Co3O4 Nanostructured Sensor for Electrochemical Detection of H2O2 as a Stress Biomarker in Barley: Fe3O4 Nanoparticles-Mediated Enhancement of Salt Stress Tolerance.

This research investigates the enhancement of barley's resistance to salt stress by integrating nanoparticles and employing a nanostructured Co3O4 sensor for the electrochemical detection of hydrogen peroxide (H2O2), a crucial indicator of oxidative stress. The novel sensor, featuring petal-shaped Co3O4 nanostructures, exhibits remarkable precision and sensitivity to H2O2 in buffer solution, showcasing notable efficacy in complex analytes like plant juice. The research establishes that the introduction of Fe3O4 nanoparticles significantly improves barley's ability to withstand salt stress, leading to a reduction in detected H2O2 concentrations, alongside positive impacts on morphological parameters and photosynthesis rates. The developed sensor promises to provide real-time monitoring of barley stress responses, providing valuable information on increasing tolerance to crop stressors.

Glyphosate Sensor Based on Nanostructured Water-Gated CuO Field-Effect Transistor.

This research presents a comparative analysis of water-gated thin film transistors based on a copper oxide (CuO) semiconductor in the form of a smooth film and a nanostructured surface. A smooth CuO film was deposited through reactive magnetron sputtering followed by annealing in atmosphere at a temperature of 280 ∘C. Copper oxide nanostructures were obtained by hydrothermal synthesis on a preliminary magnetron sputtered 2 nm thick CuO precursor followed by annealing at 280 ∘C. An X-ray diffraction (XRD) analysis of the samples revealed the presence of a tenorite (CuO) phase with a predominant orientation of (002). Scanning electron microscopy (SEM) and atomic force microscopy (AFM) studies of the samples revealed a highly developed surface with crystallites having a monoclinic syngony and dimensions of 15-20 nm in thickness, 150 nm in length, and 100 nm in height relative to a 2.5 nm height for the CuO crystallites of the smooth film. Electric measurements of the studied devices revealed typical current-voltage characteristics of semiconductors with predominant hole conductivity. The maximum ON/OFF ratio at a rain-source voltage of 0.4 volts and -1.2 volts on the gate for a smooth film was 102, and for a nanostructured transistor, it was 103. However, a much stronger saturation of the channel was observed for the nanostructured channel than for the smooth film. A test solution containing glyphosate dissolved in deionized water in three different concentrations of 5, 10, and 15 µmol/L was used during the experiments. The principle of operation was based on the preliminary saturation of the solution with Cu ions, followed by the formation of a metal-organic complex alongside glyphate. The glyphosate contents in the analyte led to a decrease in the conductivity of the transistor on the axis of the smooth film. In turn, the opposite effect was observed on the nanostructured surface, i.e., an increase in conductivity was noted upon the introduction of an analyte. Despite this, the overall sensitivity of the nanostructured device was twice as high as that of the device with a thin film channel. The relative changes in the field-effect transistor (FET) conductivity at maximum glyphosate concentrations of 15 µmol/L reached 19.42% for the nanostructured CuO film and 3.3% for the smooth film.

A non-enzymatic electrochemical hydrogen peroxide sensor based on copper oxide nanostructures.

This article describes the synthesis of nanostructured copper oxide on copper wires and its application for the detection of hydrogen peroxide. Copper oxide petal nanostructures were obtained by a one-step hydrothermal oxidation method. The resulting coating is uniform and dense and shows good adhesion to the wire surface. Structure, surface, and composition of the obtained samples were studied using field-emission scanning electron microscopy along with energy-dispersive spectroscopy and X-ray diffractometry. The resulting nanostructured samples were used for electrochemical determination of the H2O2 content in a 0.1 M NaOH buffer solution using cyclic voltammetry, differential pulse voltammetry, and i-t measurements. A good linear relationship between the peak current and the concentration of H2O2 in the range from 10 to 1800 µM was obtained. The sensitivity of the obtained CuO electrode is 439.19 µA·mM-1. The calculated limit of detection is 1.34 µM, assuming a signal-to-noise ratio of 3. The investigation of the system for sensitivity to interference showed that the most common interfering substances, that is, ascorbic acid, uric acid, dopamine, NaCl, glucose, and acetaminophen, do not affect the electrochemical response. The real milk sample test showed a high recovery rate (more than 95%). According to the obtained results, this sensor is suitable for practical use for the qualitative detection of H2O2 in real samples, as well as for the quantitative determination of its concentration.