RESUMO
Angelica keiskei is a traditional herb peculiar to Japan and abundantly contains vitamins, dietary fiber and such polyphenols as chalcone. We investigated in the present study the effect of A. keiskei on insulin resistance and hypertriglyceridemia in fructose-drinking rats as a model for the metabolic syndrome. Male Wistar rats were given a 15% fructose solution as drinking water for 11 weeks. Fructose significantly increased the levels of serum insulin and triglyceride (TG) compared with the control level. Treatment with an ethanol extract of A. keiskei (AE) significantly reduced the levels of blood glucose (-16.5%), serum insulin (-47.3%), HOMA-R (-56.4%) and TG (-24.2%). A hepatic gene analysis showed that fructose reduced the expression of the genes related to fatty acid ß-oxidation and high-density lipoprotein (HDL) production. Treatment with AE enhanced the expression of the acyl-CoA oxidase 1 (ACO1), medium-chain acyl-CoA dehydrogenase (MCAD), ATP-binding membrane cassette transporter A1 (ABCA1) and apolipoprotein A1 (Apo-A1) genes. These results suggest that AE improved the insulin resistance and hypertriglyceridemia of the fructose-drinking rats.
Assuntos
Angelica/química , Hipertrigliceridemia/tratamento farmacológico , Hipolipemiantes/farmacologia , Resistência à Insulina , Síndrome Metabólica/tratamento farmacológico , Extratos Vegetais/farmacologia , Transportador 1 de Cassete de Ligação de ATP , Transportadores de Cassetes de Ligação de ATP/metabolismo , Acil-CoA Desidrogenase/metabolismo , Acil-CoA Oxidase/metabolismo , Animais , Apolipoproteína A-I/metabolismo , Glicemia/análise , Água Potável/administração & dosagem , Frutose/administração & dosagem , Expressão Gênica/efeitos dos fármacos , Hipertrigliceridemia/sangue , Hipertrigliceridemia/induzido quimicamente , Hipolipemiantes/isolamento & purificação , Insulina/sangue , Lipoproteínas HDL/sangue , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Síndrome Metabólica/sangue , Síndrome Metabólica/induzido quimicamente , Extratos Vegetais/química , Ratos , Ratos Wistar , Triglicerídeos/sangueRESUMO
The protective effects of immunization with Candida membrane antigen (CMA) on a systemic infection originating from intestinally colonized Candida albicans were examined. The colonization of orally inoculated C. albicans in the intestinal tract was established in BALB/c mice that had been concomitantly treated with oral doses of antibacterial drugs. In these animals, a systemic dissemination of C. albicans with fatal outcome was induced by a repeated dosing of prednisolone. In this endogenous infection model, the effects of immunization by CMA on the infection were examined. CMA-immunized mice showed a longer lifespan than unimmunized mice. The protective effect of CMA immunization in immunosuppressed mice was also measured by a decrease in body weight loss after treatment with prednisolone and in the number of viable Candida cells in the target organs, the kidneys and livers. However, the CFU of C. albicans in the intestinal tract was not significantly lowered. These results suggest that CMA immunization inhibited the dissemination of systemic Candida infection from the intestinal tract induced by treatment with prednisolone.
