Transcription pattern of key molecular chaperones in heat shocked caprine cardiac fibroblasts.

The present study was attempted to unveil the impact of heat stress on transcription pattern of major heat shock response genes in caprine cardiac fibroblasts. Cardiac tissues (n = 6) were collected and primary cardiac cell culture was done. Cultured cardiac fibroblasts were kept in an atmosphere of 5% CO2 and 95% air at 38.5 °C. Cardiac cells achieved 70-75% confluence after 72 hours of incubation. Heat stress was induced on confluent cardiac fibroblasts at 42 °C for 0 (control), 20, 60, 100 and 200 min. Quantitative RT-PCR for ß2m (internal control), HSP60, HSP70, HSP90, and HSP110 was done and their transcription pattern was assessed by Pfaffl method. HSP60, HSP90, and HSP110 transcription did not differ at 20 min, up-regulated (p < 0.05) from 60 to 200 min and registered highest at 200 min of heat exposure. HSP70 transcription was gradually escalated (p < 0.05) time dependently from 20 to 200 min and reached zenith at 200 min of heat exposure. Differential induction in transcription of key molecular chaperones at various durations of heat exposure might reduce cardiac fibroblasts apoptosis and thus could maintain cardiac tissue function during heat stress.

Hyper-transcription of heat shock factors and heat shock proteins safeguard caprine cardiac cells against heat stress.

The present study was undertaken to document the transcriptional abundance of heat shock factors and heat shock proteins and their role in survivability of caprine cardiac cells during heat stress. Cardiac tissues were collected from different goats (n = 6) and primary cardiac cell culture was done in an atmosphere of 5% CO2 and 95% air at 38.5 °C. Cardiac cells accomplished 70-75% confluence after 72 h of incubation. Confluent cardiac cells were exposed to heat stress at 42 °C for 0 (control), 20, 60, 100 and 200 min. Quantitative RT-PCR for ß2m (internal control), heat shock factors (HSF1, HSF2, HSF4, HSF5), heat shock proteins (HSP10, HSP40), and Caspase-3 was done and their transcriptional abundance was assessed by Pfaffl method. Transcriptional abundance of HSF1, HSF2, and HSF4 did not change at 20 min, increased (P < 0.05) from 60 to 200 min and reached zenith at 200 min of heat exposure. However, transcriptional abundance of HSF5 was gradually escalated (P < 0.05) from 20 to 200 min and registered highest at 200 min of heat exposure. Transcriptional abundance of HSP10 and HSP40 followed an similar pattern like that of HSF5. Transcriptional abundance of Caspase-3 was significantly down-regulated at 200 min of heat exposure. It could be speculated that over-expression of HSFs and HSPs might have reduced Caspase-3 expression at 200 min of heat exposure suggesting their involvement in cardiac cells survival under heat stress. Moreover, hyper-expression of HSFs and HSPs could maintain the integrity and endurance of cardiac tissues of goats under heat stress.

A New Numerical Approach for the Analysis of Variable Fractal and Fractional Order Differential Equations.

The variable fractional dimensions differential and integral operator overrides the phenomenon of the constant fractional order. This leads to exploring some new ideas in the proposed direction due to its varied applications in the recent era of science and engineering. The present papers deal with the replacement of the constant fractional order by variable fractional order in various fractal-fractional differential equations. An advanced numerical scheme is developed with the help of Lagrange three-point interpolation and further, it is employed for the solution of the proposed differential equations. However, the properties of these new operators are presented in detail. Finally, the error analysis is also conducted for the numerical scheme deployed. The results are validated by the suitable choice of applications to real-life problems. The well- known multi-step-Adams-Bashforth numerical scheme for classical differential equations is recovered when the non-integer order is one.

Thalidomide and steroid in the management of erythema nodosum leprosum.

OBJECTIVE: The objective of the study was to assess the efficacy and safety profiles of combined treatment of prednisolone with thalidomide (Gr-A) and prednisolone with clofazimine (Gr. B) in patients with erythema nodosum leprosum (ENL) or type 2 lepra reactions. MATERIALS AND METHODS: Efficacy of both regimens was assessed on the basis of clinical recovery of recurrent ENL measured by reaction severity score (RSS), Visual Analog Scale (VAS), and recurrence of type 2 lepra reaction. The causality assessment of adverse drug reactions was done using the WHO UMC causality assessment scale. RESULTS: The average age of patients with recurrent ENL was 42.8 years (male) and 51.8yrs (female) and had mean duration of leprosy and recurrent ENL 2.4 years and 2.09 years, respectively. 80% of nonrecurrence was observed in Gr-A versus 66% in Gr-B. Significant (P < 0.05) lower RSS and VAS was found in both the treatment groups as compared to pretreatment value. The reduction in RSS and VAS was statistically significant (P < 0.05) in Gr-A compared to Gr-B treatment. CONCLUSION: Thalidomide combination with steroid was found to be more efficacious than clofazimine combination with steroid in the treatment of ENL both the treatment regimens showed few tolerable side effects. Improved strategies for the treatment and management of these reactions need to be developed.

A National Equity Initiative to Address Noncommunicable Diseases and Injuries: Findings and Recommendation from the Nepal NCDI Poverty Commission.

We aimed to assess the burden of NCDIs across socioeconomic groups, their economic impact, existing health service readiness and availability, current policy frameworks and national investment, and planned programmatic initiatives in Nepal through a comprehensive literature review. Secondary data from Global Burden of Disease estimates from GBD 2015 and National Living Standard Survey 2011 were used to estimate the burden of NCDI and present the relationship of NCDI burden with socioeconomic status. The Commission used these data to define priority NCDI conditions and recommend potential cost-effective, poverty-averting, and equity-promoting health system interventions. NCDIs disproportionately affect the health and well-being of poorer populations in Nepal and cause significant impoverishment. The Commission found a high diversity of NCDIs in Nepal, with approximately 60% of the morbidity and mortality caused by NCDIs without primary quantified behavioral or metabolic risk factors, and nearly half of all NCDI-related DALYs occurring in Nepalese younger than 40 years. The Commission prioritized an expanded set of twenty-five NCDI conditions and recommended introduction or scale-up of twenty-three evidence-based health sector interventions. Implementation of these interventions would avert an estimated 9680 premature deaths per annum by 2030 and would cost approximately $8.76 per capita. The Commission modelled potential financing mechanisms, including increased excise taxation on tobacco, alcohol, and sugar-sweetened beverages, which would provide significant revenue for NCDI-related expenditures. Overall, the Commission's conclusions are expected to be a valuable contribution to equitable NCDI planning in Nepal and similar resource-constrained settings globally.

Significance of molecular chaperones and micro RNAs in acquisition of thermo-tolerance in dairy cattle.

Ambient temperature is considered as the major abiotic factor which regulates body physiological mechanisms of all living creatures across the globe. Variation in ambient temperature which emulates thermoneutral zone culminates in heat stress. Heat stress has been emerged as major ultimatum to livestock's growth, development, production and reproduction across the world. Livestock's responds to the heat stress via different mechanisms such as behavioral, physiological, biochemical, endocrine and molecular mechanisms. Amongst the aforementioned mechanisms, molecular mechanism plays crucial role to achieve thermo-tolerance via expression of highly conserved family of proteins known as heat shock proteins (HSPs) across livestock species. HSPs serve as molecular chaperones to ameliorate the menace of heat stress in domestic species. In addition, microRNAs are small non-coding RNA which down regulates post-transcriptional gene expression by targeting various HSPs to regulate the thermoregulatory responses in livestock species. Despite of thermal adaptation mechanisms, heat stress breaches animal body homeostasis thereby depresses their production and productivity. Therefore, veterinary researches have been targeting to explore different repertoire of HSPs and microRNAs expression to counteract the rigors of heat stress thereby confer thermo-tolerance in livestock species. The present review highlights the significance of molecular chaperones and microRNAs in the acquisition of thermo-tolerance in dairy cattle.

Exchange bias and Verwey transition in Fe5C2/Fe3O4 core/shell nanoparticles.

This report presents new findings of exchange bias and related structural and magnetic properties in iron carbide/magnetite (Fe5C2/Fe3O4) core/shell nanoparticles. The exchange bias emerges from an energetic landscape, namely a first-order phase transition-the Verwey transition at 125 K, during which the Fe3O4 shell changes from the cubic to monoclinic structure. The phase transition leads to the exchange bias because it results in abrupt changes in magnetocrystalline anisotropy and exchange coupling. Another unique phenomenon identified in this composite system is enhanced magnetic coercivity due to the uniaxial anisotropy of the monoclinic phase. An analysis of the correlations between the observed phenomena is given based on the temperature dependence of the coercivity, the exchange bias field values, and the Verwey transition temperature.

Behavioural, physiological, neuro-endocrine and molecular responses of cattle against heat stress: an updated review.

The negative impact of heat stress on cattle growth, development, reproduction and production has been quite alarming across the world. Climate change elevates earth surface temperature which exacerbates the wrath of heat stress on cattle. Moreover, cattle in tropical and sub-tropical countries are most commonly affected by the menace of heat stress which severely wane their production and productivity. In general, cattle exhibit various thermoregulatory responses such as behavioural, physiological, neuro-endocrine and molecular responses to counteract the terrible effects of heat stress. Amongst the aforementioned thermoregulatory responses, behavioural, physiological and neuro-endocrine responses are regarded as most conventional and expeditious responses shown by cattle against heat stress. Furthermore, molecular responses serve as the major adaptive response to attenuate the harmful effects of heat stress. Therefore, present review highlights the significance of behavioural, physiological, neuro-endocrine and molecular responses which act synergistically to combat the deleterious effects of heat stress thereby confer thermo-tolerance in cattle.

Thermoregulatory responses in riverine buffaloes against heat stress: An updated review.

High heat and humidity stress have been a perpetual perilous for the buffalo's production and productivity in tropics and subtropics including India. Productive potential of livestock's species including buffaloes is maximum with in thermo-neutral zone (TNZ) and if ambient temperature exceeds TNZ and upper critical temperature expose livestock's to heat stress conditions. For decades, heat stress has been the prime factor to plummet buffalo's growth, development, reproduction and production in tropics and subtropics including India. In general, buffaloes are homeotherms and known as temperature regulators as they resist the variations in ambient temperatures. Generally, buffaloes like other livestock's display amalgamation of thermoregulatory responses to withstand the changes occurred in their micro and macro environment. These thermoregulatory responses are behavioural, physiological, neuro-endocrine and molecular responses acting synergistically to counteract the deleterious effects of heat stress. Amidst all responses, molecular responses play major role to confer thermo-tolerance through expression of highly conserved family of proteins known as heat shock proteins (HSPs). Despite of these thermoregulatory responses, heat stress prodigiously muddles buffalo's production and productivity. The present review highlights the thermoregulatory responses manifested by riverine buffaloes against heat stress.

Duration of estrogen exposure during reproductive years, age at menarche and age at menopause, and risk of cardiovascular disease events, all-cause and cardiovascular mortality: a systematic review and meta-analysis.

BACKGROUND: Little is known about the estrogen exposure measurement and mutual effect of age at menarche and age at menopause in the risk of cardiovascular disease (CVD) events. OBJECTIVES: To evaluate estrogen exposure measurement and describe mutual effect of age at menarche and age at menopause in the risk of CVD events. SEARCH STRATEGY: Systematic review of literature in PubMed, Embase and Web of Science for studies published up to 28 June 2020. SELECTION CRITERIA: Observational studies related to estrogen exposure measurement, including mutual effect of age at menarche and age at menopause and risk of CVD events. DATA COLLECTION AND ANALYSIS: Synthesis of evidence was conducted by reviewing individual estimates, followed by meta-analysis. The study received no external funding. MAIN RESULTS: A total of 75 studies were included in synthesis of evidence, of which 17 studies were included in meta-analysis. Reproductive lifespan (age at menopause - age at menarche), endogenous estrogen exposure and total estrogen exposure were used for estrogen exposure measurement. Reproductive lifespan was by far the most commonly used method for estrogen exposure measurement. A shorter reproductive lifespan was associated with a higher risk of CVD events; the pooled relative risk (95% CI) was 1.31 (1.25-1.36) for stroke events. Robust epidemiological studies with measurement of estrogen exposure and associated health risk would strengthen the evidence. CONCLUSIONS: Reproductive lifespan was the most commonly used method for estrogen exposure measurement in epidemiological studies. A shorter reproductive lifespan was associated with a higher risk of CVD events, particularly stroke. TWEETABLE ABSTRACT: A systematic review and meta-analysis found that women with a shorter reproductive lifespan have a higher risk of stroke events.

Impact of thermal stress on expression dynamics of HSP60 in cardiac fibroblast cells of goat.

The present study was aimed to determine the impact of thermal stress on expression dynamics of heat shock protein 60 (HSP60) mRNA in cultured cardiac fibroblast cells of the goat. The heart tissues (n = 6) from different goats were used for the culture study. The cardiac fibroblast cells were cultured and subjected to thermal stress at 42 °C for 0, 20, 60 and 100 min. The relative abundance of HSP60 mRNA was assessed by quantitative real-time PCR (qRT-PCR). The cardiac cells exposed to thermal stress at 42 °C for 0 min was taken as control. The relative abundance of HSP60 mRNA did not change at 20 min of thermal stress as compared to control. Thereafter, the relative abundance of HSP60 mRNA was significantly up-regulated (p < 0.05) at 60 min and 100 min of thermal stress. However, the highest mRNA expression of HSP60 was noticed at 100 min of thermal stress. The present study indicates that, thermal stress modulates the mRNA expression HSP60 in cultured caprine cardiac fibroblast cells.

Novel Molten Salt Assisted Autocombustion Method for the Synthesis of Aluminum-Doped SrFe12-xAlxO19 Hexaferrite Nanoparticles.

The study presents a novel molten salt assisted autocombustion synthesis of SrFe12-xAlxO19 particles. The extrinsic magnetic properties such as coercivity and the remanence of sintered M-type ferrites are highly dependent on the microstructure viz. morphology and size, of the ferrite particles. The control of the microstructures of ferrite particles is usually achieved via control nucleation and grain growth process. In this study, NaCl salt was used to control the crystal shape and size of Al3+ doped SrFe12-xAlxO19 particles. The presented novel method couples advantage of deriving homogenized particles via auctocombustion first and later sintering in the presence of NaCl salt. Highly dispersed, homogeneous, and hexagonal shaped SrFe12-xAlxO19 ferrite particles were achieved with this method. The particles derived via the molten salt assisted method presented a high coercivity and squareness ratio (>0.5) as compared to that obtained via autocombustion method only.

Impact of heat stress on transcriptional abundance of HSP70 in cardiac cells of goat.

The present study was aimed to document the effect of heat stress on the transcriptional abundance of heat shock protein 70 (HSP70) mRNA in cultured cardiac cells of goat. The heart tissues (n = 6) from different goats were used for the culture study. The cardiac cells obtained from different heart tissues were cultured in 24 well cell culture plates and incubated in a humidified CO2 (5%) incubator at 37 °C. The cardiac cells were allowed to become 75-80% confluent after 72 h of incubation. Thereafter, the cardiac cells were subjected to heat exposure at 42 °C (heat exposed) for 0, 20, 60 and 100 min. The cardiac cells exposed to heat stress at 42 °C for 0 min was taken as control. The relative abundance of HSP70 mRNA was gradually up-regulated (p < .05) from 20 to 100 min of heat exposure and reached the zenith (p < .05) at 100 min of heat challenge. The present finding highlights that, HSP70 could possibly act as a cytoprotective factor and may promote cardiac cell survival against the detrimental effect of heat stress. Moreover, this study may serve as the harbinger to conduct further research work on expression kinetics of HSP70 in cardiac cells of goat including other livestock species.

Influence of Urea on the Synthesis of NiCo2O4 Nanostructure: Morphological and Electrochemical Studies.

The widespread use of miniature electronic devices calls for energy-dense storage strategies. The supercapacitor-based energy storage devices with high areal capacitance are desired energy storage alternative. It is still a challenge to fabricate supercapacitor-based energy devices with consistent performance. The porous metal oxides with large areal capacitance are desired materials for electrode, but there exists a limited understanding of the influence of synthesis parameters on microstructural properties, which largely govern their electrochemical performance. In the present work, hierarchal spinel nickel cobaltite (NiCo2O4) nanostructures were synthesized in the presence of the varying amount of hydrolyzing agent via a simple hydrothermal method coupled with a simple post-annealing process. This work focuses on understanding the influence of hydrolyzing agent in controlling the microstructure and hence ensuing electrochemical properties of the NiCo2O4 based electrode. Based on the urea hydrolyzing content, the as synthesized NiCo2O4 nanostructure varied from the rod, plate to nanoflower. The mesoporous nanostructures, with urea content 1.49 gm, exhibit a sizeable BJH surface area (79.2 m² g-1) and high mesopore volume (0.140 cm³ g-1). Remarkably, the NiCo2O4 nanoflower shows high specific capacitance of 3143.451 F/g at 2 mV/s scan rate, 1264.5 F/g at 1 A/g current density, energy density of 56 Wh/kg and power density of 8,400 W/kg in 3 M KOH electrolyte. The capacitance loss after 5000 cycles is 48% at the current density of 10 A/g, indicating their excellent cycling stability. The impressive electrocatalytic activity is largely ascribed to the high intrinsic electronic conductivity, superior mesoporous nanostructures and rich surface Ni active species of the NiCo2O4 materials, which can largely boost the interfacial electroactive sites and charge transfer rates indicating promising applications as electrodes in future supercapacitors.

Facile Synthesis of Bio-Templated Tubular Co3O4 Microstructure and Its Electrochemical Performance in Aqueous Electrolytes.

Template-assisted facile synthesis of tubular Co3O4 microstructures and its electrochemical performance was studied to understand its use as a potential electrode material for supercapacitors. Tubular porous Co3O4 microstructures were synthesized using cotton fibers as bio-template. The as-obtained templated Co3O4 structure inherits the morphology and microstructure of cotton fiber. The electrochemical performance of the electrode made up of tubular Co3O4 structure was evaluated in 3 M KOH, NaOH, and LiOH aqueous electrolytes. The large-surface-area of tubular Co3O4 microstructure has a noticeable pseudocapacitive performance with a capacitance of 401 F/g at 1 A/g and 828 F/g at 2 mV/s, a Coulombic efficiency averaging ~100%, and excellent cycling stability with capacitance retention of about 80% after 5,000 cycles. Overall, the tubular Co3O4 microstructure displayed superior electrochemical performance in 3 M KOH electrolyte with peak power density reaching 5,500 W/kg and energy density exceeding 22 Wh/kg. The superior performance of tubular Co3O4 microstructure electrode is attributed to its high surface area and adequate pore volume distribution, which allows effective redox reaction and diffusion of hydrated ions. The facile synthesis method can be adapted for preparing various metal oxide microstructures for possible applications in catalysis, electrochemical, sensors, and fuel cells applications.

Physical Properties and Theoretical Study of NixCo3-xO4 (0 ≤ x ≤ 1.5) Nanostructures as High-Performance Electrode Materials for Supercapacitors.

Study evaluates the electrochemical performance of off-stoichiometric NixCo3-xO4 compounds. The off-stoichiometric samples were prepared via hydrothermal technique by systematically varying Co/Ni molar ratio. Physical and electrochemical properties of NixCo3-xO4 were observed to be stoichiometry dependent. The increase in Ni/Co ratio in NixCo3-xO4 leads to the morphological transformation from fibrous bundles to urchin like nanospheres with a concomitant increase in the surface area reaching up to 132 m2/g. The optimal specific capacitance of 225 F/g at a current density of 1 A/g and 524 F/g at 10 mV/s scan rate was observed of x 1.0 sample, with an increased retention capacity ∼120% measured at 2 A/g current density. The hybrid density functional theory (DFT) calculations of the electronic density of states identified Ni1.0Co2O4 with optimal band-gap of 2.38 eV with an expectation of displaying higher electrocapacitive performance. Experimentally, Ni0.92Co2.08O4 displayed superior electrocapacitive performance among all Ni/Co ratio in NixCo3-xO4. The DFT study also predicted Ni preference to the octahedral site, which is in-line with the observed increase in ferromagnetic nature, decreased lattice parameter, and increased structural disorder with increasing Ni/Co ratio. The improved electrochemical performance of NixCo3-xO4 (x > 0) is attributed to the mesoporous hierarchical structure, with a high electroactive surface which can effectively improve structural stability, and reduce the ionic and electron diffusion length. Compared to the pure Co3O4, the reduction of Co content in NixCo3-xO4 is desired due to the high cost and toxicity of Co element.

Magnetic Stimulus Responsive DDS Based on Chitosan Microbeads Embedded with Magnetic Nanoparticles.

In this paper, we have presented a novel Drug Delivery Substrate (DDS) that that is responsive to external stimuli of high-frequency alternating magnetic fields. The DDS is constituted of chitosan crosslinked with PEGDMA (polyethylene glycol dimethacrylate), loaded with Fe3O4 magnetic nanoparticles and vancomycin. In another experiment, a 19-hour elution was observed where three magnetic stimuli of 25 mT, 109.9 kHz were given for 60 min to the test samples. The stimuli were separated by several hours. After excitation span, it was observed that the stimulated samples released a significantly higher amount of vancomycin by as much as 21% compared to non-stimulated samples. In another study, preliminary results showing the effect of different PEGDMA chain lengths have been discussed. These results show evidence of a smart, controllable DDS that allows modulation of its normal passive antibiotic elution by applying external stimuli per personalized needs.

Comparative therapeutic efficacy of levofloxacin, ornidazole and alpha tocopherol combination with prostaglandin F2α on IL-6 and IL-10 transcript level in longstanding cases of endometritis in crossbreed Jersey cows.

This study compared the therapeutic efficacy of levofloxacin, ornidazole and alpha tocopherol combination and prostaglandin F2α (PGF2α) in longstanding cases of endometritis and evaluated their impact on Interleukin-6 (IL-6) and Interleukin-10 (IL-10) transcript level in peripheral blood leukocytes. Eighteen endometritic crossbred Jersey cows were randomly allotted to three groups (six in each) viz. Group I (levofloxacin combo treatment I/U), group II (PGF2α treatment I/M), group III (no treatment, control), and group IV (six non-endometritic healthy cyclic) was taken for comparison study. The clinical efficacy was assessed by haematological study (TLC: Total leukocyte count; DC: Differential count), polymorphonuclear leukocytes (PMN) count in uterine cytology and relative mRNA expression of IL-6 and IL-10 in peripheral blood leukocytes before and after treatment with respect to conception rate following single and second inseminations. Group I and II registered significant increase in TLC and neutrophil count. PMN cytology was increased two and three fold in group I and II, respectively. The IL-6 transcript level was increased by 2.5 and 4.6 fold while that of IL-10 increased by 3.7 and 5.2 fold in group I and II, respectively. Conception rate across group I to IV following single insemination was found to be 66.67%, 50%, 16.67%, and 83.33% and their corresponding values following second insemination were 66.67%, 83.33%, 16.67%, and 83.33%, respectively. Thus, the administration of levofloxacin combo and PGF2α might have better conception rate following first and second insemination, respectively. Our study also reveals that PGF2α could register better clearance of bacteria through stronger PMN cell and cytokine activity in post-treatment period.

Transcriptional and translational abundance of Bone morphogenetic protein (BMP) 2, 4, 6, 7 and their receptors BMPR1A, 1B and BMPR2 in buffalo ovarian follicle and the role of BMP4 and BMP7 on estrogen production and survival of cultured granulosa cells.

BMPs and their receptors modulate the granulosa cell (GC) function in the follicle of domestic animals. Since little is known on BMPs in the buffalo, the present study was aimed to investigate the expression of BMP2, 4, 6, 7 and their receptors BMPR1A, BMPR1B, BMPR2 in the GC and theca cells (TC) of ovarian follicles and the role of BMP4 and BMP7 on buffalo GC. Follicles were classified into four groups based on size and E2 level in the follicular fluid as follows: (i) Group1(4-6 mm; <0.5 ng/mL) (ii) Group 2 (7-9 mm; 0.5-5 ng/mL) (iii) Group 3 (10-13 mm; 5-40 ng/mL) and (iv) Group 4 (dominant follicle) (>13 mm; >180 ng/mL). The results revealed that except BMP6, BMP2, 4 7 and receptors BMPR1A, BMPR1B and BMPR2 showed a minimum of 1.5-2 fold increase in mRNA expression in the GC of dominant follicle as compared to other follicle classes. In the dominant follicle, a two-fold increase in BMP4 and BMP7 expression was observed in the TC. At 100 ng/mL, the BMP4 and BMP7 either alone or in combination maximally down-regulated CASPASE3 and stimulated the transcripts of PCNA, FSHR and CYP19A1 that was supported by E2 secretion in the granulosa cell culture suggesting their role in cell survival and E2 production. In conclusion, GC and TC of dominant follicles express BMP 2, 4, 6, 7 and their receptors BMPR1A, BMPR1B and BMPR2. BMP4 and BMP7 stimulate E2 production and promote GC survival.

Development and evaluation of an oral fast disintegrating anti-allergic film using hot-melt extrusion technology.

The main objective of this novel study was to develop chlorpheniramine maleate orally disintegrating films (ODF) using hot-melt extrusion technology and evaluate the characteristics of the formulation using in vitro and in vivo methods. Modified starch with glycerol was used as a polymer matrix for melt extrusion. Sweetening and saliva-simulating agents were incorporated to improve palatability and lower the disintegration time of film formulations. A standard screw configuration was applied, and the last zone of the barrel was opened to discharge water vapors, which helped to manufacture non-sticky, clear, and uniform films. The film formulations demonstrated rapid disintegration times (6-11s) and more than 95% dissolution in 5min. In addition, the films had characteristic mechanical properties that were helpful in handling and storage. An animal model was employed to determine the taste masking of melt-extruded films. The lead film formulation was subjected to a human panel for evaluation of extent of taste masking and disintegration.