STR data for the AmpFlSTR profiler plus loci from Majorcan, Minorcan and Valencian populations (Eastern Spain).

Frequency data of the nine STRs included in the AmpFlSTR profiler plus kit were determined in a sample of 304 unrelated individuals from three Spanish populations: 103 from Majorca, 100 from Minorca, and 101 from Valencia.

Lesions of the triangular fibrocartilage in fractures of the wrist.

MRI conducted in 32 cases of distal fracture of the radius a mean of 93 days after trauma allowed for the identification of various lesions of the soft tissues among which those of the triangular fibrocartilaginous complex. These observations confirm the presence of lesions of the triangular fibrocartilaginous complex (TFC) among immediate complications of distal fractures of the radius and the diagnostic role of MRI in post-traumatic ulnar pain.

[Neonatal hemoperitoneum: role of ultrasonography. Experience with 4 cases]. / Emoperitoneo neonatale: ruolo dell'ecografia. Esperienza in quattro casi.

This study was aimed at investigating the role of US in the newborn with a clinical suspicion of hemoperitoneum. Hemoperitoneum is a dramatic clinical event which may be caused by some obstetrical and fetal factors--breech or complicated delivery, hepatomegaly, macrosomy, anoxic liver congestion being the most frequent causes. Direct or indirect trauma of an abdominal organ during delivery represents the major pathogenetic factor. Clinical symptoms are correlated with the appearance of shock whose intensity is proportional to trauma severity: symptoms may appear even 48 hours after delivery. The authors report their experience with 4 consecutive cases of neonatal hemoperitoneum which underwent US of the abdomen 24 to 72 hours after birth. In the first 3 newborns, US was performed on the basis of a clinical picture of bleeding shock, while in the extant newborn US was performed after a sudden increase in blood transaminase level associated with an obstetrical paralysis of the left arm. The US examinations were performed using a 7.5-MHz sectorial probe unit (Siemens). In all patients, US demonstrated the presence of a liquid effusion in the abdomen which could be referred to hemoperitoneum. In two patients, it was associated with a traumatic focal alteration of the liver parenchyma, in the third with a diffuse subcapsular liver hematoma and, finally in the fourth patient with a disconnected splenic vascular pedicle which was at surgery demonstrated. In conclusion, US is indicated as the most reliable and valuable diagnostic technique in the study of neonatal hemoperitoneum. The authors believe abdominal US to be essential in all the neonatal cases where hemoperitoneum is suspected and in selected cases presenting specific risk factors, to allow a rapid diagnosis and better treatment planning.

MR findings in post-traumatic carpal tunnel syndrome.

Stenosis of the canal secondary to poor consolidation of fractures of the distal radial epiphysis is one of the causes of compression on the median nerve of the wrist. Other post-traumatic compressive pathologies of the median nerve when there are no significant skeletal modifications caused by probable involvement of the soft tissues surrounding and within the canal are also described. MR was used to study 23 patients affected with the sequelae of fracture of the radial distal epiphysis who presented with clinical and electromyographic signs of carpal tunnel syndrome, and were submitted to decompressive surgery. MR showed indirect signs of compression, such as morphological changes of the median nerve, as well as post-traumatic changes in the carpal canal. MR allows for a complete anatomical view of the canal structures implicated in causing post-traumatic carpal tunnel syndrome.

MR imaging of ulcerative colitis.

High-resolution magnetic resonance imaging (MRI) was used to study 16 resected rectosigmoid specimens of patients treated with total colectomy for severe ulcerative colitis (UC). Six normal colon specimens were also studied as a control group. Moreover, a parallel study of the pelvis of 24 patients with a proven diagnosis of UC was performed with the same MR system. Both in vitro and in vivo MRI findings [thickening and signal intensity (SI)] of the mural layers were qualitatively evaluated by two radiologists and compared with gross and microscopic aspects. In vitro results showed that MRI was able to identify all layers of the colonic wall. In particular in UC specimens, MRI identified thickening and the peculiar abnormal hyperintensity of the mucosal and submucosal layers on spin-echo (SE) T1-weighted images. In vivo results confirmed the high-signal intensity of the mucosal and submucosal layers. These findings were not observed in the control group in which the superficial layers appeared low in intensity on SE T1 images. Our preliminary experience suggests that MRI should be considered a new imaging modality for detecting UC colonic wall changes.

[Magnetic resonance in the diagnosis of lesions of the carpal triangular fibrocartilage. The experience with 49 patients with chronic pain at the wrist]. / La Risonanza Magnetica nella diagnosi delle lesioni della fibrocartilagine triangolare del carpo. Esperienza su 49 pazienti con dolore cronico al polso.

In order to evaluate the diagnostic capabilities of MR Imaging in defining triangular fibrocartilage (TFC) lesions, 49 selected patients--21 with rheumatoid arthritis, 13 with traumatic injury and 15 with wrist fracture--presenting chronic wrist pain were examined with MR Imaging. The wrists of 12 healthy subjects were also studied as a control group. MR examinations were performed by means of a superconductive 1.0 T unit (Magnetom-Siemens); SE and GE T1- and T2-weighted pulse sequences were employed to acquire images of the wrists on the coronal, axial and, if necessary, sagittal planes. The results showed peripheral TFC tears in 10 cases, central TFC lesions in 12 cases and TFC lesions with collateral insertion involvement in 9 cases. In 4 patients focal thickening of TFC without disruption was observed. The correct diagnosis was made in all the 19 surgically verified cases. Our experience confirms the clinical value of MR Imaging in the evaluation of TFC lesions in patients with chronic ulnar pain syndrome.

Evaluation of the pulmonary artery by cine MRI.

Twenty-four patients whose mediastinal (12 patients) and lung (12 patients) neoplasms were eventually verified were evaluated with cine MRI in addition to conventional methods (plain radiography, CT, MRI, and endoscopy). Using a 1.0 T system and gradient echo (FLASH repetition time 250-350 ms, echo time 12, flip angle 25-60 degrees) multislice pulse sequence, cine MRI was performed combined with cardiac gating. At the level of the pulmonary artery, the CT, spin echo (SE) MRI, and cine MRI findings were evaluated blindly by three groups of radiologists to determine whether the pulmonary artery was infiltrated or dynamically stenotic. Cine MRI allowed an overall diagnostic accuracy of 100% in comparison with SE MRI (91.6%) and CT (91.6%).

[Cine-MR in the assessment of the cardiovascular structures in extensive mediastinal pathology]. / La cine-RM nella valutazione delle strutture cardiovascolari nella patologia espansiva mediastinica.

The authors report their experience with cine-MRI in evaluating the infiltration of the main cardiovascular structures by expansive mediastinal masses. Twenty-four patients with proven lung (12 cases) and mediastinal (12 cases) cancers, previously selected with CT, underwent MRI examination of the chest. A superconductive 1.0 T unit was employed. Cytohistologic confirmation was obtained in all cases (6 thymomas, 6 lymphomas, 12 lung cancers). Ten normal subjects were also studied as a control group. Axial, coronal, and sagittal SE T1 images were obtained as the basis for MRI investigation of the chest. Subsequently, gradient-echo pulse-sequences images were obtained during the whole cardiac cycle, and later displayed on cine-Mode, on the section plane where vessel wall was closest to the tumor. The results prove cine-MRI to allow a correct diagnosis to be made in all cases, especially in those patients where CT and conventional SE MRI yielded questionable results. This preliminary experience suggests that cine-MRI could be used as a complementary method to CT and conventional SE MRI in evaluating mediastinal vessel involvement.

[Magnetic resonance in the tunnel carpal syndrome. Possibilities and perspectives of an etiopathogenetic study]. / La risonanza magnetica nella sindrome del tunnel carpale. Possibilità e prospettive di studio etiopatogenetico.

Thirty-four selected patients were evaluated in order to define MRI capabilities in the preoperative evaluation and characterization of the pathogenetic patterns of carpal tunnel syndrome (CTS). MRI examinations were performed by means of a superconductive unit (1.0 T, Magnetom): SE T1 (500/17) and T2 (2000/90) axial images of the carpal region were obtained with a round surface coil. In 8 patients 3D GE (FLASH) pulse sequences were used to obtain 32 images of the hand; 3D reconstruction was also applied. Six patients with rheumatoid arthritis and amyloidosis were also studied after i.v. injection of Gd-DTPA (0.2 mM/kg). MRI findings were compared with both clinico-electrophysiologic and surgical results. High agreement was observed only between MRI and surgical findings. MRI allowed the direct demonstration of carpal tunnel abnormalities in 8 cases, while abnormal findings in the median nerve were observed in 18 patients. The possibility of depicting medial nerve lesions on T2-weighted images when no direct demonstration of the cause of compression is possible, could represent a guideline for the etiopathogenetic investigation of CTS. However, further experience in selected patients is necessary to define all the aspects relative to this very common syndrome.

[Magnetic resonance of the rheumatoid hand]. / La risonanza Magnetica nella mano reumatoide.

The purpose of this study was to evaluate MRI diagnostic accuracy in rheumatoid arthritis (RA), to compare MRI and radiological findings and to correlate these findings with the clinical and serological profile of the disease. The hands of 24 patients (20 females, 4 males) affected with typical RA (ARA criteria) were studied using a tomograph Magnetom 1.0 T Siemens. Two patients affected with RA refractory to conventional second-line drugs who received a bolus of methylprednisolone (1 g) were studied before and after such treatment. The hands of healthy volunteers were examined as controls. Besides MRI study all patients underwent: (1) radiological examination of the hands performed with a standard technique and (2) clinical and serological investigation aimed at characterizing diseases activity and extent. The radiographic and MRI findings were evaluated by two different observers who found 15 pathological elementary lesions and assigned a MRI and a radiological score to each patient. MRI exhibited significantly higher accuracy than radiography in evaluating rheumatoid soft-tissue changes and in detecting minimal skeletal lesions, while severe skeletal lesions were better detected by radiology. No correlation was found between pathological MRI findings, radiological results and clinical or serological data. A significant drop in soft-tissue effusion was observed after methylprednisolone pulse in two patients. This study confirms MRI potential in the study of rheumatoid joint lesions and in the early detection of minimal soft-tissue changes. Its use appears to be suitable for accurate monitoring of RA patients under specific therapy.