometry.

In the present paper, a multicomponent analysis approach based on spectrophotometry method was developed for simultaneous determination of Guaifenesin (GU), Chlorpheniramine (CHL) and Pseudoephedrine (PSE) without any separation steps. The method under study is signal processing method based on Continuous Wavelet Transform (CWT) coupled with zero cross point technique. In this paper, CWT method was tested by synthetic ternary mixtures and was applied to the commercial cough syrup as a real sample and assessed by applying the standard addition technique. For demonstrate the accuracy of the results, other applications of signal processing, such as Derivative Transform (DT), Partial Least Squares (PLS) regression and Principal Components Regression (PCR) were used as comparative methods. Afterwards, the obtained results from analyzing the cough syrup by all methods were compared to the High-Performance Liquid Chromatography (HPLC) as a reference method. One-way analysis of variance test at 95% confidence level showed no significant differences between CWT and other applications.

Chemometric simultaneous determination of Sofosbuvir and Ledipasvir in pharmaceutical dosage form.

Partial least squares (PLS), different families of continuous wavelet transform (CWT), and first derivative spectrophotometry (DS) techniques were studied for quantification of Sofosbuvir (SFB) and Ledipasvir (LDV) simultaneously without separation step. The components were dissolved in Acetonitrile and the spectral behaviors were evaluated in the range of 200 to 400nm. The ultraviolet (UV) absorbance of LDV exhibits no interferences between 300 and 400nm and it was decided to predict the LDV amount through the classic spectrophotometry (CS) method in this spectral region as well. Data matrix of concentrations and calibrated models were developed, and then by applying a validation set the accuracy and precision of each model were studied. Actual concentrations versus predicted concentrations plotted and good correlation coefficients by each method resulted. Pharmaceutical dosage form was quantified by developed methods and the results were compared with the High Performance Liquid Chromatography (HPLC) reference method. Analysis Of Variance (ANOVA) in 95% confidence level showed no significant differences among methods.