RESUMO
The process of collagen and proteoglycan synthesis in the in vitro cultured fibroblasts was studied by four original histoenzymological methods: UDP-glucuronic-5-epimerase, hydroxyproline epimerase, UDP-4-epimerase and UDP-N-acetyl-glucosamine epimerase. The process of epimerasization varied in terms of the cell growth stages. Synthesis of collagen and proteoglycans developed in the cells of the same culture but with various intensities in different cell groups.
Assuntos
Tecido Conjuntivo/enzimologia , Fibroblastos/enzimologia , Isomerases/análise , Isomerases/metabolismo , Racemases e Epimerases/análise , Racemases e Epimerases/metabolismo , Animais , Células Cultivadas/enzimologia , Embrião de Galinha , Colágeno/biossíntese , Fibroblastos/metabolismo , Fibroblastos/ultraestrutura , Proteoglicanas/biossínteseRESUMO
By means of a histochemical method, the leucine aminopeptidase activity was studied in human normal and leukemic peripheral blood. Before administration of cytostatics the enzymic activity was high in the cytoplasm of blasts and in the nuclei of segmented cells. After therapy the activity of leucine aminopeptidase in blasts decreased, while in polymorphs it became negative in nuclei and positive in their cytoplasm. The non-granular leukocytes and platelets displayed a weak enzymic activity both in normal and malignant blood cells. The erythrocytes were negative.
Assuntos
Leucil Aminopeptidase , Leucemia Mieloide Aguda/enzimologia , Leucemia Mieloide/enzimologia , Antineoplásicos/uso terapêutico , Células Sanguíneas/enzimologia , Histocitoquímica , Humanos , Leucemia Mieloide/tratamento farmacológicoRESUMO
Human, monkey, cat and rat bone marrow cells were studied for the histochemical localization of leucine aminopeptidase activity. The granulocytes have a weak enzymic activity in the immature cells and a very strong one in the differentiated cells. The lymphopoiesis is characterized by a strong reaction in the lymphoblasts and different degrees of the enzyme activity in the lymphocytes. In the erythropoiesis, the undifferentiated cells are strongly positive, while the erythrocytes are negative. The immature and mature cells of the megakariocytic line show a weak leucine aminopeptidase activity.
Assuntos
Células da Medula Óssea , Medula Óssea/enzimologia , Leucil Aminopeptidase/metabolismo , Fatores Etários , Animais , Gatos , Haplorrinos , Humanos , RatosRESUMO
The activity of dihydrofolate dehydrogenase by histochemical method in children leukocytes was investigated. Blood leukocytes have a positive enzymic activity. Except monocytes a large enzymic variability was observed in neutrophils, eosinophils, and lymphocytes in puberty and at the onset of the adolescence. Enzymic activity varies with blood cellular type, the age, in males and females and from one child to another.
