Microbial bioprocess performance in nanoparticle-mediated composting.

Microbial composting is one of the most cost-effective techniques for degradation, remediation, nutrition, etc. Currently, there is faster growth and development in nanotechnology in different sectors. This development leads nanoparticles (NPs) to enter into the composts in different ways. First, unintentional entry of NPs into the composts via: waste discharge, buried solid waste, surface runoff, direct disposal into wastes (consumer goods, food, pharmaceuticals, and personal care products). Second, intentional mediation of the NPs in the composting process is a novel approach developed to enhance the degradation rate of wastes and as a nutrient for plants. The presence of NPs in the composts can cause nanotoxicity. Conversely, their presence might also be beneficial, such as soil reclamations, degradation, etc. Alternatively, metal NPs are also helpful for all living organisms, including microorganisms, in various biological processes, such as DNA replication, precursor biosynthesis, respiration, oxidative stress responses, and transcription. NPs show exemplary performance in multiple fields, whereas their role in composting process is worth studying. Consequently, this article aids the understanding of the role of NPs in the composting process and how far their presence can be beneficial. This article reviews the significance of NPs in: the composting process, microbial bioprocess performance during nano composting, basic life cycle assessment (LCA) of NP-mediated composting, and mode of action of the NPs in the soil matrix. This article also sheds insight on the notion of nanozymes and highlights their biocatalytic characterization, which will be helpful in future composting research.

Cow dung extract mediated green synthesis of zinc oxide nanoparticles for agricultural applications.

In the present study, zinc oxide nanoparticles (ZnO) were synthesized using cow dung extract to apply sustainable agriculture from rural resources. Studies on their antibacterial potential against E. coli DH 5 alpha indicated lower antimicrobial activities than the bulk Zn and commercial Zn nanoparticles. Compared with control and commercial ZnO nanoparticles, the maximum seed germination, root length, and shoot length were observed after the priming of synthesized ZnO NPs. This study suggests that ZnO may significantly increase seed germination and have lower antimicrobial potential. Further, the lower in-vitro cellular leakage and reactive oxygen species (ROS) production provided new hope for using cow dung extract mediated nanoparticles for agricultural and industrial applications.

Leveraging multiomics approaches for producing lignocellulose degrading enzymes.

Lignocellulosic materials form the building block of 50% of plant biomass comprising non-chewable agri-components like wheat straw, rice stubbles, wood shavings and other crop residues. The degradation of lignin, cellulose and hemicellulose is complicated and presently being done by chemical process for industrial application through a very energy intensive process. Lignin degradation is primarily an oxidative process where the enzyme lignin peroxidase digests the polymer into smaller fragments. Being a recalcitrant component, higher lignin content poses a challenge of lower recovery of product for industrial use. Globally, the scientists are working on leveraging fungal biotechnology for using the lignocellulose degrading enzymes secreted by actinomycetes and basidiomycetes fungal groups. Enzymes contributing to degradation of lignin are mainly performing the function of modifying the lignin and degrading the lignin. Ligninolytic enzymes do not act as an independent reaction but are vital to complete the degradation process. Microbial enzyme technology is an emerging green tool in industrial biotechnology for commercial application. Bioprocessing of lignocellulosic biomass is challenged by limitations in enzymatic and conversion process where pretreatment and separation steps are done to remove lignin and hydrolyze carbohydrate into fermentable sugars. This review highlights recent advances in molecular biotechnology, lignin valorization, sequencing, decipher microbial membership, and characterize enzyme diversity through 'omics' techniques. Emerging techniques to characterize the interwoven metabolism and spatial interactions between anaerobes are also reviewed, which will prove critical to developing a predictive understanding of anaerobic communities to guide in microbiome engineering This requires more synergistic collaborations from microbial biotechnologists, bioprocess engineers, enzymologists, and other biotechnological fields.

Phytomelatonin in stress management in agriculture.

Melatonin was discovered as a pineal gland hormone in animals and is now more significantly known as a signaling molecule in plants' biotic and abiotic stressors. Melatonin has been traced back to prokaryotic organisms during evolution and its primary function of antioxidant scavenging free radicals in photosynthetic prokaryotic bacteria is a lesser explored and exciting area for further research globally. The authors at IIT Delhi are trying to establish its potential role in stress management in agriculture. The present manuscript addresses the biosynthetic pathways hitherto suggested by scientists. In this manuscript, the potential scope of melatonin in agriculture as a growth promoter, post-harvest loss inhibitor, and signaling and quality improvement molecule is envisaged.

Prospects, challenges and need for regulation of nanotechnology with special reference to India.

To meet the challenges of food security in a sustainable way, technological intervention in agriculture is of prime importance. With the existing conventional technologies supporting high yield, better crop production and protection, the emphasis is now on use of nanotechnology. Exploring new applications of nanotechnology has gained momentum in recent years and has shown acceptance in sectors like food, medicine, chemistry etc. As compared to other sectors, the accepted role in agriculture can still be considered on the marginal side in terms of nanotechnology application. Nanotechnology offers a potential to open large scope of novel application in the fields of biotechnology and agricultural industries due to unique physicochemical properties. Nanoparticles can be used in herbicides, nano-pesticide, nano-fertilizers, or genes, which target specific cellular organelles in plant to release their content. It can potentially play an instrumental role in minimizing the application losses of agrochemicals due to their more stable emulsion, higher coverage on leaf surface, precision application, etc. Understanding that the conventional and macro-technologies in agriculture do not ensure sustainable food security and environmentally safe methods; the researchers are now inclined for more précised nanotechnology based applications. There is still a huge untapped potential of nanomaterials to be used a future technology in agriculture which remains to be explored. Besides offering potential benefits, there are high concerns about the potential challenges associated with nanoparticles. To address the safety concerns about nanoparticles, it is important that a strong regulation system should be developed. Presently, very few countries have started focusing on keeping nanoregulation on their agenda. It is very important for agricultural and biodiverse country like India to set up norms of use nanoregulation and bring nanotechnology on the safe technology net.