Abundance of microplastics at and near a shellfish aquaculture farm: An eastern oyster (Crassostrea virginica) transplant study.

Microplastics (MP) have repeatedly been found in commercially cultured species of bivalves. There are concerns regarding the amount of MP released into the environment by aquaculture activities, and questions regarding possible higher MP loads in farm-grown shellfish compared to levels in shellfish collected from recreational beds. To explore this concept, seawater, aquaculture gear, and eastern oysters (Crassostrea virginica) were sampled from an aquaculture site in Niantic Bay, CT, USA, and a 2-week transplantation experiment was performed in which oysters were transplanted between the aquaculture site and a plastic-free cage off the dock at the University of Connecticut-Avery Point campus. The digestive gland-stomach complex (gut) was dissected from the oysters and MP were extracted from the adjacent seawater and oyster gut samples using previously validated extraction methods. Extensive quality assurance and control measures were taken to reduce MP contamination. Particles in all samples were isolated, imaged under a stereomicroscope, and characterized (size, shape, polymer) using ImageJ software and micro-Fourier transform infrared spectroscopy. Water samples contained 0-0.3 MP/L and oyster gut samples contained 0-1.3 MP/g wet weight indicating very low concentrations of MP at the farm (0-2 MP/individual) or away from the farm (0-3 MP/individual). Aquaculture gear in this area is not contributing to MP ingestion in farmed oysters or elevated MP levels in the surrounding water.

The relationship between microplastics in eastern oysters (Crassostrea virginica) and surrounding environmental compartments in Long Island Sound.

Microplastics (MP, <5 mm) are found in coastal waters across various environmental compartments (biota, water, marine snow, sediment). The eastern oyster (Crassostrea virginica) is a commercially important species that ingests MP; however, oysters are discriminant suspension feeders that do not consume all particles to which they are exposed. This study explored the relationship between MP in oysters on a recreational oyster bed and the surrounding environmental compartments in Long Island Sound (LIS; USA). The quantity and types of MP in oysters, water, marine snow, and sediment samples were determined. Precautions were taken to minimize and monitor MP contamination in the field and laboratory to improve the quality of data collected. Microplastics were isolated from samples via chemical digestion, and any suspected particles were identified using micro-Fourier transform infrared spectroscopy. A total of 86 MP were identified out of 885 suspected particles across environmental media. The highest MP count in an individual oyster was nine, indicating low concentrations of MP in oysters and the surrounding environment. Few polymers, except polyethylene terephthalate, were shared between oysters and the surrounding environmental compartments. Sediments contained the highest number of MP across all environmental compartments (42 total). These data aid in determining the types of MP (polymer composition, shape, size) to which oysters are exposed and identified those ingested. The low numbers of MP recorded, coupled with the lack of alignment of polymers between oysters and their surrounding environment, demonstrates further that oysters are a poor bioindicator species for MP pollution.

Determining the Properties that Govern Selective Ingestion and Egestion of Microplastics by the Blue Mussel (Mytilus edulis) and Eastern Oyster (Crassostrea virginica).

Suspension feeding bivalve molluscs interact with different types of microplastics (MP) suspended in the water column. Most bivalves are selective suspension feeders and, thus, do not consume all particles to which they are exposed. Selection depends upon the physicochemical properties and size of the particle. Recent work has provided evidence that blue mussels, Mytilus edulis, and eastern oysters, Crassostrea virginica, ingest and egest microspheres (polystyrene) and microfibers (nylon) differently, but whether other factors, such as polymer type and shape, mediate selection have not been explored. To investigate these factors, mussels and oysters were offered similar sized nylon (Ny) and polyester (PES) microfibers or polyethylene (PE) and polystyrene (PS) microspheres, or different sized PES microfibers during a 2 h exposure. Feces and pseudofeces were collected separately and analyzed for MPs, and the data were used to develop a linear regression model for selection. Results demonstrated clear species-specific differences in the efficiency of particle selection. Both mussels and oysters, however, exhibited size-based rejection of PES microfibers, ingesting a higher proportion of shorter fibers than longer fibers. Polymer type did not impact selection of fibers or spheres. The relative size of particles (area and perimeter) was found to be the most important factor in predicting whether a MP will be rejected or ingested.

Selective Ingestion and Egestion of Plastic Particles by the Blue Mussel (Mytilus edulis) and Eastern Oyster (Crassostrea virginica): Implications for Using Bivalves as Bioindicators of Microplastic Pollution.

Microplastics (MP; 1 µm to 1 mm) of various shapes and compositions are ingested by numerous marine animals. Recently, proposals have been made to adopt bivalve molluscs as bioindicators of MP pollution. To serve as indicators of MP pollution, however, the proposed organisms should ingest, without bias, the majority of plastic particles to which they are exposed. To test this premise, eastern oysters, Crassostrea virginica, and blue mussels, Mytilus edulis, were offered variously sized polystyrene microspheres (diameters 19-1000 µm) and nylon microfibers (lengths 75-1075 × diameter 30 µm), and the proportion of each rejected in pseudofeces and egested in feces was determined. For both species, the proportion of microspheres rejected increased from ca. 10-30% for the smallest spheres to 98% for the largest spheres. A higher proportion of the largest microsphere was rejected compared with the longest microfiber, but similar proportions of microfibers were ingested regardless of length. Differential egestion of MP also occurred. As a result of particle selection, the number and types of MP found in the bivalve gut will depend upon the physical characteristics of the particles. Thus, bivalves will be poor bioindicators of MP pollution in the environment, and it is advised that other marine species be explored.