RESUMO
A seroepidemiological survey of Neospora caninum infection among dogs in Japan was conducted using species-specific enzyme-linked immunosorbent assay with recombinant surface antigen (Nc-SAG1t). Among 1,206 dogs examined, 126 dogs (10.4%) from 30 prefectures from Hokkaido to Okinawa were positive to N. caninum infections, which were more frequently detected in females than males. Siberian Huskies showed the highest positive rate compared with the other breeds. Dogs with pyometra and diabetes mellitus showed the higher positive rates than dogs with other diseases or without diseases.
Assuntos
Anticorpos Antiprotozoários/sangue , Coccidiose/veterinária , Doenças do Cão/epidemiologia , Neospora/imunologia , Envelhecimento , Animais , Coccidiose/epidemiologia , Doenças do Cão/parasitologia , Cães , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Japão/epidemiologia , Masculino , Estudos SoroepidemiológicosRESUMO
A nationwide epidemiological survey of Babesia gibsoni infection in non-fighting dogs was conducted using an improved ELISA with recombinant B. gibsoni thrombospondin-related adhesive protein (BgTRAP). A total of 1206 dogs from 27 prefectures were examined and 128 (10.6%) tested positive. In the eastern part of Japan, 39 dogs out of the 559 (7.0%) examined were positive, while 89 dogs out of 647 (13.8%) tested positive in the western part of Japan. Although the percentage of dogs that tested positive was significantly (p=0.0001) lower in the eastern part compared to the western part of Japan, overall these results indicate that B. gibsoni infection of dogs has a widespread geographic distribution throughout the country. A history of tick infestation was identified as a significant risk factor for B. gibsoni infection (p=0.0091), while sex (p=0.9411), age (p=0.0920) and breed (p=0.0549) of dogs were not statistically significant risk factors. These results indicate that tick infestation is the most dominant risk factor for B. gibsoni infection of non-fighting dogs in Japan and suggest that other B. gibsoni transmission routes, such as fighting and transplacental transmission, may be less important.
Assuntos
Antígenos de Protozoários/imunologia , Babesia/imunologia , Babesia/isolamento & purificação , Babesiose/veterinária , Doenças do Cão/epidemiologia , Doenças do Cão/parasitologia , Proteínas de Protozoários/imunologia , Animais , Anticorpos Antiprotozoários/sangue , Babesiose/epidemiologia , Babesiose/imunologia , Doenças do Cão/imunologia , Cães , Ensaio de Imunoadsorção Enzimática , Feminino , Geografia , Japão/epidemiologia , Masculino , Fatores de Risco , Infestações por Carrapato/veterináriaRESUMO
Antibodies against Rickettsia japonica in 20 of 1,207 dogs and 5 of 584 cats in Japan were detected using immunofluorescence. Some antibody-positive animals were detected in Niigata and Kagawa Prefectures, areas in which Japanese spotted fever in human patients has never been identified. Some animals were positive for antibodies against other new Rickettsia species.
